RAB11FIP1 (NM_001002814) Human Tagged ORF Clone

CAT#: RG214680

RAB11FIP1 (tGFP-tagged) - Human RAB11 family interacting protein 1 (class I) (RAB11FIP1), transcript variant 3

Product Images

Circular map for RG214680

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RAB11FIP1
• Synonyms: NOEL1A; rab11-FIP1; RCP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214680 representing NM_001002814
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTAATGGTCTCGGCTGGCCGGGGCCTGGGGGCCGTGTGGTCCCCAACCCACGTGCAGGTGACGG
  TGCTGCAGGCGCGGGGCCTGCGGGCCAAGGGCCCCGGGGGCACGAGCGACGCGTACGCGGTGATCCAGGT
  GGGCAAGGAGAAGTACGCCACCTCCGTGTCGGAGCGCAGCCTGGGCGCGCCCGTGTGGCGCGAGGAGGCC
  ACCTTCGAGCTGCCATCGCTGCTGTCCTCCGGACCCGCGGCCGCCGCCACCCTGCAGCTCACCGTGCTGC
  ACCGCGCGCTGCTCGGCCTCGACAAGTTCCTGGGCCGCGCCGAGGTGGACCTGCGGGATCTGCACCGCGA
  CCAGGGCCGCAGGAAGACGCAGTGGTATAAGTTGAAATCCAAACCAGGAAAGAAGGACAAGGAGCGAGGA
  GAAATTGAGGTTGACATCCAGTTTATGAGAAACAACATGACTGCCAGCATGTTTGACCTTTCTATGAAAG
  ACAAGTCTCGGAATCCATTTGGAAAGCTGAAGGACAAGATCAAGGGGAAGAATAAGGACAGTGGGTCAGA
  CACCGCCTCCGCCATCATCCCTAGCACGACACCTTCGGTCGACAGTGATGATGAGTCTGTGGTTAAAGAC
  AAGAAAAAGAAATCAAAGATCAAGACCTTACTTTCCAAGTCAAATTTGCAGAAGACGCCTCTTTCCCAGT
  CCATGTCTGTCCTGCCGACTTCAAAGCCAGAAAAAGTGCTGCTTCGTCCCGGAGACTTTCAGTCCCAGTG
  GGATGAAGATGACAATGAGGATGAGTCCTCCTCGGCCTCGGATGTCATGTCTCACAAGAGAACAGCGAGT
  ACGGATCTTAAGCAACTGAACCAGGTCAACTTTACCCTTCCCAAGAAGGAAGGACTTTCCTTTCTTGGTG
  GCCTTCGGTCTAAGAATGATGTCCTTTCCCGCTCTAATGTCTGCATCAATGGGAACCATGTTTACCTGGA
  GCAGCCAGAAGCCAAGGGTGAGATCAAGGATAGCAGCCCGTCCTCCTCCCCATCCCCCAAGGGCTTCAGA
  AAGAAGCATTTGTTCTCTTCTACAGAGAACCTGGCGGCTGGGTCTTGGAAGGAGCCTGCTGAAGGAGGTG
  GGCTGTCTTCTGACAGGCAGCTCTCCGAATCTTCCACCAAGGACTCCTTGAAGTCTATGACCCTGCCGTC
  CTACCGACCTGCCCCACTGGTCAGTGGGGACCTCAGGGAAAACATGGCCCCCGCAAACTCAGAGGCCACA
  AAAGAAGCTAAGGAGAGCAAGAAGCCAGAGAGCAGGAGGTCCTCTTTGCTGTCTCTGATGACGGGGAAGA
  AGGATGTGGCTAAGGGCAGTGAAGGTGAAAACCCTCTCACGGTCCCAGGGAGGGAGAAGGAAGGCATGCT
  GATGGGGGTTAAGCCGGGGGAGGACGCATCGGGGCCTGCTGAAGACCTTGTGAGAAGATCTGAGAAAGAT
  ACTGCAGCTGTTGTCTCCAGACAGGGCAGCTCCCTGAACCTCTTTGAAGATGTGCAGATCACAGAACCAG
  AAGCTGAGCCAGAGTCCAAGTCTGAACCGAGACCTCCAATTTCCTCTCCGAGGGCTCCCCAGACCAGAGC
  TGTCAAGCCCCGACTGGAAGTGTCTCCAGAGGCGCAACCCACAGCCAGGCTTCCTTCCCCTACTGACTCC
  CCTTCCTCTCTTCCTCCTCTTCCTTCTAGCTCTGGCCAGGCATCTGCCCCCTCTGAATTGGGACATGGTG
