TRAF7 (NM_032271) Human Tagged ORF Clone

CAT#: RG215162

TRAF7 (tGFP-tagged) - Human TNF receptor-associated factor 7 (TRAF7)

Product Images

Circular map for RG215162

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRAF7
• Synonyms: CAFDADD; RFWD1; RNF119
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215162 representing NM_032271
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCAGGCAAGAGTGCCCGCTACAACCGCTTCTCCGGGGGGCCCAGCAATCTTCCCACCCCAGACG
  TCACCACAGGGACCAGAATGGAAACGACCTTCGGACCCGCCTTTTCAGCCGTCACCACCATCACAAAAGC
  TGACGGGACCAGCACCTACAAGCAGCACTGCAGGACACCCTCCTCCTCCAGCACCCTTGCCTACTCCCCG
  CGGGACGAGGAGGACAGCATGCCCCCCATCAGCACTCCCCGCCGCTCCGACTCCGCCATCTCTGTCCGCT
  CCCTGCACTCAGAGTCCAGCATGTCTCTGCGCTCCACATTCTCACTGCCCGAGGAGGAGGAGGAGCCGGA
  GCCACTGGTGTTTGCGGAGCAGCCCTCGGTGAAGCTGTGCTGTCAGCTCTGCTGCAGCGTCTTCAAAGAC
  CCCGTGATCACCACGTGTGGGCACACGTTCTGTAGGAGATGCGCCTTGAAGTCAGAGAAGTGTCCCGTGG
  ACAACGTCAAACTGACCGTGGTGGTGAACAACATCGCGGTGGCCGAGCAGATCGGGGAGCTCTTCATCCA
  CTGCCGGCACGGCTGCCGGGTAGCGGGCAGCGGGAAGCCCCCCATCTTTGAGGTGGACCCCCGAGGGTGC
  CCCTTCACCATCAAGCTCAGCGCCCGGAAGGACCACGAGGGCAGCTGTGACTACAGGCCTGTGCGGTGTC
  CCAACAACCCCAGCTGCCCCCCGCTGCTCAGGATGAACCTGGAGGCCCACCTCAAGGAGTGCGAGCACAT
  CAAATGCCCCCACTCCAAGTACGGGTGCACGTTCATCGGGAACCAGGACACTTACGAGACCCACCTGGAG
  ACTTGCCGCTTCGAGGGCCTGAAGGAGTTTCTGCAGCAGACGGATGACCGCTTCCACGAGATGCACGTGG
  CTCTGGCCCAGAAGGACCAGGAGATCGCCTTCCTGCGCTCCATGCTGGGAAAGCTCTCGGAGAAGATCGA
  CCAGCTAGAGAAGAGCCTGGAGCTCAAGTTTGACGTCCTGGACGAAAACCAGAGCAAGCTCAGCGAGGAC
  CTCATGGAGTTCCGGCGGGACGCATCCATGTTAAATGACGAGCTGTCCCACATCAACGCGCGGCTGAACA
  TGGGCATCCTAGGCTCCTACGACCCTCAGCAGATCTTCAAGTGCAAAGGGACCTTTGTGGGCCACCAGGG
  CCCTGTGTGGTGTCTCTGCGTCTACTCCATGGGTGACCTGCTCTTCAGTGGCTCCTCTGACAAGACCATC
  AAGGTGTGGGACACATGTACCACCTACAAGTGTCAGAAGACACTGGAGGGCCATGATGGCATCGTGCTGG
  CTCTCTGCATCCAGGGGTGCAAACTCTACAGCGGCTCTGCAGACTGCACCATCATTGTGTGGGACATCCA
  GAACCTGCAGAAGGTGAACACCATCCGGGCCCATGACAACCCGGTGTGCACGCTGGTCTCCTCACACAAC
  GTGCTCTTCAGCGGCTCCCTGAAGGCCATCAAGGTCTGGGACATCGTGGGCACTGAGCTGAAGTTGAAGA
  AGGAGCTCACAGGCCTCAACCACTGGGTGCGGGCCCTGGTGGCTGCCCAGAGCTACCTGTACAGCGGCTC
  CTACCAGACAATCAAGATCTGGGACATCCGAACCCTTGACTGCATCCACGTCCTGCAGACGTCTGGTGGC
  AGCGTCTACTCCATTGCTGTGACAAATCACCACATTGTCTGTGGCACCTACGAGAACCTCATCCACGTGT
  GGGACATTGAGTCCAAGGAGCAGGTGCGGACCCTCACGGGCCACGTGGGCACCGTGTATGCCCTGGCGGT
  CATCTCGACGCCAGACCAGACCAAAGTCTTCAGTGCATCCTACGACCGGTCCCTCAGGGTCTGGAGTATG
  GACAACATGATCTGCACGCAGACCCTGCTGCGTCACCAGGGCAGTGTCACCGCGCTGGCTGTGTCCCGGG
  GCCGACTCTTCTCAGGGGCTGTGGATAGCACTGTGAAGGTTTGGACTTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215162 representing NM_032271
  Red=Cloning site Green=Tags(s)
  
  MSSGKSARYNRFSGGPSNLPTPDVTTGTRMETTFGPAFSAVTTITKADGTSTYKQHCRTPSSSSTLAYSP
  RDEEDSMPPISTPRRSDSAISVRSLHSESSMSLRSTFSLPEEEEEPEPLVFAEQPSVKLCCQLCCSVFKD
  PVITTCGHTFCRRCALKSEKCPVDNVKLTVVVNNIAVAEQIGELFIHCRHGCRVAGSGKPPIFEVDPRGC
  PFTIKLSARKDHEGSCDYRPVRCPNNPSCPPLLRMNLEAHLKECEHIKCPHSKYGCTFIGNQDTYETHLE
  TCRFEGLKEFLQQTDDRFHEMHVALAQKDQEIAFLRSMLGKLSEKIDQLEKSLELKFDVLDENQSKLSED
  LMEFRRDASMLNDELSHINARLNMGILGSYDPQQIFKCKGTFVGHQGPVWCLCVYSMGDLLFSGSSDKTI
  KVWDTCTTYKCQKTLEGHDGIVLALCIQGCKLYSGSADCTIIVWDIQNLQKVNTIRAHDNPVCTLVSSHN
  VLFSGSLKAIKVWDIVGTELKLKKELTGLNHWVRALVAAQSYLYSGSYQTIKIWDIRTLDCIHVLQTSGG
  SVYSIAVTNHHIVCGTYENLIHVWDIESKEQVRTLTGHVGTVYALAVISTPDQTKVFSASYDRSLRVWSM
  DNMICTQTLLRHQGSVTALAVSRGRLFSGAVDSTVKVWTC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032271
• ORF Size: 2010 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032271.3
• RefSeq Size: 3676 bp
• RefSeq ORF: 2013 bp
• Locus ID: 84231
• UniProt ID: Q6Q0C0
• Cytogenetics: 16p13.3
• Domains: zf-TRAF, WD40, RING
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.[supplied by OMIM, Apr 2004]