ASPM (NM_018136) Human Tagged ORF Clone

CAT#: RG214770

ASPM (tGFP-tagged) - Human asp (abnormal spindle) homolog, microcephaly associated (Drosophila) (ASPM), transcript variant 1

Product Images

Circular map for RG214770

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASPM
• Synonyms: ASP; Calmbp1; MCPH5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214770 representing NM_018136
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACCGGCGAGTGGGGCGAGGCTGCTGGGAAGTGAGCCCGACCGAGCGGAGGCCGCCCGCGGGGC
  TGCGGGGCCCCGCGGCCGAGGAGGAGGCGTCTTCCCCGCCGGTCCTGTCTCTCAGCCACTTCTGCAGGTC
  TCCTTTCCTTTGCTTCGGGGACGTTCTCCTGGGAGCCTCACGGACGCTGTCTCTGGCCCTAGACAACCCT
  AACGAGGAGGTGGCAGAAGTGAAGATCTCCCACTTCCCGGCCGCGGACCTGGGCTTCAGTGTGTCGCAGC
  GCTGTTTCGTGTTGCAGCCTAAAGAGAAAATTGTTATTTCTGTTAACTGGACACCACTCAAAGAAGGCCG
  AGTAAGAGAGATTATGACATTTCTTGTAAATGATGTTCTGAAACACCAAGCTATATTACTAGGAAATGCA
  GAAGAGCAGAAAAAGAAAAAGAGGAGTCTTTGGGATACCATTAAAAAGAAGAAAATTTCAGCCTCTACAA
  GTCACAACAGAAGGGTTTCAAATATTCAGAATGTTAATAAAACATTTAGTGTTTCCCAAAAAGTTGACAG
  AGTTAGGAGCCCACTACAAGCTTGTGAAAACTTGGCTATGAATGAAGGCGGTCCCCCAACAGAAAACAAT
  TCTTTAATACTTGAAGAAAATAAAATACCCATATCACCTATTAGCCCTGCTTTCAATGAATGCCATGGTG
  CAACTTGCTTGCCACTCTCTGTACGTCGATCTACTACCTACTCATCTCTTCATGCATCAGAAAATAGGGA
  ACTATTAAATGTACACAGTGCCAACGTTTCAAAAGTTTCTTTTAATGAGAAAGCTGTAACTGAAACTTCC
  TTTAATTCCGTAAATGTTAATGGCCAAAGAGGAGAGAATAGTAAACTTAGTCTTACCCCCAACTGTTCTT
  CAACTTTGAACATTACACAAAGCCAAATACATTTTCTAAGTCCAGATTCTTTTGTAAATAATAGTCATGG
  AGCTAATAATGAACTAGAATTAGTAACATGTCTTTCATCAGATATGTTTATGAAAGATAATTCACAGCCT
  GTGCATTTGGAATCAACAATTGCACATGAAATTTATCAGAAAATTTTAAGTCCAGATTCTTTCATAAAAG
  ATAATTATGGACTAAATCAGGATCTAGAATCAGAGTCAGTTAATCCTATTTTATCCCCTAATCAATTTTT
  AAAAGATAACATGGCATATATGTGTACATCTCAGCAAACATGTAAAGTACCATTATCAAATGAAAATTCT
  CAAGTCCCACAGTCTCCTGAAGATTGGAGAAAAAGTGAAGTTTCGCCACGTATTCCTGAATGTCAGGGTT
  CAAAATCTCCCAAAGCTATTTTTGAAGAACTAGTAGAAATGAAGTCAAATTACTACAGTTTTATAAAACA
  AAATAATCCTAAATTTTCTGCAGTTCAGGATATTTCTAGTCATAGCCACAATAAACAACCTAAGAGACGT
