PDS5B (NM_015032) Human Tagged ORF Clone

CAT#: RG216203

PDS5B (tGFP-tagged) - Human PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) (PDS5B)

Product Images

Circular map for RG216203

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDS5B
• Synonyms: APRIN; AS3; CG008
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216203 representing NM_015032
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCATTCAAAGACTAGGACCAATGATGGAAAAATTACATATCCGCCTGGGGTCAAGGAAATATCAG
  ATAAAATATCTAAAGAGGAGATGGTGAGACGATTAAAGATGGTTGTGAAAACTTTTATGGATATGGACCA
  GGACTCTGAAGAAGAAAAGGAGCTTTATTTAAACCTAGCTTTACATCTTGCTTCAGATTTTTTTCTCAAG
  CATCCTGATAAAGATGTTCGCTTACTGGTAGCCTGCTGCCTTGCTGATATTTTCAGGATTTATGCTCCTG
  AAGCTCCTTACACATCCCCTGATAAACTAAAGGATATATTTATGTTTATAACAAGACAGTTGAAGGGGCT
  AGAGGATACAAAGAGCCCACAATTCAATAGGTATTTTTATTTACTTGAGAACATTGCTTGGGTCAAGTCA
  TATAACATATGCTTTGAGTTAGAAGATAGCAATGAAATTTTCACCCAGCTATACAGAACCTTATTTTCAG
  TTATAAACAATGGCCACAATCAGAAAGTCCATATGCACATGGTAGACCTTATGAGCTCTATTATTTGTGA
  AGGTGATACAGTGTCTCAGGAGCTTTTGGATACGGTTTTAGTAAATCTGGTACCTGCTCATAAGAATTTA
  AACAAGCAAGCATATGATTTGGCAAAGGCTTTACTGAAGAGGACAGCTCAAGCTATTGAGCCATATATTA
  CCAATTTTTTTAATCAGGTTCTGATGCTTGGGAAAACATCTATCAGCGATTTGTCAGAGCATGTCTTTGA
  CTTAATTTTGGAGCTCTACAATATTGATAGTCATTTGCTGCTCTCTGTTTTACCCCAGCTTGAATTTAAA
  TTAAAGAGCAATGATAATGAGGAGCGCCTACAAGTTGTTAAACTACTGGCAAAAATGTTTGGGGCAAAGG
  ATTCAGAATTGGCTTCTCAAAACAAGCCACTTTGGCAGTGCTACTTGGGCAGGTTTAATGATATCCATGT
  ACCAATCCGCCTGGAATGTGTGAAATTTGCTAGCCATTGTCTCATGAACCATCCTGATTTAGCAAAAGAC
  TTAACAGAGTATCTTAAAGTGAGGTCACATGACCCTGAGGAAGCTATTAGACATGATGTTATTGTGTCAA
  TAGTTACAGCTGCTAAAAAGGATATTCTTCTGGTCAATGATCACTTACTTAATTTTGTGAGAGAGAGAAC
  ATTAGACAAACGATGGAGAGTACGCAAAGAAGCCATGATGGGACTTGCCCAAATTTATAAGAAATATGCT
  TTACAGTCAGCAGCTGGAAAAGATGCTGCAAAACAGATAGCATGGATCAAAGACAAATTGCTACATATAT
  ATTATCAAAATAGTATTGATGATCGACTACTTGTTGAACGGATCTTTGCTCAATACATGGTTCCTCACAA
  TTTAGAAACTACAGAACGGATGAAATGCTTATATTACTTGTATGCCACACTGGATTTAAATGCTGTGAAA
  GCATTGAATGAAATGTGGAAATGTCAAAATCTGCTCCGACATCAAGTAAAGGATTTGCTTGACTTGATTA
  AGCAACCCAAAACAGATGCCAGTGTCAAGGCCATATTTTCAAAAGTGATGGTTATTACAAGAAATTTACC
  TGATCCTGGTAAGGCTCAGGATTTCATGAAGAAATTCACACAGGTGTTAGAAGATGATGAGAAAATAAGA
  AAGCAGTTAGAAGTACTTGTTAGTCCAACATGCTCCTGCAAGCAGGCTGAAGGTTGTGTGCGTGAAATAA
  CTAAGAAGTTGGGCAACCCCAAACAGCCTACAAATCCTTTCCTGGAAATGATCAAGTTTCTCTTGGAGAG
  GATAGCACCTGTGCACATAGATACCGAATCTATCAGTGCTCTTATTAAACAAGTGAACAAATCAATAGAT
  GGAACAGCAGATGATGAAGATGAGGGTGTTCCAACTGATCAAGCCATCAGAGCAGGTCTTGAACTGCTTA
  AGGTACTCTCATTTACACATCCCATCTCATTTCATTCTGCTGAAACATTTGAATCATTACTGGCTTGTCT
