HIP1R (NM_003959) Human Tagged ORF Clone

CAT#: RG216638

HIP1R (tGFP-tagged) - Human huntingtin interacting protein 1 related (HIP1R)

Product Images

Circular map for RG216638

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HIP1R
• Synonyms: HIP3; HIP12; ILWEQ
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216638 representing NM_003959
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCATCAAGAACGTGCCGGCGCGGGTGCTGAGCCGCAGGCCGGGCCACAGCCTGGAGGCCGAGC
  GCGAGCAGTTCGACAAGACCCAGGCCATCAGCATCAGCAAAGCCATCAACACCCAGGAGGCCCCCGTGAA
  GGAGAAGCACGCCCGGCGCATCATTCTGGGCACACACCACGAGAAGGGGGCTTTCACCTTCTGGTCCTAT
  GCCATTGGGCTGCCGCTGCCCAGCAGCTCCATTCTCAGCTGGAAGTTCTGCCACGTCCTCCACAAGGTCC
  TTCGAGACGGGCACCCCAATGTGCTGCATGACTGCCAGCGGTACCGCAGCAACATCCGGGAGATTGGAGA
  CCTGTGGGGACATTTGCATGACCGCTACGGACAGCTGGTGAATGTCTACACCAAGCTGCTGCTGACCAAG
  ATCTCCTTCCACCTCAAGCATCCCCAGTTTCCCGCGGGCCTGGAGGTGACAGATGAGGTACTGGAGAAGG
  CAGCTGGGACCGATGTCAACAACATCTTCCAGCTCACTGTGGAGATGTTTGATTACATGGATTGTGAGCT
  GAAGCTTTCTGAATCAGTTTTCCGACAGCTCAACACGGCCATCGCCGTATCCCAGATGTCCTCAGGCCAG
  TGCCGCCTGGCCCCCCTCATCCAGGTCATCCAGGACTGCAGCCACCTCTACCACTACACGGTCAAGCTCC
  TGTTCAAGCTACACTCTTGTCTCCCTGCGGACACCCTGCAAGGCCACAGGGACCGGTTCCACGAGCAGTT
  TCACAGCCTCAGGAACTTCTTCCGCAGAGCCTCCGACATGCTGTACTTCAAGCGGCTCATCCAGATCCCC
  CGGCTGCCCGAGGGACCCCCTAACTTCCTGCGGGCCTCAGCCCTGGCTGAGCACATCAAGCCGGTGGTGG
  TGATCCCCGAGGAGGCCCCGGAAGATGAGGAGCCGGAGAATCTCATTGAGATCAGCACAGGGCCCCCCGC
  GGGGGAGCCAGTGGTGGTGGCTGACCTCTTCGATCAGACGTTTGGACCCCCCAATGGGTCTGTGAAGGAC
  GACAGGGACCTCCAGATTGAGAGCTTGAAGAGAGAGGTGGAAATGCTCCGCTCTGAACTGGAGAAGATCA
  AGCTGGAGGCCCAGCGGTACATCGCGCAGCTGAAGAGCCAGGTGAATGCACTGGAGGGTGAGCTGGAGGA
  GCAGCGGAAGCAGAAGCAGAAGGCCCTGGTGGATAATGAGCAGCTCCGCCACGAGCTGGCCCAGCTGAGG
  GCTGCCCAGCTGGAGGGCGAGCGGAGCCAGGGCCTGCGTGAGGAGGCTGAGAGGAAGGCCAGTGCCACGG
  AGGCGCGCTACAACAAGCTGAAGGAAAAGCACAGTGAGCTCGTCCATGTGCACGCGGAGCTGCTCAGAAA
  GAACGCGGACACAGCCAAGCAGCTGACGGTGACGCAGCAAAGCCAGGAGGAGGTGGCGCGGGTGAAGGAG
  CAGCTGGCCTTCCAGGTGGAGCAGGTGAAGCGGGAGTCGGAGTTGAAGCTAGAGGAGAAGAGCGACCAGC
  TGGAGAAGCTCAAGAGGGAGCTGGAGGCCAAGGCCGGAGAGCTGGCCCGCGCGCAGGAGGCCCTGAGCCA
  CACAGAGCAGAGCAAGTCGGAGCTGAGCTCACGGCTGGACACGCTGAGTGCGGAGAAGGATGCTCTGAGT
  GGAGCTGTGCGGCAGCGGGAGGCAGACCTGCTGGCGGCGCAGAGCCTGGTGCGCGAGACAGAGGCGGCGC
  TGAGCCGGGAGCAGCAGCGCAGCTCCCAGGAGCAGGGCGAGTTGCAGGGCCGGCTGGCAGAGAGGGAGTC
  TCAGGAGCAGGGGCTGCGGCAGAGGCTGCTGGACGAGCAGTTCGCAGTGTTGCGGGGCGCTGCTGCCGAG
  GCCGCGGGCATCCTGCAGGATGCCGTGAGCAAGCTGGACGACCCCCTGCACCTGCGCTGTACCAGCTCCC
  CAGACTACCTGGTGAGCAGGGCCCAGGAGGCCTTGGATGCCGTGAGCACCCTGGAGGAGGGCCACGCCCA
  GTACCTGACCTCCTTGGCAGACGCCTCCGCCCTGGTGGCAGCTCTGACCCGCTTCTCCCACCTGGCTGCG
  GATACCATCATCAATGGCGGTGCCACCTCGCACCTGGCTCCCACCGACCCTGCCGACCGCCTCATAGACA
  CCTGCAGGGAGTGCGGGGCCCGGGCTCTGGAGCTCATGGGGCAGCTGCAGGACCAGCAGGCTCTGCGGCA
  CATGCAGGCCAGCCTGGTGCGGACACCCCTGCAGGGCATCCTTCAGCTGGGCCAGGAACTGAAACCCAAG
  AGCCTAGATGTGCGGCAGGAGGAGCTGGGGGCCGTGGTCGACAAGGAGATGGCGGCCACATCCGCAGCCA
  TTGAAGATGCTGTGCGGAGGATTGAGGACATGATGAACCAGGCACGCCACGCCAGCTCGGGGGTGAAGCT
  GGAGGTGAACGAGAGGATCCTCAACTCCTGCACAGACCTGATGAAGGCTATCCGGCTCCTGGTGACGACA
  TCCACTAGCCTGCAGAAGGAGATCGTGGAGAGCGGCAGGGGGGCAGCCACGCAGCAGGAATTTTACGCCA
  AGAACTCGCGCTGGACCGAAGGCCTCATCTCGGCCTCCAAGGCTGTGGGCTGGGGAGCCACACAGCTGGT
  GGAGGCAGCTGACAAGGTGGTGCTTCACACGGGCAAGTATGAGGAGCTCATCGTCTGCTCCCACGAGATC
  GCAGCCAGCACGGCCCAGCTGGTGGCGGCCTCCAAGGTGAAGGCCAACAAGCACAGCCCCCACCTGAGCC
  GCCTGCAGGAATGTTCTCGCACAGTCAATGAGAGGGCTGCCAATGTGGTGGCCTCCACCAAGTCAGGCCA
  GGAGCAGATTGAGGACAGAGACACCATGGATTTCTCCGGCCTGTCCCTCATCAAGCTGAAGAAGCAGGAG
  ATGGAGACGCAGGTGCGTGTCCTGGAGCTGGAGAAGACGCTGGAGGCTGAACGCATGCGGCTGGGGGAGT
  TGCGGAAGCAACACTACGTGCTGGCTGGGGCATCAGGCAGCCCTGGAGAGGAGGTGGCCATCCGGCCCAG
  CACTGCCCCCCGAAGTGTAACCACCAAGAAACCACCCCTGGCCCAGAAGCCCAGCGTGGCCCCCAGACAG
  GACCACCAGCTTGACAAAAAGGATGGCATCTACCCAGCTCAACTCGTGAACTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216638 representing NM_003959
  Red=Cloning site Green=Tags(s)
  
