FAHD1 (NM_031208) Human Tagged ORF Clone

CAT#: RG217597

FAHD1 (tGFP-tagged) - Human fumarylacetoacetate hydrolase domain containing 1 (FAHD1), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for RG217597

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FAHD1
• Synonyms: C16orf36; YISKL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217597 representing NM_031208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAATCATGGCAGCATCCAGGCCATTGTCCCGCTTCTGGGAGTGGGGAAAGAACATCGTCTGCGTGG
  GGAGGAACTACGCGGACCACGTCAGGGAGATGCGCAGCGCGGTGTTGAGCGAGCCCGTGCTGTTCCTGAA
  GCCGTCCACGGCCTACGCGCCCGAGGGCTCGCCCATCCTCATGCCCGCGTACACTCGCAACCTGCACCAC
  GAGCTGGAGCTGGGCGTGGTGATGGGCAAGCGCTGCCGCGCAGTCCCCGAGGCTGCGGCCATGGACTACG
  TGGGCGGCTATGCCCTGTGCCTGGATATGACCGCCCGGGACGTGCAGGACGAGTGCAAGAAGAAGGGGCT
  GCCCTGGACTCTGGCGAAGAGCTTCACGGCGTCCTGCCCGGTCAGCGCGTTCGTGCCCAAGGAGAAGATC
  CCTGACCCTCACAAGCTGAAGCTCTGGCTCAAGGTCAACGGCGAACTCAGACAGGAGGGTGAGACATCCT
  CCATGATTTTTTCCATCCCCTACATCATCAGCTATGTTTCTAAGATCATAACCTTGGAAGAAGGAGATAT
  TATCTTGACTGGGACGCCAAAGGGAGTTGGACCGGTTAAAGAAAACGATGAGATCGAGGCTGGCATACAC
  GGGCTGGTCAGTATGACATTTAAAGTGGAAAAGCCAGAATAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217597 representing NM_031208
  Red=Cloning site Green=Tags(s)
  
  MGIMAASRPLSRFWEWGKNIVCVGRNYADHVREMRSAVLSEPVLFLKPSTAYAPEGSPILMPAYTRNLHH
  ELELGVVMGKRCRAVPEAAAMDYVGGYALCLDMTARDVQDECKKKGLPWTLAKSFTASCPVSAFVPKEKI
  PDPHKLKLWLKVNGELRQEGETSSMIFSIPYIISYVSKIITLEEGDIILTGTPKGVGPVKENDEIEAGIH
  GLVSMTFKVEKPEY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031208
• ORF Size: 672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031208.3, NP_112485.1
• RefSeq Size: 1706 bp
• RefSeq ORF: 675 bp
• Locus ID: 81889
• UniProt ID: Q6P587
• Cytogenetics: 16p13.3
• Gene Summary: Probable mitochondrial acylpyruvase which is able to hydrolyze acetylpyruvate and fumarylpyruvate in vitro (PubMed:15551868, PubMed:21878618). Also has oxaloacetate decarboxylase activity (PubMed:25575590).[UniProtKB/Swiss-Prot Function]