NFAT5 (NM_006599) Human Tagged ORF Clone

CAT#: RG216142

NFAT5 (tGFP-tagged) - Human nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant 3

Product Images

Circular map for RG216142

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NFAT5
• Synonyms: NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216142 representing NM_006599
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCCCTCTACTCGC
  GAGAATCTGTCTATGATCTTCTCCCAAAGGAGTTACAGTTACCTCCATCTAGAGAAACATCTGTAGCATC
  AATGAGTCAGACAAGCGGTGGTGAGGCAGGCTCGCCTCCTCCAGCTGTTGTTGCTGCTGATGCTTCTTCA
  GCTCCCTCCTCTTCCTCCATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATT
  CTACCTCAGTCACCGACAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAG
  AGGGGTAAGTGAAAAGCAGTTAACCAGTAACACAGTTCAGCAGCATCCATCAACACCGAAGAGGCACACA
  GTCTTGTACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGT
  GTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAG
  CACCAGTTCCTACAATGATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCG
  GGGGTCAAACGACGAGATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTC
  ACTATGTGCTTTCTCAGCTTACCACGGACAACAAAGGCAACTCAAAAGCGGGAAATGGAACATTGGAAAA
  CCAAAAAGGAACTGGAGTAAAGAAGAGCCCTATGTTGTGTGGACAATATCCTGTTAAAAGTGAGGGAAAG
  GAGCTGAAGATAGTTGTACAACCTGAGACACAGCACCGAGCTCGGTACCTGACTGAGGGCAGCCGTGGCT
  CAGTGAAAGATAGAACACAGCAAGGCTTTCCTACAGTAAAGCTGGAAGGCCATAATGAACCTGTAGTGTT
  GCAAGTGTTTGTGGGCAACGACTCTGGACGAGTGAAACCACATGGATTTTATCAGGCCTGCAGAGTAACT
  GGACGAAATACAACTCCTTGCAAAGAAGTGGACATTGAAGGCACTACTGTTATAGAAGTCGGCCTTGATC
  CTAGCAACAACATGACACTGGCGGTGGACTGCGTAGGGATATTGAAATTGAGGAATGCTGATGTCGAAGC
  CAGAATAGGAATTGCTGGTTCCAAGAAGAAAAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCATG
  AGGAAAGATGGCTCCACTTTGACACTGCAAACACCCTCTTCTCCAATTTTGTGTACTCAGCCAGCAGGAG
  TGCCAGAAATCTTAAAGAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAAGAAGAAGTGTTTTTAATCGG
  CAAGAACTTTCTGAAAGGAACTAAAGTTATTTTCCAAGAAAATGTTTCTGATGAAAACTCTTGGAAGTCA
  GAAGCTGAAATTGATATGGAACTATTTCATCAGAATCATCTTATTGTGAAGGTTCCTCCCTATCATGACC
  AACATATAACTTTGCCTGTGTCAGTGGGAATATATGTAGTGACAAATGCTGGAAGATCTCATGATGTTCA
  ACCATTCACTTACACTCCAGACCCAGCAGCAGCTGGTGCTTTGAATGTAAATGTGAAGAAGGAAATATCT
  AGTCCAGCAAGACCTTGCTCTTTTGAAGAGGCCATGAAAGCAATGAAAACTACTGGATGTAATTTAGATA
  AGGTAAATATTATCCCTAATGCCCTGATGACTCCACTCATACCAAGCAGTATGATTAAGAGTGAAGATGT
  TACTCCAATGGAAGTAACAGCAGAAAAAAGATCTTCCACTATTTTTAAGACTACAAAGTCTGTTGGATCA
  ACTCAGCAAACATTAGAAAACATCTCAAACATAGCAGGAAATGGCTCTTTTTCATCACCATCATCTTCCC
  ACCTACCTTCTGAAAATGAAAAACAGCAGCAGATTCAGCCCAAGGCATACAACCCAGAGACCCTGACAAC
  TATTCAAACCCAGGACATCTCACAGCCTGGTACTTTTCCAGCAGTTTCTGCTTCTAGTCAGCTGCCCAAC
  AGCGATGCACTATTGCAGCAGGCTACACAGTTTCAGACAAGAGAAACTCAGTCTAGAGAGATATTACAGT
  CAGATGGTACAGTGGTTAATTTGTCACAACTGACTGAGGCATCACAACAACAGCAGCAGTCACCACTACA
  AGAACAAGCACAGACTTTACAGCAGCAGATTTCATCAAATATTTTTCCATCACCAAATAGTGTGAGTCAG
  CTTCAGAATACTATTCAGCAGCTGCAAGCAGGGAGTTTCACAGGCAGTACTGCTAGTGGCAGCAGTGGAA
  GTGTTGACTTGGTCCAACAAGTTTTAGAGGCACAGCAGCAGTTATCTTCAGTTTTATTTTCTGCTCCAGA
  TGGTAATGAGAATGTTCAAGAGCAGCTTAGTGCAGATATTTTTCAACAAGTCAGTCAAATTCAGAGTGGT
  GTAAGCCCTGGAATGTTTTCCTCAACAGAGCCAACAGTCCATACCAGACCAGATAATTTATTACCTGGAA
  GAGCTGAAAGTGTTCATCCACAGTCTGAAAACACGTTATCTAATCAACAGCAGCAGCAGCAGCAGCAACA
  GCAAGTGATGGAATCTTCAGCCGCAATGGTGATGGAGATGCAACAGAGTATCTGCCAGGCAGCTGCCCAG
  ATTCAGTCAGAGTTATTCCCTTCAACTGCTTCAGCAAATGGAAACCTTCAGCAATCGCCAGTTTACCAGC
  AGACTTCTCACATGATGAGTGCATTGTCTACCAATGAGGATATGCAAATGCAGTGTGAATTGTTTTCTTC
  TCCTCCTGCAGTTTCTGGAAATGAAACTTCTACAACTACCACACAGCAGGTTGCAACCCCTGGCACTACC