  CCAATACTTTCTGCCACTGTTACTAAAAGGAAGGCCACCTGTACCAGAGAAAACCAAACTGAGATTAATA
  AACCAAAAGCAAAAAGATGTCTCAACAGTGCAGTGGGTGAACATGAAAAAGTAATAAATAATCAAAAGGA
  AAAAGAAGATTTTCATTCTTATCTTCCAATTATAGATCCAATATTAAGTAAATCTAAGAGTTATAAAAAC
  GAGGTAACACCCTCTTCGACAACAGCTTCAGTTGCTCGGAAAAGAAAGAGCGATGGAAGCATGGAAGATG
  CAAATGTGAGAGTTGCAATTACAGAACATACAGAAGTGCGAGAAATCAAAAGAATCCATTTTTCTCCCTC
  AGAGCCTAAAACATCAGCTGTTAAGAAAACAAAAAATGTGACAACACCCATCTCAAAACGTATTAGCAAC
  AGAGAGAAATTAAACCTGAAGAAGAAAACTGATTTATCAATATTCAGAACTCCAATTTCTAAAACAAACA
  AAAGGACAAAACCCATTATCGCTGTGGCACAGTCCAGTTTGACCTTCATAAAACCATTAAAAACAGATAT
  TCCCAGACACCCGATGCCATTTGCTGCAAAAAACATGTTTTATGATGAACGCTGGAAGGAAAAGCAGGAA
  CAGGGCTTCACTTGGTGGTTAAATTTTATATTAACCCCTGATGACTTCACTGTAAAAACAAATATTTCTG
  AAGTAAATGCTGCTACTCTTCTTTTGGGAATAGAGAATCAACATAAAATAAGTGTTCCTAGAGCACCTAC
  AAAAGAGGAAATGTCTCTCAGAGCTTATACTGCTCGGTGTAGGTTAAACAGACTACGTCGTGCAGCATGC
  CGTTTGTTTACTTCTGAAAAAATGGTTAAAGCTATTAAAAAGCTTGAAATTGAAATTGAAGCTAGGCGGT
  TAATTGTTCGAAAAGATAGACACCTATGGAAAGATGTGGGAGAACGTCAGAAAGTCCTGAATTGGCTGTT
  GTCCTACAATCCTTTGTGGCTTCGAATTGGTCTAGAGACAACTTATGGAGAACTCATATCTTTGGAAGAT
  AACAGTGATGTCACAGGGTTGGCTATGTTTATTCTGAATCGCCTACTTTGGAATCCTGATATAGCAGCTG
  AGTATAGACACCCCACTGTTCCTCACCTGTATAGAGATGGTCATGAAGAAGCTTTGTCCAAGTTTACATT
  GAAAAAGTTATTGTTGTTGGTCTGTTTTCTTGATTATGCTAAAATTTCCAGACTCATTGATCATGATCCT
  TGTCTCTTCTGTAAAGATGCCGAATTCAAGGCTAGTAAAGAAATCCTTTTGGCTTTTTCACGAGATTTCC
  TAAGTGGTGAAGGTGACCTTTCCCGTCACCTTGGCTTATTGGGATTACCTGTTAACCATGTTCAGACACC
  ATTTGATGAATTTGATTTTGCCGTTACAAATCTTGCCGTAGACTTGCAATGTGGAGTGCGCCTTGTGCGA
  ACCATGGAACTTCTCACACAGAACTGGGACCTCTCAAAGAAACTCAGGATTCCGGCAATAAGTCGTCTTC
  AAAAGATGCACAATGTTGACATTGTTCTTCAAGTTCTTAAATCACGAGGAATTGAATTAAGTGATGAGCA
  TGGAAATACAATTCTATCTAAGGATATTGTGGATAGGCACAGAGAAAAAACTCTCAGGTTGCTTTGGAAA
  ATAGCGTTTGCTTTTCAGGTGGATATTTCCCTTAACTTAGATCAATTAAAGGAAGAAATTGCCTTTCTAA
  AACACACAAAGAGTATAAAGAAAACAATATCTCTACTATCATGCCATTCTGATGATCTTATTAATAAGAA
