UMODL1 (NM_001004416) Human Tagged ORF Clone

CAT#: RG215569

UMODL1 (tGFP-tagged) - Human uromodulin-like 1 (UMODL1), transcript variant 1

Product Images

Circular map for RG215569

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UMODL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215569 representing NM_001004416
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAGGACCTCGGGGCTGGCACTGCTGGCTCTGGTCAGTGCTGTGGGCCCAAGCCAGGCCAGCGGCT
  TCACAGAAAAAGGCCTCTCCCTGTTGGGCTACCAGCTATGCAGCCACCGTGTGACCCACACTGTACAGAA
  GGTGGAGGCCGTGCAGACGTCCTACACGTCCTATGTGTCCTGCGGCGGCTGGATCCCCTGGAGGCGGTGC
  CCTAAGATGGTTTACCGGACACAGTACCTGGTAGTGGAGGTCCCCGAGTCCAGGAACGTGACTGACTGCT
  GTGAGGGCTATGAACAGCTCGGCCTCTACTGTGTCTTGCCCCTGAATCAGTCCGGGCAGTTCACGTCAAG
  ACCTGGGGCCTGCCCCGCAGAGGGGCCTGAACCATCCACCTCCCCCTGCAGCTTGGACATCGACTGTCCT
  GGACTTGAGAAGTGCTGCCCCTGGTCAGGGGGGCGCTACTGCATGGCCCCTGCACCCCAAGCTCCAGAGA
  GGGACCCTGTGGGCTCCTGGTACAACGTCACCATACTGGTGAAAATGGACTTCAAGGAACTCCAGCAAGT
  GGACCCCAGGCTCCTGAACCACATGCGCCTTCTGCATTCCTTGGTCACCAGCGCCCTGCAACCAATGGCC
  TCCACCGTCCACCACCTGCACTCAGCCCCTGGGAACGCCTCCACCACAGTGTCGCGGCTGCTACTGGGCC
  TGCCACGGCCACTGCCTGTGGCTGACGTCTCCACCCTGCTGGGTGACATTGCGAAGCGTGTCTATGAAGT
  GATCAGCGTCCAGGTGCAAGATGTCAATGAGTGTTTCTATGAGGAGCTCAATGCCTGCTCTGGAAGGGAA
  CTGTGCGCAAACCTGGAGGGCTCGTACTGGTGCGTCTGTCACCAGGAAGCTCCAGCCACGTCTCCACGGA
  AGCTGAACCTGGAGTGGGAAGATTGTCCTCCAGTCAGTGACTACGTGGTCCTCAACGTCACCAGTGACAG
  TTTTCAAGTATCCTGGCGTTTAAATTCTACACAGAACCACACTTTCCATGTCCGGGTTTACCGGGGTATG
  GAGTTGCTCAGGAGCGCCAGGACACAGAGCCAGGCACTGGCAGTGGCTGGGCTGGAGGCTGGAGTGCTGT
  ACAGGGTGAAGACCAGCTACCAGGGGTGCGGGGCCGACGTCTCCACCACGCTGACCATCAAAACCAATGC
  CCAGGTATTTGAAGTCACAATAAAGATTGTAAACCACAACCTGACGGAGAAGTTACTCAACCGCAGCAGT
  GTGGAGTACCAGGACTTTTCTAGACAACTGCTTCACGAGGTCGAGAGCTCCTTCCCACCAGTGGTGTCTG
  ACTTGTACCGAAGTGGGAAGCTGAGAATGCAGATCGTGTCTCTCCAGGCGGGAAGTGTGGTCGTGAGGCT
  CAAGCTCACCGTGCAGGACCCCGGGTTTCCCATGGGCATCTCCACGCTGGCCCCCATACTCCAGCCCCTG
  TTGGCAAGCACAGTGTTCCAGATTGACCGGCAGGGGACACGCGTGCAAGACTGGGACGAGTGTGTGGACA
  GCGCGGAACACGACTGCTCACCGGCTGCCTGGTGCATCAACCTGGAGGGCTCCTACACCTGCCAGTGCCG
  TACCACCAGGGACGCCACCCCCTCCCGCGCAGGCCGGGCCTGTGAGGGTGACCTGGTGAGCCCCACGGGC
  GGTGGACTGTCTGCGGCAACAGGGGTAACGGTCCCAGGTCTTGGCACGGGAACAGCAGCCCTCGGCCTAG
  AGAACTTCACCTTGTCACCCAGTCCTGGGTACCCTCAGGGCACCCCGGCAGCAGGCCAGGCCTGGACCCC
  AGAGCCCTCACCCAGAAGAGGGGGCAGCAATGTGGTCGGGTATGACAGGAACAACACAGGAAAAGGCGTG