  GAAAATGGATGATGAAAAAGTAGCAGAAGCTGCACTACAAATTTTCAAAAACACAGGAAGCAAAATTGAA
  GAGGATTTTCCACACATCAGATCAGCCTTGCTTCCTGTTTTACATCACAAATCTAAAAAAGGACCCCCCC
  GTCAAGCCAAATATGCCATTCATTGTATCCATGCGATATTTTCTAGTAAAGAGACCCAGTTTGCACAGAT
  ATTTGAGCCTCTGCATAAGAGCCTAGATCCAAGCAACCTGGAACATCTCATAACACCATTGGTTACTATT
  GGTCATATTGCTCTCCTTGCACCTGATCAATTTGCTGCTCCTTTGAAATCTTTGGTAGCTACTTTCATTG
  TGAAAGATCTTCTCATGAATGATCGGCTTCCAGGGAAAAAGACAACTAAACTTTGGGTTCCAGATGAAGA
  AGTATCTCCTGAGACAATGGTCAAAATTCAGGCTATTAAAATGATGGTTCGATGGCTACTTGGAATGAAA
  AATAATCACAGTAAATCAGGAACTTCTACCTTAAGATTGCTAACAACAATATTGCATAGTGATGGAGACT
  TGACAGAACAGGGGAAAATTAGTAAACCAGATATGTCACGTCTGAGACTTGCTGCTGGGAGTGCTATTGT
  GAAGCTGGCACAAGAACCCTGTTACCATGAAATCATCACATTAGAACAATATCAGCTATGTGCATTAGCT
  ATCAACGATGAATGCTATCAAGTAAGACAAGTGTTTGCCCAGAAACTTCACAAAGGCCTTTCCCGTTTAC
  GGCTTCCACTTGAGTATATGGCAATCTGTGCCCTTTGTGCAAAAGATCCTGTAAAGGAGAGAAGAGCTCA
  TGCTAGGCAATGTTTGGTGAAAAATATAAATGTAAGGCGGGAGTATCTGAAGCAGCATGCAGCTGTTAGT
  GAAAAATTATTGTCTCTTCTACCAGAGTATGTTGTTCCATATACAATTCACCTTTTGGCACATGACCCAG
  ATTATGTCAAAGTACAGGATATTGAACAACTTAAAGATGTTAAAGAATGTCTTTGGTTTGTTCTGGAAAT
  ATTAATGGCTAAAAATGAAAATAACAGTCACGCTTTTATCAGAAAGATGGTAGAAAATATTAAACAAACA
  AAAGATGCCCAAGGACCAGATGATGCAAAAATGAATGAAAAACTGTACACTGTGTGTGATGTTGCCATGA
  ATATCATCATGTCAAAGAGTACTACATACAGTTTGGAATCTCCTAAAGACCCGGTACTACCAGCTCGTTT
  CTTCACTCAACCTGACAAGAATTTCAGTAACACCAAAAATTATCTGCCTCCTGAAATGAAATCATTTTTC
  ACTCCTGGAAAACCTAAAACAACCAATGTTCTAGGAGCTGTTAACAAGCCACTTTCATCAGCAGGCAAGC
  AATCTCAGACCAAATCATCACGAATGGAAACTGTAAGCAATGCAAGCAGCAGCTCAAATCCAAGCTCTCC
  TGGAAGAATAAAGGGGAGGCTTGATAGTTCTGAAATGGATCACAGTGAAAATGAAGATTACACAATGTCT
  TCACCTTTGCCGGGGAAAAAAAGTGACAAGAGAGACGACTCTGATCTTGTAAGGTCTGAATTGGAGAAGC
  CTAGAGGCAGGAAAAAAACGCCCGTCACAGAACAGGAGGAGAAATTAGGTATGGATGACTTGACTAAGTT
  GGTACAGGAACAGAAACCTAAAGGCAGTCAGCGAAGTCGGAAAAGAGGCCATACGGCTTCAGAATCTGAT
  GAACAGCAGTGGCCTGAGGAAAAGAGGCTCAAAGAAGATATATTAGAAAATGAAGATGAACAGAATAGTC
  CGCCAAAAAAGGGTAAAAGAGGCCGACCACCAAAACCTCTTGGTGGAGGTACACCAAAAGAAGAGCCAAC
  AATGAAAACTTCTAAAAAAGGAAGCAAAAAAAAATCTGGACCTCCAGCACCAGAGGAGGAGGAAGAAGAA
  GAAAGACAAAGTGGAAATACGGAACAGAAGTCCAAAAGCAAACAGCACCGAGTGTCAAGGAGAGCACAGC
  AGAGAGCAGAATCTCCTGAATCTAGTGCAATTGAATCCACACAGTCCACACCACAGAAAGGACGAGGAAG
  ACCATCAAAAACGCCATCACCATCACAACCAAAAAAAAATGTCCGTGTAGGACGCTCCAAACAAGCAGCT
  ACTAAGGAAAATGATTCAAGTGAAGAAGTAGATGTGTTTCAGGGTAGCTCTCCTGTCGATGATATTCCAC
  AGGAAGAAACAGAGGAGGAGGAAGTTTCTACAGTAAATGTACGGCGGCGAAGTGCTAAAAGGGAACGGCG
  A
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216203 representing NM_015032
  Red=Cloning site Green=Tags(s)
  
