CABYR (NM_012189) Human Tagged ORF Clone

CAT#: RG214772

CABYR (tGFP-tagged) - Human calcium binding tyrosine-(Y)-phosphorylation regulated (CABYR), transcript variant 1

Product Images

Circular map for RG214772

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CABYR
• Synonyms: CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86; CT88; FSP-2; FSP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214772 representing NM_012189
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTCTTCAAAGCCCAGACTTGTCGTACCCTATGGCCTCAAGACTCTGCTCGAGGGAATTAGCAGAG
  CTGTTCTCAAAACCAACCCATCAAACATCAACCAGTTTGCAGCAGCTTATTTTCAAGAACTTACTATGTA
  TAGAGGGAATACTACTATGGATATAAAAGATCTGGTTAAACAATTTCATCAGATTAAAGTAGAGAAATGG
  TCAGAAGGAACGACACCACAGAAGAAATTAGAATGTTTAAAAGAACCAGGAAAAACATCTGTAGAATCTA
  AAGTACCTACCCAGATGGAAAAATCTACAGACACAGACGAGGACAATGTAACCAGAACAGAATATAGTGA
  CAAAACCACCCAGTTTCCATCAGTTTATGCTGTGCCAGGCACTGAGCAAACGGAAGCAGTTGGTGGTCTT
  TCTTCCAAACCAGCCACCCCTAAGACTACTACCCCACCCTCATCACCACCTCCAACAGCTGTCTCACCAG
  AGTTTGCCTACGTCCCAGCTGACCCAGCTCAGCTTGCTGCTCAGATGTTAGGTAAAGTTTCATCTATTCA
  TTCTGATCAATCTGATGTGTTAATGGTGGATGTGGCAACCAGTATGCCTGTTGTTATCAAGGAGGTGCCA
  AGCTCAGAGGCTGCTGAAGATGTCATGGTGGCTGCTCCTCTTGTGTGTTCTGGAAAGGTGCTAGAAGTGC
  AGGTTGTGAACCAAACATCTGTCCATGTAGATTTGGGTTCTCAACCTAAAGAAAATGAGGCTGAACCATC
  AACGGCTTCCTCAGTCCCCTTGCAGGATGAACAAGAACCTCCTGCTTATGATCAAGCTCCTGAGGTCACT
  TTGCAGGCTGATATTGAGGTTATGTCAACTGTTCATATATCATCTGTCTATAACGATGTGCCTGTGACTG
  AAGGAGTTGTTTATATCGAGCAACTGCCAGAACAAATAGTTATCCCTTTTACTGATCAAGTTGCTTGTCT
  TAAAGAAAATGAGCAGTCAAAAGAAAATGAGCAGTCACCACGAGTTAGTCCCAAATCTGTAGTAGAAAAG
  ACCACCTCTGGCATGTCTAAAAAATCTGTAGAGTCTGTAAAACTTGCACAGTTGGAGGAGAATGCAAAAT
  ATTCCTCAGTATATATGGAGGCAGAAGCAACAGCTCTGCTCTCTGACACATCTTTGAAAGGTCAGCCTGA
  GGTACCTGCACAACTCCTGGATGCAGAAGGTGCTATCAAAATAGGCTCTGAAAAATCTCTGCACCTTGAA
  GTGGAGATCACTTCAATAGTCTCTGACAATACTGGGCAGGAGGAGTCTGGGGAAAACTCTGTACCCCAGG
  AGATGGAAGGCAAACCTGTGCTCTCTGGGGAAGCTGCAGAAGCAGTGCACTCAGGTACATCTGTAAAGTC
  ATCTAGTGGCCCCTTCCCTCCTGCTCCAGAAGGCCTTACTGCACCAGAAATTGAACCAGAAGGGGAATCA
  ACAGCTGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214772 representing NM_012189
  Red=Cloning site Green=Tags(s)
  
  MISSKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMDIKDLVKQFHQIKVEKW
  SEGTTPQKKLECLKEPGKTSVESKVPTQMEKSTDTDEDNVTRTEYSDKTTQFPSVYAVPGTEQTEAVGGL
  SSKPATPKTTTPPSSPPPTAVSPEFAYVPADPAQLAAQMLGKVSSIHSDQSDVLMVDVATSMPVVIKEVP
  SSEAAEDVMVAAPLVCSGKVLEVQVVNQTSVHVDLGSQPKENEAEPSTASSVPLQDEQEPPAYDQAPEVT
  LQADIEVMSTVHISSVYNDVPVTEGVVYIEQLPEQIVIPFTDQVACLKENEQSKENEQSPRVSPKSVVEK
  TTSGMSKKSVESVKLAQLEENAKYSSVYMEAEATALLSDTSLKGQPEVPAQLLDAEGAIKIGSEKSLHLE
  VEITSIVSDNTGQEESGENSVPQEMEGKPVLSGEAAEAVHSGTSVKSSSGPFPPAPEGLTAPEIEPEGES
  TAE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012189
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012189.4
• RefSeq Size: 2333 bp
• RefSeq ORF: 1482 bp
• Locus ID: 26256
• UniProt ID: O75952
• Cytogenetics: 18q11.2
• Domains: RIIa
• Gene Summary: To reach fertilization competence, spermatozoa undergo a series of morphological and molecular maturational processes, termed capacitation, involving protein tyrosine phosphorylation and increased intracellular calcium. The protein encoded by this gene localizes to the principal piece of the sperm flagellum in association with the fibrous sheath and exhibits calcium-binding when phosphorylated during capacitation. A pseudogene on chromosome 3 has been identified for this gene. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2013]