ASAH3L (ACER2) (NM_001010887) Human Tagged ORF Clone

CAT#: RG217746

ACER2 (tGFP-tagged) - Human alkaline ceramidase 2 (ACER2)

Product Images

Circular map for RG217746

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASAH3L
• Synonyms: ALKCDase2; ASAH3L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217746 representing NM_001010887
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCCCGCACTGGTGGGACCAGCTGCAGGCTGGTAGCTCGGAGGTGGACTGGTGCGAGGACAACT
  ACACCATCGTGCCTGCTATCGCCGAGTTCTACAACACGATCAGCAATGTCTTATTTTTCATTTTACCGCC
  CATCTGCATGTGCTTGTTTCGTCAGTATGCAACATGCTTCAACAGTGGCATCTACTTAATCTGGACTCTT
  TTGGTTGTAGTGGGAATTGGATCCGTCTACTTCCATGCAACCCTTAGTTTCTTGGGTCAGATGCTTGATG
  AACTTGCAGTCCTTTGGGTTCTGATGTGTGCTTTGGCCATGTGGTTCCCCAGAAGGTATCTACCAAAGAT
  CTTTCGGAATGACCGGGGTAGGTTCAAGGTGGTGGTCAGTGTCCTGTCTGCGGTTACGACGTGCCTGGCA
  TTTGTCAAGCCTGCCATCAACAACATCTCTCTGATGACCCTGGGAGTTCCTTGCACTGCACTGCTCATCG
  CAGAGCTAAAGAGGTGTGACAACATGCGTGTGTTTAAGCTGGGCCTCTTCTCGGGCCTCTGGTGGACCCT
  GGCCCTGTTCTGCTGGATCAGTGACCGAGCTTTCTGCGAGCTGCTGTCATCCTTCAACTTCCCCTACCTG
  CACTGCATGTGGCACATCCTCATCTGCCTTGCTGCCTACCTGGGCTGTGTATGCTTTGCCTACTTTGATG
  CTGCCTCAGAGATTCCTGAGCAAGGCCCTGTCATCAAGTTCTGGCCCAATGAGAAATGGGCCTTCATTGG
  TGTCCCCTATGTGTCCCTCCTGTGTGCCAACAAGAAATCATCAGTCAAGATCACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217746 representing NM_001010887
  Red=Cloning site Green=Tags(s)
  
  MGAPHWWDQLQAGSSEVDWCEDNYTIVPAIAEFYNTISNVLFFILPPICMCLFRQYATCFNSGIYLIWTL
  LVVVGIGSVYFHATLSFLGQMLDELAVLWVLMCALAMWFPRRYLPKIFRNDRGRFKVVVSVLSAVTTCLA
  FVKPAINNISLMTLGVPCTALLIAELKRCDNMRVFKLGLFSGLWWTLALFCWISDRAFCELLSSFNFPYL
  HCMWHILICLAAYLGCVCFAYFDAASEIPEQGPVIKFWPNEKWAFIGVPYVSLLCANKKSSVKIT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010887
• ORF Size: 825 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010887.3
• RefSeq Size: 2852 bp
• RefSeq ORF: 828 bp
• Locus ID: 340485
• UniProt ID: Q5QJU3
• Cytogenetics: 9p22.1
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]).[supplied by OMIM, Jul 2010]