  CAGACACACAGTCCTCTGAGAGTCCTTCTGTCTTCTCCTCTCTCTCATCTCCCATAGCAGCTCCCATTTC
  CACATCCACTCCAATTGAAAGCTGGCCTCTCGTAGACAGGGGCCAGGCCAAGTCTGAAGGACCACCCTTG
  CTCCCTAAGGCAGAGTTGCAAACTGAGAGTTTAACACCGGTTCCAAATTCTGGTTCTTCTGCCCTGGGAT
  CACTTTTCAAACAGCCATCCTTTCCAGCAAATAAAGGGACAGAAGATTCTCTGATGGGCAGGACCCGTGA
  GACAGGCACAGAGAAGAACACCAGCAGCCTTGAGTTGGAGGAGTCTCTCCCAGAGCAGCCTGAAACAGGG
  CGACAAGAGGAAGAACTTCCGAGATTCCCCTGCAAAAAACAAGACTACAGCCCATCATCTGGAGAAGCCC
  AGGAAGTACCGTTTGCCCTTTCACTCAGCAGTGATGGAGCTGTGAGCCCTGTTGGGGAGCTTGCAGCAGG
  AGGAGACAGAGACTTGGAGAGTCAGGCTGGGTCTCTTGTGGAGAGCAAAGCCAGGGATGCAGCTGAAGAA
  GTGGCGCCCCCTCTTCCCATGGGAGCATCAGTCCCTTCCATTGATTCCATGATGCGGAAGCTGGAAGAGA
  TGGGTCTGAACCTCCGCAAGGACCAGAAGAAAACCAAGAAGCGTGTGTCATTTTCTGAGCAGCTCTTCAC
  GGAAGAGGCAGTGGCGGGGGCTGCCTTGCTGGTGGAAGGACACAGCAGTTGTCCCCAGGAGCTGAACCCT
  GCATGGTCTGTTGCTGGAAACGCGTCTGACGGAGAGCCTCCTGAGTCTCCCCACGCAGAGGACTCAGAAA
  GGGAATCGGTGACCACACCTGGGCCAGCGACGTGTGGTGCGCCAGCCTCCCCAGCGGATCACCTCCTCCT
  CCCCTCCCAGGAGGAGAGTTTCTCCGAAGTCCCCATGAGTGAAGCAAGCTCAGCGAAAGACACTCCACTC
  TTTAGGATGGAGGGAGAGGATGCCCTTGTGACTCAGTATCAGAGCAAAGCCAGTGACCACGAAGGTTTAT
  TGTCTGACCCCTTGAGTGACTTTCAGTTGGTCTCAGATTTTAAATCTCCAATCATGGCCGATCTGAACTT
  AAGCCTTCCTTCCATTCCTGAAGTCGCATCGGATGATGAAAGAATAGATCAGGTTGAAGATGACGGAGAT
  CAGGTTGAAGATGATGGAGAGACAGCAAAGTCGTCAACTCTGGACATAGGAGCTTTGTCCTTGGGCTTGG
  TAGTCCCCTGTCCTGAGAGGGGAAAGGGGCCCAGTGGCGAGGCAGATAGGTTGGTACTGGGGGAGGGCCT
  GTGTGATTTCAGGCTGCAAGCACCCCAGGCATCTGTGACAGCTCCTTCAGAGCAGACCACAGAGTTCGGA
  ATTCACAAACCACATCTTGGCAAGAGCTCAAGCTTGGATAAACAGCTGCCAGGCCCCAGTGGTGGTGAGG
  AAGAAAAACCGATGGGAAATGGGAGTCCAAGCCCGCCTCCTGGCACATCCCTGGACAATCCTGTACCCAG
  CCCCTCCCCTTCTGAGATCTTTCCTGTCACACACTCTTTCCCCAGCTCTGCACATTCTGACACTCACCAC
  ACCAGCACAGCAGAATCTCAAAAAAAAGCCACAGCAGAGGGCTCCGCTGGTAGAGTTGAAAATTTTGGCA
  AGAGGAAGCCACTCCTCCAGGCCTGGGTCTCACCCTCGGAGACACATCCAGTCTCAGCTCAGCCAGGCGC
  TGGAACTGGGTCAGCCAAGCACAGACTTCATCCTGTGAAGCCAATGAATGCCACGGCCACCAAGGTTGCT
  AACTGCAGCTTGGGAACTGCCACCATCATCAGTGAGAACTTGAACAATGAGGTCATGATGAAGAAATACA
  GCCCCTCGGACCCTGCATTTGCATATGCGCAGCTGACCCACGATGAGCTGATTCAGCTGGTCCTCAAACA
  GAAGGAAACGATAAGCAAGAAGGAGTTCCAGGTCCGCGAGCTGGAAGACTACATTGACAACCTGCTTGTC
  AGGGTCATGGAAGAAACCCCCAATATCCTCCGCATCCCGACTCAGGTTGGCAAAAAAGCAGGAAAGATG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214680 representing NM_001002814
  Red=Cloning site Green=Tags(s)
  
