PTPN13 (NM_080684) Human Tagged ORF Clone

CAT#: RG216356

PTPN13 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase) (PTPN13), transcript variant 3

Product Images

Circular map for RG216356

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPN13
• Synonyms: FAP-1; hPTP1E; PNP1; PTP-BAS; PTP-BL; PTP1E; PTPL1; PTPLE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216356 representing NM_080684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGTGTCACTAGCTGAGGCCCTGGAGGTTCGGGGTGGACCACTTCAGGAGGAAGAAATATGGGCTG
  TATTAAATCAAAGTGCTGAAAGTCTCCAAGAATTATTCAGAAAAGTAAGCCTAGCTGATCCTGCTGCCCT
  TGGCTTCATCATTTCTCCATGGTCTCTGCTGTTGCTGCCATCTGGTAGTGTGTCATTTACAGATGAAAAT
  ATTTCCAATCAGGATCTTCGAGCATTCACTGCACCAGAGGTTCTTCAAAATCAGTCACTAACTTCTCTCT
  CAGATGTTGAAAAGATCCACATTTATTCTCTTGGAATGACACTGTATTGGGGGGCTGATTATGAAGTGCC
  TCAGAGCCAACCTATTAAGCTTGGAGATCATCTCAACAGCATACTGCTTGGAATGTGTGAGGATGTTATT
  TACGCTCGAGTTTCTGTTCGGACTGTGCTGGATGCTTGCAGTGCCCACATTAGGAATAGCAATTGTGCAC
  CCTCATTTTCCTACGTGAAACACTTGGTAAAACTGGTTCTGGGAAATCTTTCTGGGACAGATCAGCTTTC
  CTGTAACAGTGAACAAAAGCCTGATCGAAGCCAGGCTATTCGAGATCGATTGCGAGGAAAAGGATTACCA
  ACAGGAAGAAGCTCTACTTCTGATGTACTAGACATACAAAAGCCTCCACTCTCTCATCAGACCTTTCTTA
  ACAAAGGGCTTAGTAAATCTATGGGATTTCTGTCCATCAAAGATACACAAGATGAGAATTATTTCAAGGA
  CATTTTATCAGATAATTCTGGACGTGAAGATTCTGAAAATACATTCTCCCCTTACCAGTTCAAAACTAGT
  GGCCCAGAAAAAAAACCCATCCCTGGCATTGATGTGCTTTCTAAGAAGAAGATCTGGGCTTCATCCATGG
  ACTTGCTTTGTACAGCTGACAGAGACTTCTCTTCAGGAGAGACTGCCACATATCGTCGTTGTCACCCTGA
  GGCAGTAACAGTGCGGACTTCAACTACTCCTAGAAAAAAGGAGGCAAGATACTCAGATGGAAGTATAGCC
  TTGGATATCTTTGGCCCTCAGAAAATGGATCCAATATATCACACTCGAGAATTGCCCACCTCCTCAGCAA
  TATCAAGTGCTTTGGACCGAATCCGAGAGAGACAAAAGAAACTTCAGGTTCTGAGGGAAGCCATGAATGT
  AGAAGAACCAGTTCGAAGATACAAAACTTATCATGGTGATGTCTTTAGTACCTCCAGTGAAAGTCCATCT
  ATTATTTCCTCTGAATCAGATTTCAGACAAGTGAGAAGAAGTGAAGCCTCAAAGAGGTTTGAATCCAGCA
  GTGGTCTCCCAGGGGTAGATGAAACCTTAAGTCAAGGCCAGTCACAGAGACCGAGCAGACAATATGAAAC
  ACCCTTTGAAGGCAACTTAATTAATCAAGAGATCATGCTAAAACGGCAAGAGGAAGAACTGATGCAGCTA
  CAAGCCAAAATGGCCCTTAGACAGTCTCGGTTGAGCCTATATCCAGGAGACACAATCAAAGCGTCCATGC
  TTGACATCACCAGGGATCCGTTAAGAGAAATTGCCCTAGAAACAGCCATGACTCAAAGAAAACTGAGGAA
  TTTCTTTGGCCCTGAGTTTGTGAAAATGACAATTGAACCATTTATATCTTTGGATTTGCCACGGTCTATT
