HCN4 (NM_005477) Human Tagged ORF Clone

CAT#: RG216770

HCN4 (tGFP-tagged) - Human hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4)

Product Images

Circular map for RG216770

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HCN4
• Synonyms: SSS2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216770 representing NM_005477
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGCTGCCGCCGTCCATGCGCAAGCGGCTCTACAGCCTCCCGCAGCAGGTGGGGGCCAAGGCGT
  GGATCATGGACGAGGAAGAGGACGCCGAGGAGGAGGGGGCCGGGGGCCGCCAAGACCCCAGCCGCAGGAG
  CATCCGGCTGCGGCCACTGCCCTCGCCCTCCCCCTCGGCGGCCGCGGGTGGCACGGAGTCCCGGAGCTCG
  GCCCTCGGGGCAGCGGACAGCGAAGGGCCGGCCCGCGGCGCGGGCAAGTCCAGCACGAACGGCGACTGCA
  GGCGCTTCCGCGGGAGCCTGGCCTCGCTGGGCAGCCGGGGCGGCGGCAGCGGCGGCACGGGGAGCGGCAG
  CAGTCACGGACACCTGCATGACTCCGCGGAGGAGCGGCGGCTCATCGCCGAGGGCGACGCGTCCCCCGGC
  GAGGACAGGACGCCCCCAGGCCTGGCGGCCGAGCCCGAGCGCCCCGGCGCCTCGGCGCAGCCCGCAGCCT
  CGCCGCCGCCGCCCCAGCAGCCACCGCAGCCGGCCTCCGCCTCCTGCGAGCAGCCCTCGGTGGACACCGC
  TATCAAAGTGGAGGGAGGCGCGGCTGCCGGCGACCAGATCCTCCCGGAGGCCGAGGTGCGCCTGGGCCAG
  GCCGGCTTCATGCAGCGCCAGTTCGGGGCCATGCTCCAACCCGGGGTCAACAAATTCTCCCTAAGGATGT
  TCGGCAGCCAGAAAGCCGTGGAGCGCGAACAGGAGAGGGTCAAGTCGGCCGGATTTTGGATTATCCACCC
  CTACAGTGACTTCAGATTTTACTGGGACCTGACCATGCTGCTGCTGATGGTGGGAAACCTGATTATCATT
  CCTGTGGGCATCACCTTCTTCAAGGATGAGAACACCACACCCTGGATTGTCTTCAATGTGGTGTCAGACA
  CATTCTTCCTCATCGACTTGGTCCTCAACTTCCGCACAGGGATCGTGGTGGAGGACAACACAGAGATCAT
  CCTGGACCCGCAGCGGATTAAAATGAAGTACCTGAAAAGCTGGTTCATGGTAGATTTCATTTCCTCCATC
  CCCGTGGACTACATCTTCCTCATTGTGGAGACACGCATCGACTCGGAGGTCTACAAGACTGCCCGGGCCC
  TGCGCATTGTCCGCTTCACGAAGATCCTCAGCCTCTTACGCCTGTTACGCCTCTCCCGCCTCATTCGATA
  TATTCACCAGTGGGAAGAGATCTTCCACATGACCTACGACCTGGCCAGCGCCGTGGTGCGCATCGTGAAC
  CTCATCGGCATGATGCTCCTGCTCTGCCACTGGGACGGCTGCCTGCAGTTCCTGGTACCCATGCTACAGG
  ACTTCCCTGACGACTGCTGGGTGTCCATCAACAACATGGTGAACAACTCCTGGGGGAAGCAGTACTCCTA
  CGCGCTCTTCAAGGCCATGAGCCACATGCTGTGCATCGGCTACGGGCGGCAGGCGCCCGTGGGCATGTCC
  GACGTCTGGCTCACCATGCTCAGCATGATCGTGGGTGCCACCTGCTACGCCATGTTCATTGGCCACGCCA
  CTGCCCTCATCCAGTCCCTGGACTCCTCCCGGCGCCAGTACCAGGAAAAGTACAAGCAGGTGGAGCAGTA
  CATGTCCTTTCACAAGCTCCCGCCCGACACCCGGCAGCGCATCCACGACTACTACGAGCACCGCTACCAG
  GGCAAGATGTTCGACGAGGAGAGCATCCTGGGCGAGCTAAGCGAGCCCCTGCGGGAGGAGATCATCAACT
  TTAACTGTCGGAAGCTGGTGGCCTCCATGCCACTGTTTGCCAATGCGGACCCCAACTTCGTGACGTCCAT
  GCTGACCAAGCTGCGTTTCGAGGTCTTCCAGCCTGGGGACTACATCATCCGGGAAGGCACCATTGGCAAG
  AAGATGTACTTCATCCAGCATGGCGTGGTCAGCGTGCTCACCAAGGGCAACAAGGAGACCAAGCTGGCCG
  ACGGCTCCTACTTTGGAGAGATCTGCCTGCTGACCCGGGGCCGGCGCACAGCCAGCGTGAGGGCCGACAC
  CTACTGCCGCCTCTACTCGCTGAGCGTGGACAACTTCAATGAGGTGCTGGAGGAGTACCCCATGATGCGA
  AGGGCCTTCGAGACCGTGGCGCTGGACCGCCTGGACCGCATTGGCAAGAAGAACTCCATCCTCCTCCACA
  AAGTCCAGCACGACCTCAACTCCGGCGTCTTCAACTACCAGGAGAATGAGATCATCCAGCAGATTGTGCA
  GCATGACCGGGAGATGGCCCACTGCGCGCACCGCGTCCAGGCTGCTGCCTCTGCCACCCCAACCCCCACG
  CCCGTCATCTGGACCCCGCTGATCCAGGCACCACTGCAGGCTGCCGCTGCCACCACTTCTGTGGCCATAG
  CCCTCACCCACCACCCTCGCCTGCCTGCTGCCATCTTCCGCCCTCCCCCAGGATCTGGGCTGGGCAACCT
  CGGTGCCGGGCAGACGCCAAGGCACCTGAAACGGCTGCAGTCCCTGATCCCTTCTGCGCTGGGCTCCGCC
  TCGCCCGCCAGCAGCCCGTCCCAGGTGGACACACCGTCTTCATCCTCCTTCCACATCCAACAGCTGGCTG
  GATTCTCTGCCCCCGCTGGACTGAGCCCACTCCTGCCCTCATCCAGCTCCTCCCCACCCCCCGGGGCCTG
  TGGCTCCCCCTCGGCTCCCACACCATCAGCTGGCGTAGCCGCCACCACCATAGCCGGGTTTGGCCACTTC
  CACAAGGCGCTGGGTGGCTCCCTGTCCTCCTCCGACTCTCCCCTGCTCACCCCGCTGCAGCCAGGCGCCC
  GCTCCCCGCAGGCTGCCCAGCCATCTCCCGCGCCACCCGGGGCCCGGGGAGGCCTGGGACTCCCGGAGCA
  CTTCCTGCCACCCCCACCCTCATCCAGATCCCCGTCATCTAGCCCCGGGCAGCTGGGCCAGCCTCCCGGG
  GAGTTGTCCCTAGGTCTGGCCACTGGCCCACTGAGCACGCCAGAGACACCCCCACGGCAGCCTGAGCCGC
  CGTCCCTTGTGGCAGGGGCCTCTGGGGGGGCTTCCCCTGTAGGCTTTACTCCCCGAGGAGGTCTCAGCCC
  CCCTGGCCACAGCCCAGGCCCCCCAAGAACCTTCCCGAGTGCCCCGCCCCGGGCCTCTGGCTCCCACGGA
  TCCTTGCTCCTGCCACCTGCATCCAGCCCCCCACCACCCCAGGTCCCCCAGCGCCGGGGCACACCCCCGC
  TCACCCCCGGCCGCCTCACCCAGGACCTCAAGCTCATCTCCGCGTCTCAGCCAGCCCTGCCTCAGGACGG
  GGCGCAGACTCTCCGCAGAGCCTCCCCGCACTCCTCAGGGGAGTCCATGGCTGCCTTCCCGCTCTTCCCC
  AGGGCTGGGGGTGGCAGCGGGGGCAGTGGGAGCAGCGGGGGCCTCGGTCCCCCTGGGAGGCCCTATGGTG
  CCATCCCCGGCCAGCACGTCACTCTGCCTCGGAAGACATCCTCAGGTTCTTTGCCACCCCCTCTGTCTTT
  GTTTGGGGCAAGAGCCACCTCTTCTGGGGGGCCCCCTCTGACTGCTGGACCCCAGAGGGAACCTGGGGCC
  AGGCCTGAGCCAGTGCGCTCCAAACTGCCATCCAATCTA
  
  CTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216770 representing NM_005477
  Red=Cloning site Green=Tags(s)
  
  MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTESRSS
  ALGAADSEGPARGAGKSSTNGDCRRFRGSLASLGSRGGGSGGTGSGSSHGHLHDSAEERRLIAEGDASPG
  EDRTPPGLAAEPERPGASAQPAASPPPPQQPPQPASASCEQPSVDTAIKVEGGAAAGDQILPEAEVRLGQ
  AGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIII
  PVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFMVDFISSI
  PVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVN
  LIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMS
  DVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQ
  GKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGK
  KMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMR
  RAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPT
  PVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGSGLGNLGAGQTPRHLKRLQSLIPSALGSA
  SPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGACGSPSAPTPSAGVAATTIAGFGHF
  HKALGGSLSSSDSPLLTPLQPGARSPQAAQPSPAPPGARGGLGLPEHFLPPPPSSRSPSSSPGQLGQPPG
  ELSLGLATGPLSTPETPPRQPEPPSLVAGASGGASPVGFTPRGGLSPPGHSPGPPRTFPSAPPRASGSHG
  SLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESMAAFPLFP
  RAGGGSGGSGSSGGLGPPGRPYGAIPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAGPQREPGA
  RPEPVRSKLPSNL
  
  LE - GFP Tag - V
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005477
• ORF Size: 3609 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005477.3
• RefSeq Size: 5065 bp
• RefSeq ORF: 3612 bp
• Locus ID: 10021
• UniProt ID: Q9Y3Q4
• Cytogenetics: 15q24.1
• Domains: cNMP, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Cyclic nucleotide gated, Transmembrane
• Gene Summary: This gene encodes a member of the hyperpolarization-activated cyclic nucleotide-gated potassium channels. The encoded protein shows slow kinetics of activation and inactivation, and is necessary for the cardiac pacemaking process. This channel may also mediate responses to sour stimuli. Mutations in this gene have been linked to sick sinus syndrome 2, also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. Two pseudogenes have been identified on chromosome 15. [provided by RefSeq, Oct 2008]