  GAGCAGGAGCTACAGGGAAACTCCATCATGGAGCCACCCTCCTGGCCTTCCCCTACTGAGGACCCCACCG
  GCCACTTCCTGTGGCATGCCACCCGTTCCACCCGGGAAACACTTCTGAATCCCACGTGGCTGCGAAATGA
  GGACAGTGGACCCTCCGGTTCTGTAGACCTGCCATTGACCTCCACCCTCACAGCTCTGAAGACCCCCGCC
  TGTGTTCCTGTCTCCATTGGGAGGATCATGGTCTCCAATGTGACCAGCACCGGCTTCCACCTGGCATGGG
  AGGCGGATCTTGCTATGGACTCCACCTTCCAGCTCACTCTGACTTCCATGTGGAGCCCTGCTGTGGTCCT
  AGAGACCTGGAACACGAGTGTGACACTGTCGGGGCTGGAGCCTGGGGTCTTGCACCTGGTTGAGATCATG
  GCCAAAGCATGTGGGAAAGAAGGTGCCAGAGCTCATCTGAAAGTGAGGACAGCAGCCCGGAAGCTCATTG
  GAAAGGTCAGAATCAAAAATGTCAGGTACTCAGAATCCTTTCGCAACGCAAGCAGCCAGGAGTATCGAGA
  TTTCCTAGAACTATTCTTCAGGATGGTGCGGGGCTCCCTGCCAGCCACCATGTGTCAGCACATGGACGCT
  GGTGGGGTCAGGATGGAAGTCGTCAGCGTCACCAACGGCAGCATCGTGGTGGAGTTTCACTTGCTGATAA
  TCGCAGATGTGGATGTCCAGGAGGTGTCAGCTGCATTTCTCACCGCCTTCCAGACCGTGCCTCTGCTGGA
  GGTGATCAGAGGCGACACCTTCATACAGGATTACGATGAGTGTGAAAGGAAGGAGGACGACTGTGTGCCG
  GGGACATCCTGTCGAAACACCCTCGGGTCTTTCACTTGTAGCTGCGAGGGAGGAGCCCCCGACTTCCCTG
  TGGAATATTCTGAGAGACCCTGTGAAGGTGACTCTCCTGGCAATGAAACCTGGGCCACCAGCCCAGAGAG
  GCCTCTCACCACAGCAGGGACCAAGGCTGCCTTTGTGCAAGGCACCAGCCCCACCCCCCAAGGCCTGCCC
  CAGCGGCTGAACCTGACCGGAGCAGTCAGGGTGCTCTGTGAGATCGAGAAGGTGGTTGTCGCCATCCAGA
  AGCGCTTCCTGCAGCAGGAATCCATCCCCGAGTCCTCGTTGTACCTCAGCCACCCCTCCTGCAACGTGAG
  CCACAGCAATGGCACACACGTGCTCCTGGAGGCCGGCTGGAGCGAGTGTGGGACCCTCATGCAGAGCAAC
  ATGACGAACACCGTGGTGAGGACCACGCTGAGGAACGACCTGTCCCAGGAGGGCATCATCCACCACCTGA
  AGATCCTGAGCCCCATCTACTGCGCCTTCCAGAATGACCTGCTGACATCCTCCGGCTTCACCCTGGAGTG
  GGGGGTTTACACCATCATCGAGGACCTCCACGGCGCTGGGAATTTTGTTACCGAAATGCAGTTGTTTATC
  GGAGACTCTCCCATACCTCAGAATTATAGCGTGTCTGCCAGTGACGATGTCAGGATCGAAGTGGGGCTCT
  ACAGGCAGAAAAGCAACCTCAAGGTGGTCCTGACGGAGTGCTGGGCAACCCCGTCTAGCAACGCCCGGGA
  CCCCATCACCTTCAGCTTCATTAACAACAGCTGCCCTGTGCCCAACACATACACCAACGTGATTGAGAAC
  GGCAACTCCAATAAGGCCCAGTTCAAGCTGAGGATCTTTTCCTTTATCAACGACTCCATCGTCTACCTGC
  ACTGCAAACTCCGCGTCTGCATGGAATCCCCCGGAGCCACGTGCAAAATCAATTGCAATAACTTTCGGTT
  GCTGCAAAATAGTGAAACCTCTGCCACACACCAGATGTCCTGGGGACCCCTCATCCGGTCTGAAGGTGAG
  CCTCCTCATGCAGAAGCAGGCCTGGGTGCCGGTTATGTGGTCCTTATTGTGGTGGCCATCTTCGTGCTGG
  TGGCGGGAACAGCCACCCTTCTGATCGTGCGCTACCAGAGAATGAATGGGAGATACAACTTTAAAATCCA
  GTCCAACAACTTCAGCTACCAGGTGTTCTACGAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215569 representing NM_001004416
  Red=Cloning site Green=Tags(s)
  
