TAF1 (NM_138923) Human Tagged ORF Clone

CAT#: RG216684

TAF1 (tGFP-tagged) - Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 2

Product Images

Circular map for RG216684

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TAF1
• Synonyms: BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216684 representing NM_138923
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCCGGCTGCGATTTGCTGCTGCGGACAGCAGCTACCATCACTGCTGCCGCCATCATGTCAGACA
  CGGACAGCGACGAAGATTCCGCTGGAGGCGGCCCATTTTCTTTAGCGGGTTTCCTTTTCGGCAACATCAA
  TGGAGCCGGGCAGCTGGAGGGGGAAAGCGTCTTGGATGATGAATGTAAGAAGCACTTGGCAGGCTTGGGG
  GCTTTGGGGCTGGGCAGCCTGATCACTGAACTCACGGCAAATGAAGAATTGACCGGGACTGACGGTGCCT
  TGGTAAATGATGAAGGGTGGGTTAGGAGTACAGAAGATGCTGTGGACTATTCAGACATCAATGAGGTGGC
  AGAAGATGAAAGCCGAAGATACCAGCAGACGATGGGGAGCTTGCAGCCCCTTTGCCACTCAGATTATGAT
  GAAGATGACTATGATGCTGATTGTGAAGACATTGATTGCAAGTTGATGCCTCCTCCACCTCCACCCCCGG
  GACCAATGAAGAAGGATAAGGACCAGGATTCTATTACTGGTGAGAAAGTGGACTTCAGTAGTTCCTCTGA
  CTCAGAATCTGAGATGGGACCTCAGGAAGCAACACAGGCAGAATCTGAAGATGGAAAGCTGACCCTTCCA
  TTGGCTGGGATTATGCAGCATGATGCCACCAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTC
  GACCTGGAAAGGTGTTACGTTTTCTACGTCTTTTTGGACCAGGGAAGAATGTCCCATCTGTTTGGCGGAG
  TGCTCGGAGAAAGAGGAAGAAGAAGCACCGTGAGCTGATACAGGAAGAGCAGATCCAGGAGGTGGAGTGC
  TCAGTAGAATCAGAAGTCAGCCAGAAGTCTTTGTGGAACTACGACTACGCTCCACCACCACCTCCAGAGC
  AGTGTCTCTCTGATGATGAAATCACGATGATGGCTCCTGTGGAGTCCAAATTTTCCCAATCAACTGGAGA
  TATAGATAAAGTGACAGATACCAAACCAAGAGTGGCTGAGTGGCGTTATGGGCCTGCCCGACTGTGGTAT
  GATATGCTGGGTGTCCCTGAAGATGGCAGTGGGTTTGACTATGGCTTCAAACTGAGAAAGACAGAACATG
  AACCTGTGATAAAATCTAGAATGATAGAGGAATTTAGGAAACTTGAGGAAAACAATGGCACTGATCTTCT
  GGCTGATGAAAACTTCCTGATGGTGACACAGCTGCATTGGGAGGATGATATCATCTGGGATGGGGAGGAT
  GTCAAACACAAAGGGACAAAACCTCAGCGTGCAAGCCTGGCAGGCTGGCTTCCTTCTAGCATGACTAGGA
  ATGCGATGGCTTACAATGTTCAGCAAGGTTTTGCAGCCACTCTTGATGATGACAAACCTTGGTACTCCAT
  TTTTCCCATTGACAATGAGGATCTGGTATATGGACGCTGGGAGGACAATATCATTTGGGATGCTCAGGCC
  ATGCCCCGGCTGTTGGAACCTCCTGTTTTGACACTTGATCCCAATGATGAGAACCTCATTTTGGAAATTC
  CTGATGAGAAGGAAGAGGCCACCTCTAACTCCCCCTCCAAGGAGAGTAAGAAGGAATCATCTCTGAAGAA
  GAGTCGAATTCTCTTAGGGAAAACAGGAGTCATCAAGGAGGAACCACAGCAGAACATGTCTCAGCCAGAA
  GTGAAAGATCCATGGAATCTCTCCAATGATGAGTATTATTATCCCAAGCAACAGGGTCTTCGAGGCACCT
  TTGGAGGGAATATTATCCAGCATTCAATTCCTGCTGTGGAATTACGGCAGCCCTTCTTTCCCACCCACAT
  GGGGCCCATCAAACTCCGGCAGTTCCATCGCCCACCTCTGAAAAAGTACTCATTTGGTGCACTTTCTCAG
  CCAGGTCCCCACTCAGTCCAACCTTTGCTAAAGCACATCAAAAAAAAGGCCAAGATGAGAGAACAAGAGA
  GGCAAGCTTCAGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGACCTCACAGGCAAAGATGGTGA
  TCTTATTCTTGCAGAATATAGTGAGGAAAATGGACCCTTAATGATGCAGGTTGGCATGGCAACCAAGATA
  AAGAACTATTATAAACGGAAACCTGGAAAAGATCCTGGAGCACCAGATTGTAAATATGGGGAAACTGTTT
  ACTGCCATACATCTCCTTTCCTGGGTTCTCTCCATCCTGGCCAATTGCTGCAAGCATTTGAGAACAACCT
  TTTTCGTGCTCCAATTTATCTTCATAAGATGCCAGAAACTGATTTCTTGATCATTCGGACAAGACAGGGT