  AAAAGGCAAAAGGGATAGTGGTTCCTTTGAACAATATAGTGAAAACATAAAGTTATTGATGGATTGGGTA
  AATGCTGTTTGTGCCTTCTATAATAAAAAGGTGGAGAATTTTACAGTGTCTTTCTCAGACGGCCGTGTGT
  TATGTTACCTGATCCACCATTACCATCCTTGCTATGTGCCATTTGACGCTATATGTCAGCGTACTACTCA
  AACTGTGGAATGTACGCAAACTGGTTCAGTGGTATTAAATTCATCATCTGAATCTGATGACAGTTCTCTG
  GATATGTCTCTTAAAGCATTTGATCATGAAAATACTTCAGAGCTATACAAAGAGCTCCTAGAAAATGAAA
  AGAAAAATTTTCACTTGGTTAGGTCTGCAGTTAGAGACCTTGGTGGAATACCTGCTATGATTAATCATTC
  AGATATGTCAAATACAATTCCAGATGAAAAGGTGGTTATTACCTATTTGTCATTTCTTTGTGCAAGGCTT
  TTGGATCTTCGTAAAGAAATAAGAGCTGCTCGACTCATACAAACAACATGGAGAAAATATAAACTAAAAA
  CAGATCTCAAACGCCATCAGGAGAGAGAGAAAGCTGCAAGAATTATTCAATTGGCTGTAATCAATTTTCT
  AGCAAAACAAAGATTGAGAAAAAGAGTTAATGCAGCACTCGTCATTCAGAAATATTGGCGAAGAGTCTTA
  GCACAGAGAAAATTATTAATGTTAAAAAAGGAAAAGCTGGAAAAAGTTCAAAATAAAGCAGCATCACTTA
  TTCAGGGATATTGGAGAAGATATTCCACTAGACAAAGATTTCTGAAATTGAAATATTATTCAATCATCCT
  GCAATCTAGGATAAGAATGATAATTGCTGTTACATCTTATAAACGATATCTTTGGGCTACAGTTACAATT
  CAGAGGCATTGGCGTGCTTATTTAAGAAGAAAACAAGATCAACAAAGATATGAAATGCTAAAATCATCAA
  CTCTTATAATCCAATCTATGTTCAGAAAATGGAAGCAACGTAAAATGCAATCACAAGTAAAAGCTACAGT
  AATATTGCAAAGAGCTTTTAGAGAATGGCATTTAAGAAAACAAGCTAAAGAAGAAAATTCTGCTATTATC
  ATACAATCATGGTATAGAATGCATAAAGAATTACGGAAATATATTTATATTAGATCTTGTGTTGTTATCA
  TTCAGAAAAGATTTCGGTGCTTTCAAGCCCAAAAGTTATATAAAAGAAGAAAAGAGTCCATACTAACCAT
  CCAGAAGTACTACAAAGCATATCTGAAAGGAAAGATTGAGCGCACCAACTATTTGCAGAAACGAGCTGCA
  GCCATTCAATTACAAGCTGCTTTTAGGAGACTGAAAGCTCATAATTTATGTAGACAAATTAGAGCTGCTT
  GTGTTATTCAGTCATACTGGAGAATGAGACAAGACAGAGTTCGATTTTTAAACCTTAAGAAGACTATTAT
  CAAATTTCAGGCACATGTAAGAAAACATCAACAACGACAGAAATATAAGAAGATGAAGAAAGCAGCTGTT
  ATAATTCAGACTCATTTCCGAGCTTATATTTTTGCCATGAAAGTTCTAGCATCTTACCAGAAAACACGCT
  CTGCTGTCATTGTGCTGCAGTCTGCATATAGAGGGATGCAAGCCAGGAAAATGTATATTCACATCCTCAC
  ATCTGTTATAAAGATTCAATCATATTATCGTGCTTATGTTTCTAAAAAGGAATTTTTGAGCCTAAAAAAT
  GCTACAATAAAATTGCAGTCAACTGTTAAGATGAAACAAACACGTAAACAATATTTGCATTTAAGAGCAG
  CTGCACTATTTATCCAGCAATGTTACCGTTCCAAAAAAATAGCTGCACAAAAGAGAGAAGAGTATATGCA