  MLRTSGLALLALVSAVGPSQASGFTEKGLSLLGYQLCSHRVTHTVQKVEAVQTSYTSYVSCGGWIPWRRC
  PKMVYRTQYLVVEVPESRNVTDCCEGYEQLGLYCVLPLNQSGQFTSRPGACPAEGPEPSTSPCSLDIDCP
  GLEKCCPWSGGRYCMAPAPQAPERDPVGSWYNVTILVKMDFKELQQVDPRLLNHMRLLHSLVTSALQPMA
  STVHHLHSAPGNASTTVSRLLLGLPRPLPVADVSTLLGDIAKRVYEVISVQVQDVNECFYEELNACSGRE
  LCANLEGSYWCVCHQEAPATSPRKLNLEWEDCPPVSDYVVLNVTSDSFQVSWRLNSTQNHTFHVRVYRGM
  ELLRSARTQSQALAVAGLEAGVLYRVKTSYQGCGADVSTTLTIKTNAQVFEVTIKIVNHNLTEKLLNRSS
  VEYQDFSRQLLHEVESSFPPVVSDLYRSGKLRMQIVSLQAGSVVVRLKLTVQDPGFPMGISTLAPILQPL
  LASTVFQIDRQGTRVQDWDECVDSAEHDCSPAAWCINLEGSYTCQCRTTRDATPSRAGRACEGDLVSPTG
  GGLSAATGVTVPGLGTGTAALGLENFTLSPSPGYPQGTPAAGQAWTPEPSPRRGGSNVVGYDRNNTGKGV
  EQELQGNSIMEPPSWPSPTEDPTGHFLWHATRSTRETLLNPTWLRNEDSGPSGSVDLPLTSTLTALKTPA
  CVPVSIGRIMVSNVTSTGFHLAWEADLAMDSTFQLTLTSMWSPAVVLETWNTSVTLSGLEPGVLHLVEIM
  AKACGKEGARAHLKVRTAARKLIGKVRIKNVRYSESFRNASSQEYRDFLELFFRMVRGSLPATMCQHMDA
  GGVRMEVVSVTNGSIVVEFHLLIIADVDVQEVSAAFLTAFQTVPLLEVIRGDTFIQDYDECERKEDDCVP
  GTSCRNTLGSFTCSCEGGAPDFPVEYSERPCEGDSPGNETWATSPERPLTTAGTKAAFVQGTSPTPQGLP
  QRLNLTGAVRVLCEIEKVVVAIQKRFLQQESIPESSLYLSHPSCNVSHSNGTHVLLEAGWSECGTLMQSN
  MTNTVVRTTLRNDLSQEGIIHHLKILSPIYCAFQNDLLTSSGFTLEWGVYTIIEDLHGAGNFVTEMQLFI
  GDSPIPQNYSVSASDDVRIEVGLYRQKSNLKVVLTECWATPSSNARDPITFSFINNSCPVPNTYTNVIEN
  GNSNKAQFKLRIFSFINDSIVYLHCKLRVCMESPGATCKINCNNFRLLQNSETSATHQMSWGPLIRSEGE
  PPHAEAGLGAGYVVLIVVAIFVLVAGTATLLIVRYQRMNGRYNFKIQSNNFSYQVFYE
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004416
• ORF Size: 3954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004416.2, NP_001004416.2
• RefSeq Size: 4878 bp
• RefSeq ORF: 3957 bp
• Locus ID: 89766
• UniProt ID: Q5DID0
• Cytogenetics: 21q22.3
• Protein Families: Transmembrane