NLRP13 (NM_176810) Human Tagged ORF Clone

CAT#: RG215145

NLRP13 (tGFP-tagged) - Human NLR family, pyrin domain containing 13 (NLRP13)

Product Images

Circular map for RG215145

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NLRP13
• Synonyms: CLR19.7; NALP13; NOD14; PAN13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215145 representing NM_176810
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTTCTGTAATCACCTGCCCCAACGGTGGTACCAACCAAGGGCTTCTGCCTTACCTGATGGCCC
  TGGATCAGTATCAGCTGGAGGAATTCAAGCTTTGCTTGGAACCCCAGCAGCTGATGGACTTCTGGTCGGC
  CCCCCAGGGGCACTTCCCGCGTATCCCCTGGGCAAACTTGAGAGCTGCCGACCCTTTGAATCTGTCCTTT
  CTTTTGGATGAACACTTCCCAAAAGGTCAGGCATGGAAAGTGGTCCTCGGCATCTTCCAGACAATGAATC
  TGACCTCACTGTGTGAGAAAGTTAGAGCCGAGATGAAAGAGAATGTGCAGACCCAAGAGCTGCAAGATCC
  AACCCAGGAAGATCTAGAGATGCTAGAAGCAGCAGCAGGGAATATGCAGACCCAGGGATGCCAAGATCCA
  AACCAAGAAGAACTAGACGAGCTAGAAGAAGAAACAGGGAATGTACAGGCCCAGGGATGCCAAGATCCAA
  ACCAAGAAGAACCAGAGATGCTAGAGGAAGCAGACCACAGAAGAAAATACAGAGAGAACATGAAGGCTGA
  ACTACTGGAGACATGGGACAACATCAGTTGGCCTAAAGACCACGTATATATCCGTAATACATCAAAGGAC
  GAACATGAGGAACTGCAGCGCCTACTGGATCCTAATAGGACTAGAGCCCAGGCCCAGACGATAGTCTTGG
  TGGGGAGGGCAGGGGTTGGGAAGACCACCTTGGCAATGCAGGCTATGCTGCACTGGGCAAATGGAGTTCT
  CTTTCAGCAAAGGTTCTCCTATGTTTTCTATCTCAGCTGCCATAAAATAAGGTACATGAAGGAAACTACC
  TTTGCTGAATTGATTTCTTTGGATTGGCCCGATTTTGATGCCCCCATTGAAGAGTTCATGTCTCAACCAG
  AGAAGCTCCTGTTTATTATTGATGGCTTTGAGGAAATAATCATATCTGAGTCACGCTCTGAGAGCTTGGA
  TGATGGCTCGCCATGTACAGACTGGTACCAGGAGCTCCCAGTGACCAAAATCCTACACAGCTTGTTGAAG
  AAAGAATTGGTTCCCCTGGCTACCTTACTGATCACGATCAAGACCTGGTTTGTGAGAGATCTTAAGGCCT
  CATTAGTGAATCCATGCTTTGTACAAATTACAGGGTTCACAGGGGACGACCTACGGGTATATTTCATGAG
  ACACTTTGATGACTCAAGTGAAGTTGAGAAAATCCTGCAGCAGCTAAGAAAAAACGAAACTCTCTTTCAT
  TCCTGCAGTGCCCCCATGGTGTGTTGGACCGTATGTTCCTGTCTGAAGCAGCCGAAGGTGAGGTATTACG
  ATCTCCAGTCAATCACTCAGACTACCACCAGTCTGTATGCCTATTTTTTCTCCAACTTGTTCTCCACAGC
  AGAGGTAGATTTGGCAGATGACAGCTGGCCAGGACAATGGAGGGCCCTCTGCAGCCTGGCCATAGAAGGG
  CTGTGGTCTATGAACTTCACATTTAACAAAGAAGACACTGAGATTGAGGGCCTGGAAGTGCCTTTCATTG
  ATTCTCTCTACGAGTTCAATATTCTTCAAAAGATCAATGACTGTGGGGGTTGCACTACTTTCACCCACCT
  AAGTTTCCAGGAGTTTTTTGCAGCCATGTCCTTTGTGCTAGAGGAACCTAGAGAATTCCCTCCCCATTCC
  ACAAAGCCACAAGAGATGAAGATGTTACTGCAACACGTCTTGCTTGACAAAGAAGCCTACTGGACTCCAG
  TGGTTCTGTTCTTCTTTGGTCTTTTAAATAAAAACATAGCAAGAGAACTGGAAGATACTTTGCATTGTAA
  AATATCTCCCAGGGTAATGGAGGAATTATTAAAGTGGGGAGAAGAGTTAGGTAAGGCTGAAAGTGCCTCT
  CTCCAATTTCACATTCTACGACTTTTTCACTGCCTACACGAGTCCCAGGAGGAAGACTTCACAAAGAAGA
  TGTTGGGTCGTATCTTTGAAGTTGACCTTAATATTTTGGAGGACGAAGAACTCCAAGCTTCTTCATTTTG
  CCTAAAGCACTGTAAAAGGTTAAATAAGCTAAGGCTTTCTGTTAGCAGTCACATCCTTGAAAGGGACTTG
  GAAATTCTGGAGACAAGCAAGTTTGATTCCAGGATGCACGCATGGAACAGCATTTGCTCTACGTTGGTCA
  CAAATGAGAATCTGCATGAGCTAGACCTGAGTAACAGCAAACTTCATGCTTCCTCTGTGAAGGGTCTCTG
  TCTTGCACTGAAAAATCCAAGATGCAAAGTCCAGAAACTGACGTGCAAATCGGTAACTCCTGAGTGGGTT
  CTGCAGGACCTCATTATTGCCCTTCAGGGTAACAGCAAGCTGACCCATCTGAACTTCAGCTCTAACAAGC
  TGGGAATGACTGTCCCCCTGATTCTTAAAGCTTTGAGACACTCAGCTTGCAACCTCAAGTATCTGTGCCT
  GGAGAAATGCAACTTGTCGGCAGCCAGCTGTCAGGACCTAGCCTTGTTTCTCACCAGCATCCAACACGTA
  ACTCGATTGTGCCTGGGATTTAATCGGCTCCAAGATGATGGCATAAAGCTATTGTGTGCGGCCCTGACTC
  ACCCCAAGTGTGCCTTAGAGAGACTGGAGCTCTGGTTTTGCCAGCTGGCAGCACCCGCTTGCAAGCACTT
  GTCAGATGCTCTCCTGCAGAACAGGAGCCTGACACACCTGAATCTGAGCAAGAACAGCCTGAGAGACGAG
  GGAGTCAAGTTCCTGTGTGAGGCCTTGGGTCGCCCAGATGGTAACCTGCAGAGCCTGAATTTGTCAGGTT
  GTTCTTTCACAAGAGAGGGCTGTGGAGAGCTGGCTAATGCCCTCAGCCATAATCATAATGTGAAAATCTT
  AGATTTGGGAGAAAATGATCTTCAGGATGATGGAGTGAAGCTACTGTGTGAGGCTCTGAAACCACATCGT
  GCATTGCACACACTTGGGTTGGCGAAATGCAATCTGACAACTGCTTGCTGCCAGCATCTCTTCTCTGTTC
  TCAGCAGCAGTAAGAGCCTGGTCAATCTGAACCTTCTAGGCAATGAATTGGATACTGATGGTGTCAAGAT
  GCTATGTAAGGCTTTGAAAAAGTCGACATGCAGGCTGCAGAAACTCGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215145 representing NM_176810
  Red=Cloning site Green=Tags(s)
  