  GATGCGGGAATCTTGTATCAAACTGCAAGCATTTGTTAGAGGATACCTTGTCCGAAAGCAGATGAGGTTA
  CAAAGAAAAGCTGTTATTTCACTACAGTCTTATTTCAGAATGAGAAAGGCTCGGCAGTATTATCTGAAAA
  TGTATAAAGCAATTATTGTCATTCAGAATTACTATCATGCATACAAAGCACAGGTCAATCAGAGGAAGAA
  CTTCTTGCAAGTCAAAAAAGCAGCTACTTGCTTGCAAGCAGCTTACAGAGGTTATAAAGTACGCCAGCTA
  ATCAAACAACAATCTATAGCTGCTCTTAAAATTCAGTCTGCTTTTAGAGGCTATAATAAAAGGGTAAAAT
  ATCAATCTGTGCTTCAATCTATAATAAAGATTCAGAGATGGTACAGGGCGTACAAGACTCTTCATGATAC
  AAGAACACATTTTTTGAAGACAAAGGCAGCTGTGATTTCCCTCCAGTCTGCTTATCGTGGCTGGAAGGTT
  CGGAAACAGATTAGAAGGGAACATCAAGCTGCCTTGAAGATTCAGTCTGCTTTTAGAATGGCCAAGGCCC
  AGAAACAGTTTAGATTGTTTAAAACAGCAGCATTAGTCATCCAGCAAAATTTCAGAGCATGGACTGCAGG
  AAGGAAGCAATGTATGGAGTATATTGAACTCCGTCATGCGGTACTGGTGCTTCAATCTATGTGGAAGGGA
  AAAACACTGAGAAGACAGCTTCAAAGGCAACATAAATGTGCTATCATCATACAGTCATACTATAGAATGC
  ATGTGCAACAAAAGAAGTGGAAAATCATGAAAAAAGCTGCTCTTCTGATTCAAAAGTATTATAGGGCTTA
  CAGTATTGGAAGAGAACAGAATCATTTATATTTGAAAACAAAAGCAGCTGTAGTAACTTTACAGTCAGCT
  TATCGTGGTATGAAAGTGAGAAAAAGAATAAAGGATTGCAACAAAGCAGCAGTCACTATACAGTCTAAAT
  ACAGAGCTTACAAAACCAAAAAGAAATATGCAACCTATAGAGCTTCAGCTATTATAATTCAGAGATGGTA
  TCGAGGTATTAAAATTACAAACCATCAGCATAAGGAGTATCTTAATTTGAAGAAGACAGCAATTAAAATC
  CAATCTGTTTATAGAGGTATTAGAGTTAGAAGACATATTCAACACATGCACAGGGCAGCCACTTTTATTA
  AAGCCATGTTTAAAATGCATCAGTCAAGAATAAGTTACCATACAATGAGAAAAGCAGCTATTGTTATTCA
  AGTAAGATGTAGAGCATATTATCAAGGTAAAATGCAGCGTGAAAAGTACCTGACAATTTTGAAAGCTGTT
  AAAGTCCTTCAGGCAAGTTTTAGAGGAGTAAGAGTTAGACGGACTCTTAGAAAGATGCAGACTGCAGCAA
  CACTCATTCAGTCAAACTACAGAAGATACAGACAGCAAACATACTTTAATAAGTTAAAGAAAATAACAAA
  AACAGTACAGCAAAGATACTGGGCAATGAAAGAAAGAAACATACAATTTCAAAGGTATAACAAACTGAGG
  CATTCTGTAATATACATTCAGGCTATTTTTAGGGGAAAGAAAGCTAGAAGACATTTAAAAATGATGCATA
  TAGCCGCAACTCTCATTCAGAGGAGATTTAGAACTCTAATGATGAGAAGAAGATTCCTCTCTCTCAAGAA
  AACTGCTATTTTGATTCAGAGAAAATATCGGGCACATCTTTGTACAAAGCATCACTTACAGTTCCTTCAG
  GTACAAAATGCAGTTATTAAAATCCAGTCATCATACAGAAGATGGATGATAAGGAAAAGGATGCGAGAGA
  TGCACAGGGCTGCTACTTTCATCCAGTCTACTTTCAGAATGCACAGATTACATATGAGATATCAGGCTTT