  CTTACTAAGAAAGGGAAGAATGAGGATAACCGAAGGAAAGTAAACATAATGCTTCTGAACGGGCAAAGAC
  TGGAACTGACCTGTGATACCAAAACTATATGTAAAGATGTGTTTGATATGGTTGTGGCACATATTGGCTT
  AGTAGAGCATCATTTGTTTGCTTTAGCTACCCTCAAAGATAATGAATATTTCTTTGTTGATCCTGACTTA
  AAATTAACCAAAGTGGCCCCAGAGGGATGGAAAGAAGAACCAAAGAAAAAGACCAAAGCCACTGTTAATT
  TTACTTTGTTTTTCAGAATTAAATTTTTTATGGATGATGTTAGTCTAATACAACATACTCTGACGTGTCA
  TCAGTATTACCTTCAGCTTCGAAAAGATATTTTGGAGGAAAGGATGCACTGTGATGATGAGACTTCCTTA
  TTGCTGGCATCCTTGGCTCTCCAGGCTGAGTATGGAGATTATCAACCAGAGGTTCATGGTGTGTCTTACT
  TTAGAATGGAGCACTATTTGCCCGCCAGAGTGATGGAGAAACTTGATTTATCCTATATCAAAGAAGAGTT
  ACCCAAATTGCATAATACCTATGTGGGAGCTTCTGAAAAAGAGACAGAGTTAGAATTTTTAAAGGTCTGC
  CAAAGACTGACAGAATATGGAGTTCATTTTCACCGAGTGCACCCTGAGAAGAAGTCACAAACAGGAATAT
  TGCTTGGAGTCTGTTCTAAAGGTGTCCTTGTGTTTGAAGTTCACAATGGAGTGCGCACATTGGTCCTTCG
  CTTTCCATGGAGGGAAACCAAGAAAATATCTTTTTCTAAAAAGAAAATCACATTGCAAAATACATCAGAT
  GGAATAAAACATGGCTTCCAGACAGACAACAGTAAGATATGCCAGTACCTGCTGCACCTCTGCTCTTACC
  AGCATAAGTTCCAGCTACAGATGAGAGCAAGACAGAGCAACCAAGATGCCCAAGATATTGATGTGCTACA
  CAAAAGATGGAGCATAGTATCTTCACCAGAAAGGGAGATCACCTTAGTGAACCTGAAAAAAGATGCAAAG
  TATGGCTTGGGATTTCAAATTATTGGTGGGGAGAAGATGGGAAGACTGGACCTAGGCATATTTATCAGTT
  CAGTTGCCCCTGGAGGACCAGCTGACTTGGATGGATGCTTGAAGCCAGGAGACCGTTTGATATCTGTGAA
  TAGTGTGAGTCTGGAGGGAGTCAGCCACCATGCTGCAATTGAAATTTTGCAAAATGCACCTGAAGATGTG
  ACACTTGTTATCTCTCAGCCAAAAGAAAAGATATCCAAAGTGCCTTCTACTCCTGTGCATCTCACCAATG
  AGATGAAAAACTACATGAAGAAATCTTCCTACATGCAAGACAGTGCTATAGATTCTTCTTCCAAGGATCA
  CCACTGGTCACGTGGTACCCTGAGGCACATCTCGGAGAACTCCTTTGGGCCATCTGGGGGCCTGCGGGAA
  GGAAGCCTGAGTTCTCAAGATTCCAGGACTGAGAGTGCCAGCTTGTCTCAAAGCCAGGTCAATGGTTTCT
  TTGCCAGCCATTTAGGTGACCAAACCTGGCAGGAATCACAGCATGGCAGCCCTTCCCCATCTGTAATATC
  CAAAGCCACCGAGAAAGAGACTTTCACTGATAGTAACCAAAGCAAAACTAAAAAGCCAGGCATTTCTGAT
  GTAACTGATTACTCAGACCGTGGAGATTCAGACATGGATGAAGCCACTTACTCCAGCAGTCAGGATCATC
  AAACACCAAAACAGGAATCTTCCTCTTCAGTGAATACATCCAACAAGATGAATTTTAAAACTTTTTCTTC
  ATCACCTCCTAAGCCTGGAGATATCTTTGAGGTTGAACTGGCTAAAAATGATAACAGCTTGGGGATAAGT
  GTCACGGGAGGTGTGAATACGAGTGTCAGACATGGTGGCATTTATGTGAAAGCTGTTATTCCCCAGGGAG
  CAGCAGAGTCTGATGGTAGAATTCACAAAGGTGATCGCGTCCTAGCTGTCAATGGAGTTAGTCTAGAAGG
  AGCCACCCATAAGCAAGCTGTGGAAACACTGAGAAATACAGGACAGGTGGTTCATCTGTTATTAGAAAAG