  MNFSVITCPNGGTNQGLLPYLMALDQYQLEEFKLCLEPQQLMDFWSAPQGHFPRIPWANLRAADPLNLSF
  LLDEHFPKGQAWKVVLGIFQTMNLTSLCEKVRAEMKENVQTQELQDPTQEDLEMLEAAAGNMQTQGCQDP
  NQEELDELEEETGNVQAQGCQDPNQEEPEMLEEADHRRKYRENMKAELLETWDNISWPKDHVYIRNTSKD
  EHEELQRLLDPNRTRAQAQTIVLVGRAGVGKTTLAMQAMLHWANGVLFQQRFSYVFYLSCHKIRYMKETT
  FAELISLDWPDFDAPIEEFMSQPEKLLFIIDGFEEIIISESRSESLDDGSPCTDWYQELPVTKILHSLLK
  KELVPLATLLITIKTWFVRDLKASLVNPCFVQITGFTGDDLRVYFMRHFDDSSEVEKILQQLRKNETLFH
  SCSAPMVCWTVCSCLKQPKVRYYDLQSITQTTTSLYAYFFSNLFSTAEVDLADDSWPGQWRALCSLAIEG
  LWSMNFTFNKEDTEIEGLEVPFIDSLYEFNILQKINDCGGCTTFTHLSFQEFFAAMSFVLEEPREFPPHS
  TKPQEMKMLLQHVLLDKEAYWTPVVLFFFGLLNKNIARELEDTLHCKISPRVMEELLKWGEELGKAESAS
  LQFHILRLFHCLHESQEEDFTKKMLGRIFEVDLNILEDEELQASSFCLKHCKRLNKLRLSVSSHILERDL
  EILETSKFDSRMHAWNSICSTLVTNENLHELDLSNSKLHASSVKGLCLALKNPRCKVQKLTCKSVTPEWV
  LQDLIIALQGNSKLTHLNFSSNKLGMTVPLILKALRHSACNLKYLCLEKCNLSAASCQDLALFLTSIQHV
  TRLCLGFNRLQDDGIKLLCAALTHPKCALERLELWFCQLAAPACKHLSDALLQNRSLTHLNLSKNSLRDE
  GVKFLCEALGRPDGNLQSLNLSGCSFTREGCGELANALSHNHNVKILDLGENDLQDDGVKLLCEALKPHR
  ALHTLGLAKCNLTTACCQHLFSVLSSSKSLVNLNLLGNELDTDGVKMLCKALKKSTCRLQKLG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176810
• ORF Size: 3129 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176810.1, NP_789780.1
• RefSeq Size: 3132 bp
• RefSeq ORF: 3132 bp
• Locus ID: 126204
• UniProt ID: Q86W25
• Cytogenetics: 19q13.43
• Gene Summary: This gene encodes a member of the NACHT, leucine rich repeat, and PYD containing (NLRP) protein family. It has an N-terminal pyrin domain, followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. NLRP proteins are implicated in the activation of proinflammatory caspases through multiprotein complexes called inflammasomes. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]