  GAAACAGGCCTCCGTTGTGATCCAACAGCAATACCAAGCAAATAGAGCTGCAAAACTGCAGAGGCAGCAT
  TATCTCAGACAAAGACACTCTGCTGTGATCCTTCAGGCTGCATTCAGGGGTATGAAAACTAGAAGACATT
  TGAAGAGTATGCATTCCTCTGCAACCCTTATTCAGAGTAGGTTTAGATCATTACTGGTGAGGAGAAGATT
  CATTTCCCTCAAAAAAGCTACTATTTTTGTTCAGAGGAAATATCGAGCCACCATTTGTGCCAAACATAAA
  TTGTACCAATTCTTGCACTTAAGAAAGGCAGCCATTACAATACAGTCATCTTACAGAAGACTGATGGTAA
  AGAAGAAGTTACAAGAAATGCAAAGGGCTGCAGTTCTCATTCAGGCTACTTTCAGGATGTACAGAACATA
  TATTACATTTCAGACTTGGAAACATGCTTCAATTCTAATTCAGCAACATTATCGAACATATAGAGCTGCA
  AAATTACAAAGAGAAAATTATATCAGACAATGGCATTCTGCTGTGGTTATTCAGGCTGCATATAAAGGAA
  TGAAAGCAAGACAACTTTTAAGGGAAAAACACAAAGCTTCTATCGTAATACAAAGCACCTACAGAATGTA
  TAGGCAGTATTGTTTCTACCAAAAGCTTCAGTGGGCTACAAAAATCATACAAGAAAAATATAGAGCAAAT
  AAAAAGAAACAGAAAGTATTTCAACACAATGAACTTAAGAAAGAGACTTGTGTTCAGGCAGGTTTTCAGG
  ACATGAACATAAAAAAACAGATTCAGGAACAGCACCAGGCTGCCATTATTATTCAGAAGCATTGTAAAGC
  CTTTAAAATAAGGAAGCATTATCTCCACCTTAGAGCAACAGTAGTTTCTATTCAAAGAAGATACAGAAAA
  CTAACTGCAGTGCGTACCCAAGCAGTTATTTGTATACAGTCTTATTACAGAGGCTTTAAAGTACGAAAGG
  ATATTCAAAATATGCACCGGGCTGCCACACTAATTCAGTCATTCTATCGAATGCACAGGGCCAAAGTTGA
  TTATGAAACAAAGAAAACTGCAATTGTGGTTATACAGAATTATTATAGGTTGTATGTTAGAGTAAAAACA
  GAAAGAAAAAACTTTTTAGCAGTTCAGAAATCTGTACGAACTATTCAGGCTGCTTTTAGAGGCATGAAAG
  TTAGACAAAAATTGAAAAATGTATCAGAGGAAAAGATGGCAGCCATTGTTAACCAATCTGCACTCTGCTG
  TTACAGAAGTAAAACTCAGTATGAAGCTGTTCAAAGTGAAGGTGTTATGATTCAAGAGTGGTATAAAGCT
  TCTGGCCTTGCTTGTTCACAGGAAGCAGAGTATCATTCTCAAAGTAGGGCTGCAGTAACAATTCAAAAAG
  CTTTTTGTAGAATGGTCACAAGAAAACTGGAAACACAGAAATGTGCTGCCCTACGGATTCAGTTCTTCCT
  TCAGATGGCTGTGTATCGGAGAAGATTTGTTCAGCAGAAAAGAGCTGCTATCACTTTACAGCATTATTTT
  AGGACGTGGCAAACCAGAAAACAGTTTTTACTATATAGAAAAGCAGCAGTGGTTTTACAAAATCACTACA
  GAGCATTTCTGTCTGCAAAACATCAAAGACAAGTCTATTTACAGATCAGAAGCAGTGTTATCATTATTCA
  AGCTAGAAGTAAAGGATTTATACAGAAACGGAAGTTTCAGGAAATTAAAAATAGCACCATAAAAATTCAG
  GCTATGTGGAGGAGATATAGAGCCAAGAAATATTTATGTAAAGTGAAAGCTGCCTGCAAGATTCAAGCCT
  GGTATAGATGTTGGAGAGCACACAAAGAATATCTAGCTATATTAAAAGCTGTTAAAATTATTCAAGGTTG