  ATGTTTCAGACATCAAGTTCAGGAGATGGAGAAGAAACTGGAACACAAGCAAAACAGATTCAGAACAGTG
  TCTTTCAGACCATGGTCCAAATGCAACATAGTGGGGACAATCAACCTCAAGTTAACCTTTTTTCATCCAC
  AAAAAGTATGATGAGTGTTCAGAATAGTGGTACCCAACAACAAGGTAATGGTTTATTCCAGCAAGGGAAT
  GAGATGATGTCACTTCAATCTGGAAATTTTTTGCAGCAGTCTTCTCATTCACAGGCCCAACTTTTTCATC
  CTCAAAATCCTATTGCCGATGCTCAGAACCTTTCCCAGGAAACTCAAGGTTCTCTCTTTCATAGTCCAAA
  TCCTATTGTCCACAGTCAGACTTCTACAACCTCCTCTGAACAAATGCAGCCTCCAATGTTTCACTCTCAA
  AGTACCATTGCTGTGTTACAGGGCTCTTCAGTTCCTCAAGACCAGCAGTCAACCAACATATTTCTTTCCC
  AGAGTCCCATGAATAATCTTCAGACTAACACAGTAGCCCAAGAAGCATTTTTTGCAGCACCGAACTCAAT
  TTCTCCACTTCAGTCAACATCAAACAGTGAACAACAAGCTGCTTTCCAACAGCAAGCTCCAATATCACAC
  ATCCAGACTCCTATGCTTTCCCAAGAACAGGCACAACCCCCGCAGCAGGGTTTATTTCAGCCTCAGGTGG
  CCCTGGGCTCCCTTCCACCTAATCCAATGCCTCAAAGCCAACAAGGAACCATGTTCCAGTCACAGCACTC
  AATAGTTGCCATGCAGAGTAACTCTCCATCCCAGGAACAGCAGCAGCAGCAGCAACAGCAGCAGCAACAG
  CAGCAGCAACAACAACAGAGCATTTTATTCAGTAATCAGAATACCATGGCTACAATGGCGTCTCCAAAGC
  AACCACCACCAAACATGATATTCAACCCAAATCAAAATCCAATGGCTAATCAGGAGCAACAGAACCAGTC
  AATTTTTCACCAACAAAGTAACATGGCCCCAATGAATCAAGAGCAACAGCCCATGCAATTTCAGAGTCAG
  TCCACAGTTTCCTCACTTCAGAACCCAGGTCCTACCCAGTCGGAATCATCACAGACCCCCTTGTTCCATA
  GCTCTCCTCAGATTCAGTTGGTACAAGGGTCACCTAGTTCTCAAGAGCAGCAAGTAACTCTCTTCTTATC
  TCCAGCATCCATGTCTGCCTTGCAGACCAGTATAAATCAACAAGATATGCAACAGTCTCCTCTTTATTCC
  CCTCAGAACAACATGCCTGGAATTCAAGGAGCCACATCTTCGCCTCAACCACAGGCTACTTTATTTCACA
  ACACAGCAGGAGGCACAATGAACCAACTGCAGAATTCTCCTGGCTCATCTCAGCAGACATCAGGAATGTT
  CTTATTTGGCATTCAAAATAACTGTAGTCAGCTTTTAACCTCTGGACCAGCTACATTGCCTGATCAGTTG
  ATGGCCATAAGTCAGCCAGGCCAACCACAAAACGAGGGCCAGCCACCTGTGACAACACTTCTTTCTCAGC
  AAATGCCAGAGAATTCTCCACTGGCATCCTCTATAAACACCAACCAGAACATCGAAAAGATTGATTTGCT
  TGTTTCATTGCAAAACCAAGGGAACAACTTGACTGGCTCCTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216142 representing NM_006599
  Red=Cloning site Green=Tags(s)
  
  MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASS
  APSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHT
  VLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRP
  GVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGK
  ELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVT
  GRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIM
  RKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKS
  EAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEIS
  SPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGS
  TQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPN
  SDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQ
  LQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSG
  VSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQ
  IQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTT
  MFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGN
  EMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQ
  STIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISH
  IQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQ
  QQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQ
  STVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYS
  PQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQL
  MAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006599
• ORF Size: 4593 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006599.4
• RefSeq Size: 13253 bp
• RefSeq ORF: 4596 bp
• Locus ID: 10725
• UniProt ID: O94916
• Cytogenetics: 16q22.1
• Domains: IPT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]