AOC2 (NM_001158) Human Tagged ORF Clone

CAT#: RG217318

AOC2 (tGFP-tagged) - Human amine oxidase, copper containing 2 (retina-specific) (AOC2), transcript variant 1

Product Images

Circular map for RG217318

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AOC2
• Synonyms: DAO2; RAO; SSAO
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217318 representing NM_001158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTCAAGATAGTCCTGGCGTTCCTGGCACTGTCCCTCATTACCATCTTTGCCCTGGCCTATGTTT
  TGCTGACCAGCCCAGGTGGTTCCAGCCAGCCTCCCCACTGCCCCTCTGTATCCCATAGGGCCCAGCCCTG
  GCCACACCCTGGCCAGAGCCAGCTGTTTGCAGACCTGAGCCGAGAGGAGTTGACAGCTGTGATGCGCTTT
  CTGACCCAGCGGCTGGGGCCAGGGCTGGTGGACGCAGCCCAGGCTCAGCCCTCGGACAACTGCATCTTCT
  CAGTGGAGCTGCAGCTGCCCCCCAAGGCTGCAGCCCTGGCCCACCTGGACAGGGGGAGCCCCCCACCTGC
  CCGGGAGGCACTGGCCATCGTCCTCTTTGGTGGACAACCCCAACCCAATGTGAGTGAGCTGGTGGTGGGG
  CCGCTGCCTCACCCCTCGTACATGCGGGATGTGACTGTGGAGCGTCACGGCGGGCCCCTGCCCTATCACC
  GTCGCCCGGTGCTGAGAGCTGAGTTTACACAGATGTGGAGGCATCTGAAAGAGGTGGAGCTACCCAAGGC
  ACCCATCTTCCTGTCGTCCACCTTCAACTACAATGGCTCTACCCTGGCAGCTGTGCATGCCACCCCTCGG
  GGCTTGCGCTCAGGGGACCGAGCTACCTGGATGGCCCTCTACCATAACATCTCAGGGGTTGGTCTTTTCC
  TTCACCCCGTGGGGCTGGAGCTACTACTGGACCACAGGGCCCTGGACCCTGCCCACTGGACTGTCCAGCA
  GGTCTTCTACCTTGGGCACTACTATGCAGACTTGGGCCAGTTGGAACGGGAGTTTAAGTCTGGCCGGTTG
  GAAGTGGTTAGAGTCCCTCTACCTCCACCAAATGGAGCTTCATCCCTGAGGTCTCGGAACTCTCCAGGTC
  CTCTTCCCCCTCTTCAGTTCTCGCCCCAGGGTTCCCAGTACAGTGTGCAAGGAAACCTGGTGGTATCCTC
  CCTCTGGTCATTTACCTTTGGCCATGGGGTGTTCAGCGGCCTGAGGATTTTTGATGTTCGGTTCCAGGGT
  GAGCGAATAGCCTATGAAGTCAGTGTCCAGGAGTGTGTATCTATCTATGGTGCCGATTCACCCAAGACGA
  TGCTGACTCGCTATTTGGATAGCAGCTTTGGACTCGGCCGTAACAGCCGAGGCTTGGTGCGGGGAGTGGA
  CTGCCCCTATCAAGCCACGATGGTGGACATCCATATATTAGTGGGCAAAGGGGCAGTCCAGCTGCTTCCA
  GGGGCTGTGTGTGTATTTGAGGAAGCCCAGGGACTGCCCCTTCGAAGGCACCACAATTACCTTCAAAATC
  ATTTCTATGGTGGTTTGGCCAGCTCAGCCCTTGTGGTCAGGTCTGTGTCATCTGTGGGCAACTATGACTA
  CATTTGGGACTTTGTGTTGTACCCAAATGGGGCACTTGAAGGGCGGGTCCATGCCACGGGTTATATCAAC
  ACAGCTTTCCTGAAAGGGGGAGAGGAGGGCCTCCTCTTTGGGAACCGTGTGGGGGAAAGAGTGCTGGGAA
  CGGTGCACACACATGCCTTCCACTTCAAGCTGGACCTGGATGTGGCAGGGCTGAAAAACTGGGTGGTAGC
  TGAAGACGTGGTGTTTAAACCTGTGGCTGCCCCCTGGAACCCGGAGCACTGGCTACAGCGCCCACAGCTG
  ACTCGGCAGGTCCTGGGAAAGGAGGACCTGACAGCTTTTTCCTTGGGAAGCCCCCTACCCCGCTACCTCT
  ACCTGGCTAGCAACCAGACTAATGCGTGGGGTCACCAGCGCGGATACCAGCTTGTGGTGACCCAGAGAAA
  GGAGGAGGAGTCACAGAGCAGTAGCATCTATCACCAGAATGACATCTGGACACCCACAGTTACCTTTGCT
  GACTTCATCAACAATGAAACCCTCTTAGGAGAGGATCTGGTGGCTTGGGTCACAGCCAGCTTCCTGCACA
  TTCCCCATGCCGAGGACATCCCAAACACAGTGACTCTGGGGAACAGAGTTGGCTTCTTGCTCCGACCCTA
  TAACTTCTTTGATGAGGACCCCTCCATCTTCTCCCCTGGCAGTGTCTACTTTGAGAAGGGCCAGGATGCT
  GGGCTCTGCAGCATCAATCCTGTGGCCTGCCTCCCCGACCTGGCAGCCTGTGTCCCGGACTTACCCCCTT
  TCTCTTACCACGGCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217318 representing NM_001158
  Red=Cloning site Green=Tags(s)
  