  CTTCTATACCAAACTAGAGAGAACACGGTTTTTGAATGTGAGAGCATCAGCAATTATCATTCAGAGAAAA
  TGGAGAGCTATACTTCCTGCAAAGATAGCTCATGAACACTTCTTAATGATAAAAAGACATCGAGCTGCTT
  GTTTGATCCAAGCACATTATAGAGGATATAAAGGAAGGCAGGTCTTTCTTCGGCAGAAATCTGCTGCTTT
  GATCATACAAAAATATATACGAGCCAGGGAGGCTGGAAAGCATGAAAGGATAAAATATATTGAATTTAAA
  AAATCTACAGTTATCCTACAAGCACTGGTGCGTGGTTGGCTAGTACGAAAAAGATTTTTAGAACAGAGAG
  CCAAAATTCGACTTCTTCACTTCACTGCAGCTGCATATTATCACCTGAATGCTGTTAGAATTCAAAGAGC
  CTATAAACTTTACCTGGCTGTGAAGAATGCTAACAAGCAGGTTAATTCAGTCATCTGTATTCAGAGATGG
  TTTCGAGCAAGATTACAAGAAAAGAGATTTATTCAGAAATATCATAGCATCAAAAAGATTGAGCATGAAG
  GTCAAGAATGTCTGAGCCAGCGAAATAGGGCTGCATCAGTAATACAGAAAGCAGTGCGCCATTTTCTCCT
  CCGTAAAAAGCAGGAAAAATTCACTAGTGGAATCATTAAAATTCAGGCATTATGGAGAGGCTATTCTTGG
  AGGAAGAAAAATGATTGTACAAAAATTAAAGCTATACGACTAAGTCTTCAAGTTGTTAATAGGGAGATTC
  GAGAAGAAAACAAACTCTACAAAAGAACTGCACTTGCACTTCATTACCTTTTGACATATAAGCACCTTTC
  TGCCATTCTTGAGGCCTTAAAACACCTAGAGGTAGTTACTAGATTGTCTCCACTTTGTTGTGAGAACATG
  GCCCAGAGTGGAGCAATTTCTAAAATATTTGTTTTGATCCGAAGTTGTAATCGCAGTATTCCTTGTATGG
  AAGTCATCAGATATGCTGTGCAAGTCTTGCTTAATGTATCTAAGTATGAGAAAACTACTTCAGCAGTTTA
  TGATGTAGAAAATTGTATAGATATACTATTGGAGCTTTTGCAGATATACCGAGAAAAGCCTGGTAATAAA
  GTTGCAGACAAAGGCGGAAGCATTTTTACAAAAACTTGTTGTTTGTTGGCTATTTTACTGAAGACAACAA
  ATAGAGCCTCTGATGTACGAAGTAGGTCCAAAGTTGTTGACCGTATTTACAGTCTCTACAAACTTACAGC
  TCATAAACATAAAATGAATACTGAAAGAATACTTTACAAGCAAAAGAAGAATTCTTCTATAAGCATTCCT
  TTTATCCCAGAAACACCTGTAAGGACCAGAATAGTTTCAAGACTTAAGCCAGATTGGGTTTTGAGAAGAG
  ATAACATGGAAGAAATCACAAATCCCCTGCAAGCTATTCAAATGGTGATGGATACGCTTGGCATTCCTTA
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214770 representing NM_018136
  Red=Cloning site Green=Tags(s)
  
  MANRRVGRGCWEVSPTERRPPAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGASRTLSLALDNP
  NEEVAEVKISHFPAADLGFSVSQRCFVLQPKEKIVISVNWTPLKEGRVREIMTFLVNDVLKHQAILLGNA
  EEQKKKKRSLWDTIKKKKISASTSHNRRVSNIQNVNKTFSVSQKVDRVRSPLQACENLAMNEGGPPTENN