  GGACAATCTCCAACATCTAAAGAACATGTCCCGGTAACCCCACAGTGTACCCTTTCAGATCAGAATGCCC
  AAGGTCAAGGCCCAGAAAAAGTGAAGAAAACAACTCAGGTCAAAGACTACAGCTTTGTCACTGAAGAAAA
  TACATTTGAGGTAAAATTATTTAAAAATAGCTCAGGTCTAGGATTCAGTTTTTCTCGAGAAGATAATCTT
  ATACCGGAGCAAATTAATGCCAGCATAGTAAGGGTTAAAAAGCTCTTTCCTGGACAGCCAGCAGCAGAAA
  GTGGAAAAATTGATGTAGGAGATGTTATCTTGAAAGTGAATGGAGCCTCTTTGAAAGGACTATCTCAGCA
  GGAAGTCATATCTGCTCTCAGGGGAACTGCTCCAGAAGTATTCTTGCTTCTCTGCAGACCTCCACCTGGT
  GTGCTACCGGAAATTGATACTGCGCTTTTGACCCCACTTCAGTCTCCAGCACAAGTACTTCCAAACAGCA
  GTAAAGACTCTTCTCAGCCATCATGTGTGGAGCAAAGCACCAGCTCAGATGAAAATGAAATGTCAGACAA
  AAGCAAAAAACAGTGCAAGTCCCCATCCAGAAGAGACAGTTACAGTGACAGCAGTGGGAGTGGAGAAGAT
  GACTTAGTGACAGCTCCAGCAAACATATCAAATTCGACCTGGAGTTCAGCTTTGCATCAGACTCTAAGCA
  ACATGGTATCACAGGCACAGAGTCATCATGAAGCACCCAAGAGTCAAGAAGATACCATTTGTACCATGTT
  TTACTATCCTCAGAAAATTCCCAATAAACCAGAGTTTGAGGACAGTAATCCTTCCCCTCTACCACCGGAT
  ATGGCTCCTGGGCAGAGTTATCAACCCCAATCAGAATCTGCTTCCTCTAGTTCGATGGATAAGTATCATA
  TACATCACATTTCTGAACCAACTAGACAAGAAAACTGGACACCTTTGAAAAATGACTTGGAAAATCACCT
  TGAAGACTTTGAACTGGAAGTAGAACTCCTCATTACCCTAATTAAATCAGAAAAAGGAAGCCTGGGTTTT
  ACAGTAACCAAAGGCAATCAGAGAATTGGTTGTTATGTTCATGATGTCATACAGGATCCAGCCAAAAGTG
  ATGGAAGGCTAAAACCTGGGGACCGGCTCATAAAGGTTAATGATACAGATGTTACTAATATGACTCATAC
  AGATGCAGTTAATCTGCTCCGGGCTGCATCCAAAACAGTCAGATTAGTTATTGGACGAGTTCTAGAATTA
  CCCAGAATACCAATGTTGCCTCATTTGCTACCGGACATAACACTAACGTGCAACAAAGAGGAGTTGGGTT
  TTTCCTTATGTGGAGGTCATGACAGCCTTTATCAAGTGGTATATATTAGTGATATTAATCCAAGGTCCGT
  CGCAGCCATTGAGGGTAATCTCCAGCTATTAGATGTCATCCATTATGTGAACGGAGTCAGCACACAAGGA
  ATGACCTTGGAGGAAGTTAACAGAGCATTAGACATGTCACTTCCTTCATTGGTATTGAAAGCAACAAGAA
  ATGATCTTCCAGTGGTCCCCAGCTCAAAGAGGTCTGCTGTTTCAGCTCCAAAGTCAACCAAAGGCAATGG
  TTCCTACAGTGTGGGGTCTTGCAGCCAGCCTGCCCTCACTCCTAATGATTCATTCTCCACGGTTGCTGGG
  GAAGAAATAAATGAAATATCGTACCCCAAAGGAAAATGTTCTACTTATCAGATAAAGGGATCACCAAACT
  TGACTCTGCCCAAAGAATCTTATATACAAGAAGATGACATTTATGATGATTCCCAAGAAGCTGAAGTTAT
  CCAGTCTCTGCTGGATGTTGTGGATGAGGAAGCCCAGAATCTTTTAAACGAAAATAATGCAGCAGGATAC
  TCCTGTGGTCCAGGTACATTAAAGATGAATGGGAAGTTATCAGAAGAGAGAACAGAAGATACAGACTGCG
  ATGGTTCACCTTTACCTGAGTATTTTACTGAGGCCACCAAAATGAATGGCTGTGAAGAATATTGTGAAGA
  AAAAGTAAAAAGTGAAAGCTTAATTCAGAAGCCACAAGAAAAGAAGACTGATGATGATGAAATAACATGG