  MAHSKTRTNDGKITYPPGVKEISDKISKEEMVRRLKMVVKTFMDMDQDSEEEKELYLNLALHLASDFFLK
  HPDKDVRLLVACCLADIFRIYAPEAPYTSPDKLKDIFMFITRQLKGLEDTKSPQFNRYFYLLENIAWVKS
  YNICFELEDSNEIFTQLYRTLFSVINNGHNQKVHMHMVDLMSSIICEGDTVSQELLDTVLVNLVPAHKNL
  NKQAYDLAKALLKRTAQAIEPYITNFFNQVLMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQLEFK
  LKSNDNEERLQVVKLLAKMFGAKDSELASQNKPLWQCYLGRFNDIHVPIRLECVKFASHCLMNHPDLAKD
  LTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDILLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYA
  LQSAAGKDAAKQIAWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETTERMKCLYYLYATLDLNAVK
  ALNEMWKCQNLLRHQVKDLLDLIKQPKTDASVKAIFSKVMVITRNLPDPGKAQDFMKKFTQVLEDDEKIR
  KQLEVLVSPTCSCKQAEGCVREITKKLGNPKQPTNPFLEMIKFLLERIAPVHIDTESISALIKQVNKSID
  GTADDEDEGVPTDQAIRAGLELLKVLSFTHPISFHSAETFESLLACLKMDDEKVAEAALQIFKNTGSKIE
  EDFPHIRSALLPVLHHKSKKGPPRQAKYAIHCIHAIFSSKETQFAQIFEPLHKSLDPSNLEHLITPLVTI
  GHIALLAPDQFAAPLKSLVATFIVKDLLMNDRLPGKKTTKLWVPDEEVSPETMVKIQAIKMMVRWLLGMK
  NNHSKSGTSTLRLLTTILHSDGDLTEQGKISKPDMSRLRLAAGSAIVKLAQEPCYHEIITLEQYQLCALA
  INDECYQVRQVFAQKLHKGLSRLRLPLEYMAICALCAKDPVKERRAHARQCLVKNINVRREYLKQHAAVS
  EKLLSLLPEYVVPYTIHLLAHDPDYVKVQDIEQLKDVKECLWFVLEILMAKNENNSHAFIRKMVENIKQT
  KDAQGPDDAKMNEKLYTVCDVAMNIIMSKSTTYSLESPKDPVLPARFFTQPDKNFSNTKNYLPPEMKSFF
  TPGKPKTTNVLGAVNKPLSSAGKQSQTKSSRMETVSNASSSSNPSSPGRIKGRLDSSEMDHSENEDYTMS
  SPLPGKKSDKRDDSDLVRSELEKPRGRKKTPVTEQEEKLGMDDLTKLVQEQKPKGSQRSRKRGHTASESD
  EQQWPEEKRLKEDILENEDEQNSPPKKGKRGRPPKPLGGGTPKEEPTMKTSKKGSKKKSGPPAPEEEEEE
  ERQSGNTEQKSKSKQHRVSRRAQQRAESPESSAIESTQSTPQKGRGRPSKTPSPSQPKKNVRVGRSKQAA
  TKENDSSEEVDVFQGSSPVDDIPQEETEEEEVSTVNVRRRSAKRERR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015032
• ORF Size: 4341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015032.4
• RefSeq Size: 7498 bp
• RefSeq ORF: 4344 bp
• Locus ID: 23047
• UniProt ID: Q9NTI5
• Cytogenetics: 13q13.1
• Domains: AT_hook
• Gene Summary: This gene encodes a protein that interacts with the conserved protein complex termed cohesin. The cohesin complex holds together sister chromatids and facilitates accurate chromosome segregation during mitosis and meiosis. This protein is also a negative regulator of cell proliferation and may be a tumor-suppressor gene. [provided by RefSeq, Jul 2015]