  SLILEENKIPISPISPAFNECHGATCLPLSVRRSTTYSSLHASENRELLNVHSANVSKVSFNEKAVTETS
  FNSVNVNGQRGENSKLSLTPNCSSTLNITQSQIHFLSPDSFVNNSHGANNELELVTCLSSDMFMKDNSQP
  VHLESTIAHEIYQKILSPDSFIKDNYGLNQDLESESVNPILSPNQFLKDNMAYMCTSQQTCKVPLSNENS
  QVPQSPEDWRKSEVSPRIPECQGSKSPKAIFEELVEMKSNYYSFIKQNNPKFSAVQDISSHSHNKQPKRR
  PILSATVTKRKATCTRENQTEINKPKAKRCLNSAVGEHEKVINNQKEKEDFHSYLPIIDPILSKSKSYKN
  EVTPSSTTASVARKRKSDGSMEDANVRVAITEHTEVREIKRIHFSPSEPKTSAVKKTKNVTTPISKRISN
  REKLNLKKKTDLSIFRTPISKTNKRTKPIIAVAQSSLTFIKPLKTDIPRHPMPFAAKNMFYDERWKEKQE
  QGFTWWLNFILTPDDFTVKTNISEVNAATLLLGIENQHKISVPRAPTKEEMSLRAYTARCRLNRLRRAAC
  RLFTSEKMVKAIKKLEIEIEARRLIVRKDRHLWKDVGERQKVLNWLLSYNPLWLRIGLETTYGELISLED
  NSDVTGLAMFILNRLLWNPDIAAEYRHPTVPHLYRDGHEEALSKFTLKKLLLLVCFLDYAKISRLIDHDP
  CLFCKDAEFKASKEILLAFSRDFLSGEGDLSRHLGLLGLPVNHVQTPFDEFDFAVTNLAVDLQCGVRLVR
  TMELLTQNWDLSKKLRIPAISRLQKMHNVDIVLQVLKSRGIELSDEHGNTILSKDIVDRHREKTLRLLWK
  IAFAFQVDISLNLDQLKEEIAFLKHTKSIKKTISLLSCHSDDLINKKKGKRDSGSFEQYSENIKLLMDWV
  NAVCAFYNKKVENFTVSFSDGRVLCYLIHHYHPCYVPFDAICQRTTQTVECTQTGSVVLNSSSESDDSSL
  DMSLKAFDHENTSELYKELLENEKKNFHLVRSAVRDLGGIPAMINHSDMSNTIPDEKVVITYLSFLCARL
  LDLRKEIRAARLIQTTWRKYKLKTDLKRHQEREKAARIIQLAVINFLAKQRLRKRVNAALVIQKYWRRVL
  AQRKLLMLKKEKLEKVQNKAASLIQGYWRRYSTRQRFLKLKYYSIILQSRIRMIIAVTSYKRYLWATVTI
  QRHWRAYLRRKQDQQRYEMLKSSTLIIQSMFRKWKQRKMQSQVKATVILQRAFREWHLRKQAKEENSAII
  IQSWYRMHKELRKYIYIRSCVVIIQKRFRCFQAQKLYKRRKESILTIQKYYKAYLKGKIERTNYLQKRAA
  AIQLQAAFRRLKAHNLCRQIRAACVIQSYWRMRQDRVRFLNLKKTIIKFQAHVRKHQQRQKYKKMKKAAV
  IIQTHFRAYIFAMKVLASYQKTRSAVIVLQSAYRGMQARKMYIHILTSVIKIQSYYRAYVSKKEFLSLKN
  ATIKLQSTVKMKQTRKQYLHLRAAALFIQQCYRSKKIAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRL
  QRKAVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQL
  IKQQSIAALKIQSAFRGYNKRVKYQSVLQSIIKIQRWYRAYKTLHDTRTHFLKTKAAVISLQSAYRGWKV
  RKQIRREHQAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQCMEYIELRHAVLVLQSMWKG
  KTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAYSIGREQNHLYLKTKAAVVTLQSA
  YRGMKVRKRIKDCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKI
  QSVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRCRAYYQGKMQREKYLTILKAV
  KVLQASFRGVRVRRTLRKMQTAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYWAMKERNIQFQRYNKLR
  HSVIYIQAIFRGKKARRHLKMMHIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQ
  VQNAVIKIQSSYRRWMIRKRMREMHRAATFIQSTFRMHRLHMRYQALKQASVVIQQQYQANRAAKLQRQH
  YLRQRHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHK
  LYQFLHLRKAAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMYRTYITFQTWKHASILIQQHYRTYRAA
  KLQRENYIRQWHSAVVIQAAYKGMKARQLLREKHKASIVIQSTYRMYRQYCFYQKLQWATKIIQEKYRAN
  KKKQKVFQHNELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRK
  LTAVRTQAVICIQSYYRGFKVRKDIQNMHRAATLIQSFYRMHRAKVDYETKKTAIVVIQNYYRLYVRVKT
  ERKNFLAVQKSVRTIQAAFRGMKVRQKLKNVSEEKMAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKA
  SGLACSQEAEYHSQSRAAVTIQKAFCRMVTRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYF
  RTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVIIIQARSKGFIQKRKFQEIKNSTIKIQ
  AMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRASAIIIQRK
  WRAILPAKIAHEHFLMIKRHRAACLIQAHYRGYKGRQVFLRQKSAALIIQKYIRAREAGKHERIKYIEFK
  KSTVILQALVRGWLVRKRFLEQRAKIRLLHFTAAAYYHLNAVRIQRAYKLYLAVKNANKQVNSVICIQRW
  FRARLQEKRFIQKYHSIKKIEHEGQECLSQRNRAASVIQKAVRHFLLRKKQEKFTSGIIKIQALWRGYSW
  RKKNDCTKIKAIRLSLQVVNREIREENKLYKRTALALHYLLTYKHLSAILEALKHLEVVTRLSPLCCENM
  AQSGAISKIFVLIRSCNRSIPCMEVIRYAVQVLLNVSKYEKTTSAVYDVENCIDILLELLQIYREKPGNK
  VADKGGSIFTKTCCLLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILYKQKKNSSISIP
  FIPETPVRTRIVSRLKPDWVLRRDNMEEITNPLQAIQMVMDTLGIPY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018136
• ORF Size: 10431 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018136.5
• RefSeq Size: 10906 bp
• RefSeq ORF: 10434 bp
• Locus ID: 259266
• UniProt ID: Q8IZT6
• Cytogenetics: 1q31.3
• Domains: IQ, CH
• Protein Families: Druggable Genome
• Gene Summary: This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]