  GGAAATGATGAGTTGCCAATAGAGAGAACAAACCATGAAGATTCTGATAAAGATCATTCCTTTCTGACAA
  ACGATGAGCTCGCTGTACTCCCTGTCGTCAAAGTGCTTCCCTCTGGTAAATACACGGGTGCCAACTTAAA
  ATCAGTCATTCGAGTCCTGCGGGGTTTGCTAGATCAAGGAATTCCTTCTAAGGAGCTGGAGAATCTTCAA
  GAATTAAAACCTTTGGATCAGTGTCTAATTGGGCAAACTAAGGAAAACAGAAGGAAGAACAGATATAAAA
  ATATACTTCCCTATGATGCTACAAGAGTGCCTCTTGGAGATGAAGGTGGCTATATCAATGCCAGCTTCAT
  TAAGATACCAGTTGGGAAAGAAGAGTTCGTTTACATTGCCTGCCAAGGACCACTGCCTACAACTGTTGGA
  GACTTCTGGCAGATGATTTGGGAGCAAAAATCCACAGTGATAGCCATGATGACTCAAGAAGTAGAAGGAG
  AAAAAATCAAATGCCAGCGCTATTGGCCCAACATCCTAGGCAAAACAACAATGGTCAGCAACAGACTTCG
  ACTGGCTCTTGTGAGAATGCAGCAGCTGAAGGGCTTTGTGGTGAGGGCAATGACCCTTGAAGATATTCAG
  ACCAGAGAGGTGCGCCATATTTCTCATCTGAATTTCACTGCCTGGCCAGACCATGATACACCTTCTCAAC
  CAGATGATCTGCTTACTTTTATCTCCTACATGAGACACATCCACAGATCAGGCCCAATCATTACGCACTG
  CAGTGCTGGCATTGGACGTTCAGGGACCCTGATTTGCATAGATGTGGTTCTGGGATTAATCAGTCAGGAT
  CTTGATTTTGACATCTCTGATTTGGTGCGCTGCATGAGACTACAAAGACACGGAATGGTTCAGACAGAGG
  ATCAATATATTTTCTGCTATCAAGTCATCCTTTATGTCCTGACACGTCTTCAAGCAGAAGAAGAGCAAAA
  ACAGCAGCCTCAGCTTCTGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216356 representing NM_080684
  Red=Cloning site Green=Tags(s)
  
  MHVSLAEALEVRGGPLQEEEIWAVLNQSAESLQELFRKVSLADPAALGFIISPWSLLLLPSGSVSFTDEN
  ISNQDLRAFTAPEVLQNQSLTSLSDVEKIHIYSLGMTLYWGADYEVPQSQPIKLGDHLNSILLGMCEDVI
  YARVSVRTVLDACSAHIRNSNCAPSFSYVKHLVKLVLGNLSGTDQLSCNSEQKPDRSQAIRDRLRGKGLP
  TGRSSTSDVLDIQKPPLSHQTFLNKGLSKSMGFLSIKDTQDENYFKDILSDNSGREDSENTFSPYQFKTS
  GPEKKPIPGIDVLSKKKIWASSMDLLCTADRDFSSGETATYRRCHPEAVTVRTSTTPRKKEARYSDGSIA
  LDIFGPQKMDPIYHTRELPTSSAISSALDRIRERQKKLQVLREAMNVEEPVRRYKTYHGDVFSTSSESPS
  IISSESDFRQVRRSEASKRFESSSGLPGVDETLSQGQSQRPSRQYETPFEGNLINQEIMLKRQEEELMQL
  QAKMALRQSRLSLYPGDTIKASMLDITRDPLREIALETAMTQRKLRNFFGPEFVKMTIEPFISLDLPRSI
  LTKKGKNEDNRRKVNIMLLNGQRLELTCDTKTICKDVFDMVVAHIGLVEHHLFALATLKDNEYFFVDPDL
  KLTKVAPEGWKEEPKKKTKATVNFTLFFRIKFFMDDVSLIQHTLTCHQYYLQLRKDILEERMHCDDETSL
  LLASLALQAEYGDYQPEVHGVSYFRMEHYLPARVMEKLDLSYIKEELPKLHNTYVGASEKETELEFLKVC
  QRLTEYGVHFHRVHPEKKSQTGILLGVCSKGVLVFEVHNGVRTLVLRFPWRETKKISFSKKKITLQNTSD
  GIKHGFQTDNSKICQYLLHLCSYQHKFQLQMRARQSNQDAQDIDVLHKRWSIVSSPEREITLVNLKKDAK
  YGLGFQIIGGEKMGRLDLGIFISSVAPGGPADLDGCLKPGDRLISVNSVSLEGVSHHAAIEILQNAPEDV