  MHLKIVLAFLALSLITIFALAYVLLTSPGGSSQPPHCPSVSHRAQPWPHPGQSQLFADLSREELTAVMRF
  LTQRLGPGLVDAAQAQPSDNCIFSVELQLPPKAAALAHLDRGSPPPAREALAIVLFGGQPQPNVSELVVG
  PLPHPSYMRDVTVERHGGPLPYHRRPVLRAEFTQMWRHLKEVELPKAPIFLSSTFNYNGSTLAAVHATPR
  GLRSGDRATWMALYHNISGVGLFLHPVGLELLLDHRALDPAHWTVQQVFYLGHYYADLGQLEREFKSGRL
  EVVRVPLPPPNGASSLRSRNSPGPLPPLQFSPQGSQYSVQGNLVVSSLWSFTFGHGVFSGLRIFDVRFQG
  ERIAYEVSVQECVSIYGADSPKTMLTRYLDSSFGLGRNSRGLVRGVDCPYQATMVDIHILVGKGAVQLLP
  GAVCVFEEAQGLPLRRHHNYLQNHFYGGLASSALVVRSVSSVGNYDYIWDFVLYPNGALEGRVHATGYIN
  TAFLKGGEEGLLFGNRVGERVLGTVHTHAFHFKLDLDVAGLKNWVVAEDVVFKPVAAPWNPEHWLQRPQL
  TRQVLGKEDLTAFSLGSPLPRYLYLASNQTNAWGHQRGYQLVVTQRKEEESQSSSIYHQNDIWTPTVTFA
  DFINNETLLGEDLVAWVTASFLHIPHAEDIPNTVTLGNRVGFLLRPYNFFDEDPSIFSPGSVYFEKGQDA
  GLCSINPVACLPDLAACVPDLPPFSYHGF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001158
• ORF Size: 2187 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001158.5
• RefSeq Size: 2600 bp
• RefSeq ORF: 2190 bp
• Locus ID: 314
• UniProt ID: O75106
• Cytogenetics: 17q21.31
• Protein Families: Transmembrane
• Protein Pathways: beta-Alanine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Phenylalanine metabolism, Tyrosine metabolism
• Gene Summary: Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes and ammonia in the presence of copper and quinone cofactor. This gene shows high sequence similarity to copper amine oxidases from various species ranging from bacteria to mammals. The protein contains several conserved motifs including the active site of amine oxidases and the histidine residues that likely bind copper. It may be a critical modulator of signal transmission in retina, possibly by degrading the biogenic amines dopamine, histamine, and putrescine. This gene may be a candidate gene for hereditary ocular diseases. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]