  MNSIKNVPARVLSRRPGHSLEAEREQFDKTQAISISKAINTQEAPVKEKHARRIILGTHHEKGAFTFWSY
  AIGLPLPSSSILSWKFCHVLHKVLRDGHPNVLHDCQRYRSNIREIGDLWGHLHDRYGQLVNVYTKLLLTK
  ISFHLKHPQFPAGLEVTDEVLEKAAGTDVNNIFQLTVEMFDYMDCELKLSESVFRQLNTAIAVSQMSSGQ
  CRLAPLIQVIQDCSHLYHYTVKLLFKLHSCLPADTLQGHRDRFHEQFHSLRNFFRRASDMLYFKRLIQIP
  RLPEGPPNFLRASALAEHIKPVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPNGSVKD
  DRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQKALVDNEQLRHELAQLR
  AAQLEGERSQGLREEAERKASATEARYNKLKEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKE
  QLAFQVEQVKRESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLDTLSAEKDALS
  GAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQGRLAERESQEQGLRQRLLDEQFAVLRGAAAE
  AAGILQDAVSKLDDPLHLRCTSSPDYLVSRAQEALDAVSTLEEGHAQYLTSLADASALVAALTRFSHLAA
  DTIINGGATSHLAPTDPADRLIDTCRECGARALELMGQLQDQQALRHMQASLVRTPLQGILQLGQELKPK
  SLDVRQEELGAVVDKEMAATSAAIEDAVRRIEDMMNQARHASSGVKLEVNERILNSCTDLMKAIRLLVTT
  STSLQKEIVESGRGAATQQEFYAKNSRWTEGLISASKAVGWGATQLVEAADKVVLHTGKYEELIVCSHEI
  AASTAQLVAASKVKANKHSPHLSRLQECSRTVNERAANVVASTKSGQEQIEDRDTMDFSGLSLIKLKKQE
  METQVRVLELEKTLEAERMRLGELRKQHYVLAGASGSPGEEVAIRPSTAPRSVTTKKPPLAQKPSVAPRQ
  DHQLDKKDGIYPAQLVNY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003959
• ORF Size: 3204 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003959.1, NP_003950.1
• RefSeq Size: 4473 bp
• RefSeq ORF: 3207 bp
• Locus ID: 9026
• UniProt ID: O75146
• Cytogenetics: 12q24.31
• Gene Summary: Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.[UniProtKB/Swiss-Prot Function]