NDST3 (NM_004784) Human Tagged ORF Clone

CAT#: RG217551

NDST3 (tGFP-tagged) - Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3)

Product Images

Circular map for RG217551

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NDST3
• Synonyms: HSST3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217551 representing NM_004784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTTTATCATGAAGCTTCACAGACACTTTCAAAGAACAGTCATTCTGCTTGCCACTTTTTGTATGG
  TGAGCATTATCATTTCTGCTTACTACCTGTACAGTGGCTACAAACAGGAAAATGAACTCTCTGAGACGGC
  TTCAGAAGTTGACTGTGGCGACCTCCAACACCTACCATATCAACTAATGGAAGTGAAAGCAATGAAGCTT
  TTTGATGCCTCAAGGACAGACCCCACAGTCCTAGTATTTGTAGAGAGCCAGTACTCATCTCTTGGTCAAG
  ACATCATTATGATTCTAGAATCAAGTAGATTCCAGTATCACATTGAAATTGCCCCTGGAAAGGGAGATCT
  CCCAGTGCTTATAGACAAAATGAAAGGCAAATACATTCTCATTATTTATGAGAATATTTTAAAGTATATA
  AATATGGATTCCTGGAATCGAAGCCTTCTAGATAAATACTGTGTAGAATATGGTGTGGGTGTCATTGGAT
  TCCACAAAACTAGTGAGAAGAGTGTACAGAGCTTTCAGTTAAAAGGTTTCCCTTTTTCCATATATGGAAA
  TCTTGCAGTAAAAGATTGTTGTATTAATCCTCATTCTCCATTGATTCGTGTGACCAAATCTTCCAAGCTT
  GAAAAAGGTTCTTTACCTGGAACTGACTGGACAGTTTTTCAGATTAATCATTCAGCCTATCAACCAGTAA
  TATTTGCCAAAGTAAAGACCCCAGAAAACCTTTCTCCTTCCATCTCTAAAGGTGCTTTTTATGCCACTAT
  TATACATGACCTGGGGCTTCATGATGGAATTCAAAGGGTTCTTTTTGGCAACAACTTGAACTTTTGGCTG
  CACAAGCTCATCTTCATAGATGCCATCTCCTTCTTATCAGGGAAGAGGCTGACATTGTCCTTGGACAGGT
  ACATTCTTGTGGATATTGATGATATATTTGTGGGAAAAGAGGGAACAAGAATGAACACCAATGATGTAAA
  GGCCCTGCTTGATACTCAGAATCTTTTGCGTGCACAAATCACAAATTTTACATTCAACCTGGGATTTTCA
  GGGAAATTTTACCATACAGGAACTGAAGAGGAAGATGAAGGAGATGACTGTCTGTTGGGGTCTGTGGATG
  AGTTCTGGTGTTTTCCTCACATGTGGAGCCATATGCAGCCCCACCTCTTCCACAATGAGTCATCTTTGGT
  GGAGCAGATGATTCTCAACAAAAAATTTGCCTTAGAGCACGGCATTCCAACGGACATGGGCTACGCTGTG
  GCCCCTCACCATTCGGGCGTCTACCCTGTACATGTTCAGCTTTACGAGGCCTGGAAGAAGGTCTGGAATA
  TTAAAATCACCAGCACTGAAGAATATCCACATCTGAAGCCAGCTAGATACCGGAGGGGTTTTATCCACAA
  AAACATCATGGTTCTCCCAAGACAAACCTGTGGGCTTTTCACTCACACCATTTTCTACAAAGAATATCCA
  GGGGGTCCTAAAGAGCTGGATAAGAGTATCCAAGGAGGAGAACTTTTCTTCACTGTCGTCCTCAACCCTA
  TCAGCATTTTCATGACCCATTTGTCCAACTATGGGAATGACCGACTGGGATTATATACATTTGTTAATCT
  GGCCAACTTTGTGAAGAGCTGGACCAACCTGCGACTTCAGACTCTGCCTCCAGTACAACTGGCCCACAAG
  TATTTTGAGCTGTTTCCTGATCAGAAAGACCCTCTCTGGCAGAATCCTTGCGATGACAAACGCCACAGAG
  ACATTTGGTCTAAAGAAAAAACTTGTGATCGCTTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGG
  TACCACTGCTTTGTATTTGTTCCTGGTTATGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAAACC
  TTTGAGGAGGTACAGTTCTTTAATAGAAATAACTACCACAGGGGGATTGATTGGTATATGGATTTCTTCC
  CAGTCCCATCTAATGTCACTACCGACTTTTTGTTTGAGAAGAGTGCCAATTACTTCCACTCAGAGGAAGC
  CCCTAAAAGAGCTGCTTCTCTGGTTCCCAAAGCCAAGATTATCACCATTCTCATTGACCCTTCAGACCGA
  GCATACTCCTGGTACCAGCATCAGCGATCACATGAAGAYCCTGCAGCTCTGAAGTTTAGCTTCTACGAAG
  TGATCTCAGCAGGGCCCCGTGCACCCTCGGAGCTCAGAGCCTTGCAGAAGAGATGTTTGGTCCCGGGGTG
  GTATGCCAGCCACATCGAGAGATGGCTTGTTTATTTCCCCCCATTTCAGTTGCTAATTATTGATGGGCAA
  CAACTAAGAACTGATCCTGCTACAGTGATGGATGAAGTACAGAAGTTTCTAGGAGTCTTGCCTCATTATA
  ATTACTCAGAAGCTTTAACGTTTGATTCTCATAAAGGTTTCTGGTGTCAGTTACTGGAAGAAGGTAAAAC
  AAAATGCCTTGGAAAGAGCAAAGGAAGAAAATACCCTCCAATGGATTCTGATAGCAGGACATTTCTGTCA
  AGCTACTATCGAGATCACAACGTGGAACTCTCAAAGCTGCTGCACAAACTGGGTCAGCCTCTGCCATCCT
  GGCTGAGACAGGAGCTGCAGAAAGTAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217551 representing NM_004784
  Red=Cloning site Green=Tags(s)
  
