PIK3C2G (NM_004570) Human Tagged ORF Clone

CAT#: RG217086

PIK3C2G (tGFP-tagged) - Human phosphoinositide-3-kinase, class 2, gamma polypeptide (PIK3C2G)

Product Images

Circular map for RG217086

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIK3C2G
• Synonyms: PI3K-C2-gamma; PI3K-C2GAMMA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217086 representing NM_004570
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATATTCTTGGCAAACGGATCCAAATCCTAATGAATCACACGAAAAGCAGTATGAACACCAAGAAT
  TTCTCTTTGTAAATCAACCCCATTCTTCTAGCCAAGTCAGTCTGGGTTTTGATCAGATAGTAGATGAGAT
  CAGTGGCAAAATTCCACACTACGAGAGTGAAATTGATGAAAACACCTTTTTTGTGCCCACTGCACCAAAA
  TGGGACTCAACAGGGCATTCATTAAATGAAGCACACCAAATATCCTTGAATGAATTCACTTCTAAAAGCC
  GTGAACTCTCCTGGCATCAAGTTAGCAAAGCACCAGCAATTGGTTTTAGTCCTTCTGTGTTACCAAAACC
  TCAAAATACGAATAAAGAATGCTCCTGGGGAAGCATAGGAAAACATCATGGTGCTGATGATTCCAGATTC
  AGTATTTTAGCTCTATCATTCACAAGTTTGGATAAAATTAATCTAGAGAAAGAATTAGAAAATGAAAATC
  ATAACTACCATATAGGATTTGAAAGTAGCATTCCTCCAACAAATTCATCCTTCTCAAGTGACTTCATGCC
  GAAAGAAGAGAATAAAAGGAGTGGACATGTGAACATTGTGGAACCATCTTTGATGCTTTTGAAAGGCTCT
  CTTCAACCCGGAATGTGGGAAAGTACATGGCAGAAGAATATAGAGTCAATAGGTTGTTCCATTCAGCTAG
  TGGAAGTACCTCAAAGCAGCAATACGAGTCTGGCCTCTTTTTGCAACAAAGTAAAAAAAATCAGAGAAAG
  ATATCATGCAGCTGATGTTAATTTCAATTCTGGGAAGATCTGGAGCACTACTACAGCATTTCCGTATCAG
  CTCTTTTCTAAGACCAAGTTTAATATACATATTTTTATTGATAACTCAACACAACCTCTTCATTTTATGC
  CATGTGCTAATTATCTTGTCAAAGATCTAATTGCAGAAATTCTGCATTTTTGCACAAATGACCAGCTACT
  CCCCAAAGATCATATTCTAAGTGTATGTGGCTCTGAAGAATTTTTACAAAACGACCACTGTTTGGGGAGC
  CACAAAATGTTTCAAAAAGATAAATCTGTTATTCAGCTCCACCTGCAGAAAAGTAGGGAAGCTCCAGGAA
  AGCTATCTCGAAAGCATGAAGAGGACCACAGTCAGTTTTATCTGAATCAACTTCTAGAATTTATGCATAT
  TTGGAAAGTATCCAGACAATGTCTCTTAACACTCATCAGAAAATATGACTTCCACCTGAAATACCTATTG
  AAAACCCAGGAAAACGTGTATAATATTATTGAAGAAGTTAAAAAAATATGCAGTGTTCTAGGGTGTGTGG
  AAACCAAACAAATTACAGATGCAGTAAATGAACTAAGTCTAATTCTTCAGAGAAAAGGAGAGAATTTTTA
  TCAAAGTTCAGAGACTTCAGCAAAAGGCTTGATAGAGAAGGTAACAACTGAACTATCCACATCCATCTAC
  CAGCTAATCAATGTCTACTGTAACAGCTTTTATGCAGATTTTCAGCCTGTAAATGTACCTAGATGCACTT
  CCTATCTAAATCCCGGGCTTCCTTCCCACCTCAGCTTCACAGTGTATGCAGCACACAACATTCCAGAAAC
  CTGGGTGCACAGCTACAAAGCGTTTTCTTTTACCTGTTGGCTTACATATGCTGGAAAGAAGCTGTGCCAA
  GTGAGAAACTACAGAAATATTCCAGACAAGAAATTATTTTTTTTCTTGGTCAACTGGAATGAAACGATCA
  ATTTTCCCCTTGAAATAAAGTCACTTCCAAGGGAATCCATGCTCACTGTAAAACTGTTTGGGATTGCCTG
  TGCAACCAACAATGCAAATTTACTGGCGTGGACTTGTCTTCCACTGTTTCCAAAAGAAAAATCCATTCTC
  GGGTCTATGCTGTTCAGCATGACATTACAGAGTGAGCCTCCCGTAGAAATGATAACTCCAGGAGTGTGGG
  ATGTAAGTCAGCCATCCCCGGTGACCCTGCAGATTGATTTTCCAGCTACTGGGTGGGAGTATATGAAACC
  TGATTCTGAAGAGAATAGAAGTAATCTTGAAGAGCCACTAAAGGAGTGTATAAAACATATTGCCAGACTT