  TLVISQPKEKISKVPSTPVHLTNEMKNYMKKSSYMQDSAIDSSSKDHHWSRGTLRHISENSFGPSGGLRE
  GSLSSQDSRTESASLSQSQVNGFFASHLGDQTWQESQHGSPSPSVISKATEKETFTDSNQSKTKKPGISD
  VTDYSDRGDSDMDEATYSSSQDHQTPKQESSSSVNTSNKMNFKTFSSSPPKPGDIFEVELAKNDNSLGIS
  VTGGVNTSVRHGGIYVKAVIPQGAAESDGRIHKGDRVLAVNGVSLEGATHKQAVETLRNTGQVVHLLLEK
  GQSPTSKEHVPVTPQCTLSDQNAQGQGPEKVKKTTQVKDYSFVTEENTFEVKLFKNSSGLGFSFSREDNL
  IPEQINASIVRVKKLFPGQPAAESGKIDVGDVILKVNGASLKGLSQQEVISALRGTAPEVFLLLCRPPPG
  VLPEIDTALLTPLQSPAQVLPNSSKDSSQPSCVEQSTSSDENEMSDKSKKQCKSPSRRDSYSDSSGSGED
  DLVTAPANISNSTWSSALHQTLSNMVSQAQSHHEAPKSQEDTICTMFYYPQKIPNKPEFEDSNPSPLPPD
  MAPGQSYQPQSESASSSSMDKYHIHHISEPTRQENWTPLKNDLENHLEDFELEVELLITLIKSEKGSLGF
  TVTKGNQRIGCYVHDVIQDPAKSDGRLKPGDRLIKVNDTDVTNMTHTDAVNLLRAASKTVRLVIGRVLEL
  PRIPMLPHLLPDITLTCNKEELGFSLCGGHDSLYQVVYISDINPRSVAAIEGNLQLLDVIHYVNGVSTQG
  MTLEEVNRALDMSLPSLVLKATRNDLPVVPSSKRSAVSAPKSTKGNGSYSVGSCSQPALTPNDSFSTVAG
  EEINEISYPKGKCSTYQIKGSPNLTLPKESYIQEDDIYDDSQEAEVIQSLLDVVDEEAQNLLNENNAAGY
  SCGPGTLKMNGKLSEERTEDTDCDGSPLPEYFTEATKMNGCEEYCEEKVKSESLIQKPQEKKTDDDEITW
  GNDELPIERTNHEDSDKDHSFLTNDELAVLPVVKVLPSGKYTGANLKSVIRVLRGLLDQGIPSKELENLQ
  ELKPLDQCLIGQTKENRRKNRYKNILPYDATRVPLGDEGGYINASFIKIPVGKEEFVYIACQGPLPTTVG
  DFWQMIWEQKSTVIAMMTQEVEGEKIKCQRYWPNILGKTTMVSNRLRLALVRMQQLKGFVVRAMTLEDIQ
  TREVRHISHLNFTAWPDHDTPSQPDDLLTFISYMRHIHRSGPIITHCSAGIGRSGTLICIDVVLGLISQD
  LDFDISDLVRCMRLQRHGMVQTEDQYIFCYQVILYVLTRLQAEEEQKQQPQLLK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080684
• ORF Size: 6882 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080684.3
• RefSeq Size: 7546 bp
• RefSeq ORF: 6885 bp
• Locus ID: 5783
• UniProt ID: Q12923
• Cytogenetics: 4q21.3
• Domains: Y_phosphatase, B41, PDZ
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large intracellular protein. It has a catalytic PTP domain at its C-terminus and two major structural domains: a region with five PDZ domains and a FERM domain that binds to plasma membrane and cytoskeletal elements. This PTP was found to interact with, and dephosphorylate, Fas receptor and IkappaBalpha through the PDZ domains. This suggests it has a role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathways. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq, Oct 2008]