  TACTATATTCGGGAATTAGTGGATATTTTTGTGGTTGGCCAGCAGTGTCCCTTGTTTGAAGTTCCTGGGC
  CTAACTCCAAAAGGGCCAATACGCATATTCGAGACTTTCTACAGGTTTTTATTTACCGCCTTTTCTGGAA
  AAGTAAAGATCGGCCACGGAGGATACGAATGGAAGATATAAAAAAAGCCTTTCCTTCCCATTCAGAAAGC
  AGCATCCGGAAGAGGCTAAAGCTCTGCGCTGACTTCAAACGCACAGGGATGGACTCAAACTGGTGGGTGC
  TTAAGTCTGATTTTCGTTTACCAACGGAAGAAGAGATCAGAGCTATGGTGTCACCAGAGCAGTGCTGTGC
  TTATTATAGCATGATAGCTGCAGAGCAACGACTGAAGGATGCTGGCTATGGTGAGAAATCCTTTTTTGCT
  CCAGAAGAAGAAAATGAGGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGCACTGCCCCTTGGAACA
  CCACAAGGGCCTTCATTGCTGCCATGAAGGGCAAGTGTCTGCTAGAGGTGACTGGGGTGGCAGATCCCAC
  GGGGTGTGGTGAAGGATTCTCCTATGTGAAGATTCCAAACAAACCAACACAGCAGAAGGATGATAAAGAA
  CCGCAGCCAGTGAAGAAGACAGTGACAGGAACAGATGCAGACCTTCGTCGCCTTTCCCTGAAAAATGCCA
  AGCAACTTCTACGTAAATTTGGTGTGCCTGAGGAAGAGATTAAAAAGTTGTCCCGCTGGGAAGTGATTGA
  TGTGGTGCGCACAATGTCAACAGAACAGGCTCGTTCTGGAGAGGGGCCCATGAGTAAATTTGCCCGTGGA
  TCAAGGTTTTCTGTGGCTGAGCATCAAGAGCGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGA
  ACAAGGTTCTGTCATCAACTGAAGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGATAGTGACTT
  TGAAGAAATGGGAAAGAACATTGAGAACATGTTGCAGAACAAGAAAACCAGCTCTCAGCTTTCACGTGAA
  CGGGAGGAACAGGAGCGGAAGGAACTACAGCGAATGCTACTGGCAGCAGGCTCAGCAGCATCCGGAAACA
  ATCACAGAGATGATGACACAGCTTCCGTGACTAGCCTTAACTCTTCTGCCACTGGACGCTGTCTCAAGAT
  TTATCGCACGTTTCGAGATGAAGAGGGGAAAGAGTATGTTCGCTGTGAGACAGTCCGAAAACCAGCTGTC
  ATTGATGCCTATGTGCGCATACGGACTACAAAAGATGAGGAATTCATTCGAAAATTTGCCCTTTTTGATG
  AACAACATCGGGAAGAGATGCGAAAAGAACGGCGGAGGATTCAAGAGCAACTGAGGCGGCTTAAGAGGAA
  CCAGGAAAAGGAGAAGCTTAAGGGTCCTCCTGAGAAGAAGCCCAAGAAAATGAAGGAGCGTCCTGACCTA
  AAACTGAAATGTGGGGCATGTGGTGCCATTGGACACATGAGGACTAACAAATTCTGCCCCCTCTATTATC
  AAACAAATGCGCCACCTTCCAACCCTGTTGCCATGACAGAAGAACAGGAGGAGGAGTTGGAAAAGACAGT
  CATTCATAATGATAATGAAGAACTTATCAAGGTTGAAGGGACCAAAATTGTCTTGGGGAAACAGCTAATT
  GAGAGTGCGGATGAGGTTCGCAGAAAATCTCTGGTTCTCAAGTTTCCTAAACAGCAGCTTCCTCCAAAGA
  AGAAACGGCGAGTTGGAACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAGTCCATCCACCGGCG
  CCGCACAGACCCTATGGTGACGCTGTCGTCCATCTTGGAGTCTATCATCAATGACATGAGAGATCTTCCA
  AATACATACCCTTTCCACACTCCAGTCAATGCAAAGGTTGTAAAGGACTACTACAAAATCATCACTCGGC
  CAATGGACCTACAAACACTCCGCGAAAACGTGCGTAAACGCCTCTACCCATCTCGGGAAGAGTTCAGAGA
  GCATCTGGAGCTAATTGTGAAAAATAGTGCAACCTACAATGGGCCAAAACACTCATTGACTCAGATCTCT
  CAATCCATGCTGGATCTCTGTGATGAAAAACTCAAAGAGAAAGAAGACAAATTAGCTCGCTTAGAGAAAG
  CTATCAACCCCTTGCTGGATGATGATGACCAAGTGGCGTTTTCTTTCATTCTGGACAACATTGTCACCCA
  GAAAATGATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTAT
  TACAAAGTGATTGTCAATCCAATGGATTTAGAGACCATACGTAAGAACATCTCCAAGCACAAGTATCAGA
  GTCGGGAGAGCTTTCTGGATGATGTAAACCTTATTCTGGCCAACAGTGTTAAGTATAATGGACCTGAGAG
  TCAGTATACTAAGACTGCCCAGGAGATTGTGAACGTCTGTTACCAGACATTGACTGAGTATGATGAACAT
  TTGACTCAACTTGAGAAGGATATTTGTACTGCTAAAGAAGCAGCTTTGGAGGAAGCAGAATTAGAAAGCC
  TGGACCCAATGACCCCAGGGCCCTACACGCCTCAGCCTCCTGATTTGTATGATACCAACACATCCCTCAG
  TATGTCTCGAGATGCCTCTGTATTTCAAGATGAGAGCAATATGTCTGTCTTGGATATTCCCAGTGCCACT