  MSFIMKLHRHFQRTVILLATFCMVSIIISAYYLYSGYKQENELSETASEVDCGDLQHLPYQLMEVKAMKL
  FDASRTDPTVLVFVESQYSSLGQDIIMILESSRFQYHIEIAPGKGDLPVLIDKMKGKYILIIYENILKYI
  NMDSWNRSLLDKYCVEYGVGVIGFHKTSEKSVQSFQLKGFPFSIYGNLAVKDCCINPHSPLIRVTKSSKL
  EKGSLPGTDWTVFQINHSAYQPVIFAKVKTPENLSPSISKGAFYATIIHDLGLHDGIQRVLFGNNLNFWL
  HKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNTNDVKALLDTQNLLRAQITNFTFNLGFS
  GKFYHTGTEEEDEGDDCLLGSVDEFWCFPHMWSHMQPHLFHNESSLVEQMILNKKFALEHGIPTDMGYAV
  APHHSGVYPVHVQLYEAWKKVWNIKITSTEEYPHLKPARYRRGFIHKNIMVLPRQTCGLFTHTIFYKEYP
  GGPKELDKSIQGGELFFTVVLNPISIFMTHLSNYGNDRLGLYTFVNLANFVKSWTNLRLQTLPPVQLAHK
  YFELFPDQKDPLWQNPCDDKRHRDIWSKEKTCDRLPKFLVIGPQKTGTTALYLFLVMHPSILSNSPSPKT
  FEEVQFFNRNNYHRGIDWYMDFFPVPSNVTTDFLFEKSANYFHSEEAPKRAASLVPKAKIITILIDPSDR
  AYSWYQHQRSHEXPAALKFSFYEVISAGPRAPSELRALQKRCLVPGWYASHIERWLVYFPPFQLLIIDGQ
  QLRTDPATVMDEVQKFLGVLPHYNYSEALTFDSHKGFWCQLLEEGKTKCLGKSKGRKYPPMDSDSRTFLS
  SYYRDHNVELSKLLHKLGQPLPSWLRQELQKVR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004784
• ORF Size: 2619 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004784.1, NP_004775.1
• RefSeq Size: 3188 bp
• RefSeq ORF: 2622 bp
• Locus ID: 9348
• UniProt ID: O95803
• Cytogenetics: 4q26
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. This monomeric bifunctional enzyme catalyzes the N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin, which are the initial chemical modifications required for the biosynthesis of the functional oligosaccharide sequences that define the specific ligand binding activities of heparan sulfate and heparin. [provided by RefSeq, Nov 2008]