DOK1 (NM_001381) Human Tagged ORF Clone

CAT#: RG217617

DOK1 (tGFP-tagged) - Human docking protein 1, 62kDa (downstream of tyrosine kinase 1) (DOK1), transcript variant 1

Product Images

Circular map for RG217617

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DOK1
• Synonyms: P62DOK; pp62
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217617 representing NM_001381
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGAGCAGTGATGGAAGGGCCGCTTTTTTTGCAGAGTCAGCGCTTTGGGACCAAGAGGTGGAGGA
  AGACCTGGGCCGTGCTCTACCCGGCCAGTCCCCACGGCGTAGCGCGGCTCGAGTTCTTTGACCATAAGGG
  GTCGAGCTCTGGGGGTGGCCGAGGGAGCTCGCGCCGCCTGGACTGCAAAGTGATCCGTCTGGCTGAGTGT
  GTGAGTGTGGCCCCCGTCACCGTGGAGACCCCCCCTGAGCCCGGCGCCACTGCCTTCCGCCTGGACACTG
  CTCAGCGCTCGCACCTGCTGGCGGCCGACGCGCCGTCCAGTGCAGCCTGGGTGCAGACGCTGTGCCGAAA
  CGCCTTTCCGAAAGGCAGCTGGACTCTGGCGCCTACCGATAACCCACCTAAGCTTTCTGCCCTGGAGATG
  CTGGAGAACTCCTTGTACAGCCCTACCTGGGAAGGATCCCAATTCTGGGTAACGGTGCAGAGGACTGAGG
  CCGCCGAGCGCTGTGGCCTGCATGGCTCCTACGTGCTGAGGGTGGAGGCTGAAAGGCTGACTCTCCTGAC
  CGTGGGGGCCCAGAGTCAGATACTGGAGCCACTCCTGTCCTGGCCCTACACTCTGTTGCGTCGCTATGGC
  CGGGACAAGGTCATGTTCTCTTTCGAGGCCGGCCGCCGCTGCCCCTCAGGCCCTGGAACCTTCACCTTCC
  AGACGGCACAGGGAAATGACATCTTCCAGGCAGTTGAGACTGCCATCCACCGGCAGAAGGCCCAGGGAAA
  GGCCGGACAGGGGCACGATGTTCTCAGAGCTGACTCCCATGAAGGGGAGGTGGCAGAGGGGAAGTTGCCT
  TCCCCACCTGGCCCCCAAGAGCTCCTCGACAGTCCCCCAGCCCTGTATGCTGAGCCCTTAGACTCCCTGC
  GCATTGCTCCATGCCCTTCCCAGGACTCCCTATACTCAGACCCCTTGGACAGCACGTCTGCTCAGGCAGG
  AGAGGGAGTACAACGGAAGAAACCTCTCTATTGGGACTTGTATGAGCATGCGCAGCAGCAGTTGCTGAAG
  GCCAAGCTGACAGACCCCAAAGAGGATCCCATCTATGATGAACCTGAGGGCCTGGCCCCAGTCCCTCCCC
  AGGGCCTTTATGATCTGCCTCGGGAGCCCAAGGATGCATGGTGGTGCCAAGCTCGGGTGAAGGAGGAGGG
  CTATGAGCTCCCCTACAACCCTGCCACTGATGACTACGCTGTGCCACCCCCTCGGAGCACAAAGCCCCTC
  CTTGCTCCCAAGCCCCAGGGCCCAGCCTTCCCTGAACCTGGTACTGCAACTGGCAGTGGCATCAAAAGCC
  ACAACTCAGCCCTGTACAGCCAGGTCCAGAAGAGCGGGGCCTCAGGGAGCTGGGACTGTGGGCTCTCTAG
  AGTAGGGACTGACAAGACTGGGGTCAAGTCAGAGGGCTCTACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217617 representing NM_001381
  Red=Cloning site Green=Tags(s)
  
  MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAEC
  VSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEM
  LENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYG
  RDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLP
  SPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLK
  AKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPL
  LAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001381
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001381.4
• RefSeq Size: 1908 bp
• RefSeq ORF: 1446 bp
• Locus ID: 1796
• UniProt ID: Q99704
• Cytogenetics: 2p13.1
• Domains: PH, IRS
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]