  TCACAGAAACAGACTCCCCTACTACTCTCTGAAGAAAAGAAAAGATATTTATGGTTTTATCGCTTCTACT
  GCAATAATGAAAACTGCTCCCTTCCTTTAGTCCTGGGTAGTGCCCCTGGATGGGATGAAAGGACTGTTTC
  AGAAATGCATACCATTTTGAGAAGATGGACATTTTCTCAACCTTTAGAGGCTCTTGGGCTTTTGACTTCC
  AGTTTTCCAGATCAAGAAATTCGTAAAGTGGCAGTTCAACAATTAGACAACCTCTTGAATGATGAACTAC
  TGGAATATCTCCCACAGCTAGTTCAGGCTGTCAAGTTTGAATGGAACCTTGAGAGTCCTTTAGTGCAACT
  TCTACTCCACCGCTCCTTGCAGAGCATCCAGGTTGCCCATCGTCTTTACTGGCTGCTAAAAAATGCAGAA
  AATGAAGCTTATTTTAAAAGCTGGTATCAGAAGCTACTAGCTGCTCTCCAATTCTGTGCAGGTAAAGCCT
  TGAATGATGAGTTTTCCAAGGAGCAGAAACTTATCAAAATTCTGGGAGATATTGGGGAAAGAGTCAAGTC
  TGCCAGTGACCATCAAAGACAGGAGGTACTGAAGAAAGAAATTGGCAGACTAGAAGAGTTCTTTCAAGAT
  GTAAATACTTGTCATCTTCCTCTGAACCCTGCCCTATGTATAAAAGGGATTGATCACGATGCATGTTCAT
  ATTTTACATCTAATGCTTTGCCATTGAAGATTACTTTCATCAATGCTAATCCGATGGGCAAAAACATCAG
  CATTATTTTTAAGGCTGGAGATGATCTTCGTCAGGATATGCTTGTTCTGCAGCTTATTCAAGTGATGGAC
  AATATTTGGCTGCAGGAAGGCTTGGATATGCAAATGATCATTTATAGATGTCTATCCACAGGAAAAGACC
  AAGGATTGGTGCAGATGGTACCTGATGCTGTGACCCTAGCAAAGATTCATCGCCATTCTGGACTGATAGG
  ACCATTGAAAGAAAATACAATTAAAAAGTGGTTCAGTCAGCACAACCACTTAAAGGCAGATTATGAAAAG
  GCCTTGAGGAACTTTTTCTACTCCTGTGCTGGCTGGTGTGTGGTAACATTCATCCTGGGAGTATGTGACC
  GTCACAATGATAATATCATGCTGACAAAGTCGGGCCACATGTTTCATATTGACTTTGGAAAATTCTTAGG
  TCATGCACAAACATTTGGAGGGATAAAAAGGGACCGAGCTCCTTTCATTTTTACTTCAGAGATGGAATAC
  TTTATTACAGAGGGTGGGAAAAACCCACAGCATTTTCAAGATTTTGTGGAACTTTGCTGTCGTGCTTATA
  ATATTATCAGAAAGCACAGCCAACTGCTCTTGAACCTGCTGGAAATGATGCTGTATGCAGGACTGCCTGA
  GCTAAGTGGAATTCAAGACCTGAAATATGTGTATAATAATCTTCGTCCACAAGACACAGACCTGGAAGCA
  ACAAGTCATTTTACCAAGAAAATAAAGGAAAGTCTGGAGTGTTTCCCTGTTAAATTGAATAACTTGATCC
  ACACACTTGCACAAATGTCAGCCATAAGCCCTGCCAAATCTACTTCACAGACTTTTCCTCAGGAATCCTG
  TTTGCTGAGTACAACTAGGTCGATTGAAAGAGCAACAATTTTAGGGTTCAGCAAGAAATCCAGTAATCTG
  TATCTGATCCAGGTGACACACAGCAACAACGAAACAAGCCTGACAGAAAAATCATTTGAGCAGTTTTCAA
  AACTTCACAGCCAACTTCAGAAGCAGTTTGCATCACTGACTCTCCCAGAGTTTCCTCATTGGTGGCACCT
  ACCTTTTACAAATTCAGATCACAGAAGATTCAGAGATCTAAATCATTACATGGAACAGATATTAAATGTA
  TCACATGAAGTTACAAACAGTGATTGTGTACTTAGCTTTTTCCTCTCTGAGGCTGTGCAACAAACAGTTG
  AAGAATCATCACCTGTGTACCTAGGTGAGAAGTTTCCAGACAAGAAGCCTAAGGTGCAGTTAGTCATATC
  CTACGAGGATGTGAAGCTGACCATACTAGTGAAACACATGAAAAACATTCATCTCCCAGATGGCTCTGCG
  CCCAGTGCACATGTTGAATTTTATCTTTTACCATATCCCAGTGAAGTTCGTAGGAGGAAAACAAAATCTG
  TTCCAAAATGTACGGACCCCACTTACAATGAAATTGTAGTATATGATGAAGTCACAGAGCTCCAAGGACA
  TGTCTTAATGCTTATTGTGAAGAGTAAAACTGTATTTGTGGGAGCAATTAACATCCGACTCTGTAGTGTC
  CCACTCGATAAAGAAAAATGGTATCCATTAGGAAACAGTATAATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217086 representing NM_004570
  Red=Cloning site Green=Tags(s)
  