  CCAGAAAAGCAGGTAACACAGGAAGGTGAAGATGGAGATGGTGATCTTGCAGATGAAGAGGAAGGAACTG
  TACAACAGCCTCAAGCCAGTGTCCTGTATGAGGATTTGCTTATGTCTGAAGGAGAAGATGATGAGGAAGA
  TGCTGGGAGTGATGAAGAAGGAGACAATCCTTTCTCTGCTATCCAGCTGAGTGAAAGTGGAAGTGACTCT
  GATGTGGGATCTGGTGGAATAAGACCCAAACAACCCCGCATGCTTCAGGAGAACACAAGGATGGACATGG
  AAAATGAAGAAAGCATGATGTCCTATGAGGGAGACGGTGGGGAGGCTTCCCATGGTTTGGAGGATAGCAA
  CATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCGAACACCCAAGACACAAGCTTCAGCAGCATC
  GGTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAGGAGGAAGAGCAGCGCTCTGGGCCGAGCGTAC
  TAAGCCAGGTCCACCTGTCAGAGGACGAGGAGGACAGTGAGGATTTCCACTCCATTGCTGGGGACAGTGA
  CTTGGACTCTGATGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216684 representing NM_138923
  Red=Cloning site Green=Tags(s)
  
  MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLG
  ALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHSDYD
  EDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGEKVDFSSSSDSESEMGPQEATQAESEDGKLTLP
  LAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVEC
  SVESEVSQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWY
  DMLGVPEDGSGFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNGTDLLADENFLMVTQLHWEDDIIWDGED
  VKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEDLVYGRWEDNIIWDAQA
  MPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPE
  VKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQ
  PGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKI
  KNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQG
  YYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSES
  SIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFA
  PEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKE
  PQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARG
  SRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSRE
  REEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAV
  IDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDL
  KLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLI
  ESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLP
  NTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQIS
  QSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDY
  YKVIVNPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEH
  LTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPSAT
  PEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDS
  DVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSI
  GGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138923
• ORF Size: 5616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138923.3
• RefSeq Size: 7646 bp
• RefSeq ORF: 5559 bp
• Locus ID: 6872
• UniProt ID: P21675
• Cytogenetics: Xq13.1
• Protein Families: Protein Kinase
• Protein Pathways: Basal transcription factors
• Gene Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]