  MAYSWQTDPNPNESHEKQYEHQEFLFVNQPHSSSQVSLGFDQIVDEISGKIPHYESEIDENTFFVPTAPK
  WDSTGHSLNEAHQISLNEFTSKSRELSWHQVSKAPAIGFSPSVLPKPQNTNKECSWGSIGKHHGADDSRF
  SILALSFTSLDKINLEKELENENHNYHIGFESSIPPTNSSFSSDFMPKEENKRSGHVNIVEPSLMLLKGS
  LQPGMWESTWQKNIESIGCSIQLVEVPQSSNTSLASFCNKVKKIRERYHAADVNFNSGKIWSTTTAFPYQ
  LFSKTKFNIHIFIDNSTQPLHFMPCANYLVKDLIAEILHFCTNDQLLPKDHILSVCGSEEFLQNDHCLGS
  HKMFQKDKSVIQLHLQKSREAPGKLSRKHEEDHSQFYLNQLLEFMHIWKVSRQCLLTLIRKYDFHLKYLL
  KTQENVYNIIEEVKKICSVLGCVETKQITDAVNELSLILQRKGENFYQSSETSAKGLIEKVTTELSTSIY
  QLINVYCNSFYADFQPVNVPRCTSYLNPGLPSHLSFTVYAAHNIPETWVHSYKAFSFTCWLTYAGKKLCQ
  VRNYRNIPDKKLFFFLVNWNETINFPLEIKSLPRESMLTVKLFGIACATNNANLLAWTCLPLFPKEKSIL
  GSMLFSMTLQSEPPVEMITPGVWDVSQPSPVTLQIDFPATGWEYMKPDSEENRSNLEEPLKECIKHIARL
  SQKQTPLLLSEEKKRYLWFYRFYCNNENCSLPLVLGSAPGWDERTVSEMHTILRRWTFSQPLEALGLLTS
  SFPDQEIRKVAVQQLDNLLNDELLEYLPQLVQAVKFEWNLESPLVQLLLHRSLQSIQVAHRLYWLLKNAE
  NEAYFKSWYQKLLAALQFCAGKALNDEFSKEQKLIKILGDIGERVKSASDHQRQEVLKKEIGRLEEFFQD
  VNTCHLPLNPALCIKGIDHDACSYFTSNALPLKITFINANPMGKNISIIFKAGDDLRQDMLVLQLIQVMD
  NIWLQEGLDMQMIIYRCLSTGKDQGLVQMVPDAVTLAKIHRHSGLIGPLKENTIKKWFSQHNHLKADYEK
  ALRNFFYSCAGWCVVTFILGVCDRHNDNIMLTKSGHMFHIDFGKFLGHAQTFGGIKRDRAPFIFTSEMEY
  FITEGGKNPQHFQDFVELCCRAYNIIRKHSQLLLNLLEMMLYAGLPELSGIQDLKYVYNNLRPQDTDLEA
  TSHFTKKIKESLECFPVKLNNLIHTLAQMSAISPAKSTSQTFPQESCLLSTTRSIERATILGFSKKSSNL
  YLIQVTHSNNETSLTEKSFEQFSKLHSQLQKQFASLTLPEFPHWWHLPFTNSDHRRFRDLNHYMEQILNV
  SHEVTNSDCVLSFFLSEAVQQTVEESSPVYLGEKFPDKKPKVQLVISYEDVKLTILVKHMKNIHLPDGSA
  PSAHVEFYLLPYPSEVRRRKTKSVPKCTDPTYNEIVVYDEVTELQGHVLMLIVKSKTVFVGAINIRLCSV
  PLDKEKWYPLGNSII
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004570
• ORF Size: 4344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004570.2, NP_004561.1
• RefSeq Size: 4855 bp
• RefSeq ORF: 4338 bp
• Locus ID: 5288
• UniProt ID: O75747
• Cytogenetics: 12p12.3
• Domains: C2, PI3_PI4_kinase, PI3Ka, PX, PI3K_C2
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. This gene may play a role in several diseases, including type II diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]