  MSLMVSAGRGLGAVWSPTHVQVTVLQARGLRAKGPGGTSDAYAVIQVGKEKYATSVSERSLGAPVWREEA
  TFELPSLLSSGPAAAATLQLTVLHRALLGLDKFLGRAEVDLRDLHRDQGRRKTQWYKLKSKPGKKDKERG
  EIEVDIQFMRNNMTASMFDLSMKDKSRNPFGKLKDKIKGKNKDSGSDTASAIIPSTTPSVDSDDESVVKD
  KKKKSKIKTLLSKSNLQKTPLSQSMSVLPTSKPEKVLLRPGDFQSQWDEDDNEDESSSASDVMSHKRTAS
  TDLKQLNQVNFTLPKKEGLSFLGGLRSKNDVLSRSNVCINGNHVYLEQPEAKGEIKDSSPSSSPSPKGFR
  KKHLFSSTENLAAGSWKEPAEGGGLSSDRQLSESSTKDSLKSMTLPSYRPAPLVSGDLRENMAPANSEAT
  KEAKESKKPESRRSSLLSLMTGKKDVAKGSEGENPLTVPGREKEGMLMGVKPGEDASGPAEDLVRRSEKD
  TAAVVSRQGSSLNLFEDVQITEPEAEPESKSEPRPPISSPRAPQTRAVKPRLEVSPEAQPTARLPSPTDS
  PSSLPPLPSSSGQASAPSELGHGADTQSSESPSVFSSLSSPIAAPISTSTPIESWPLVDRGQAKSEGPPL
  LPKAELQTESLTPVPNSGSSALGSLFKQPSFPANKGTEDSLMGRTRETGTEKNTSSLELEESLPEQPETG
  RQEEELPRFPCKKQDYSPSSGEAQEVPFALSLSSDGAVSPVGELAAGGDRDLESQAGSLVESKARDAAEE
  VAPPLPMGASVPSIDSMMRKLEEMGLNLRKDQKKTKKRVSFSEQLFTEEAVAGAALLVEGHSSCPQELNP
  AWSVAGNASDGEPPESPHAEDSERESVTTPGPATCGAPASPADHLLLPSQEESFSEVPMSEASSAKDTPL
  FRMEGEDALVTQYQSKASDHEGLLSDPLSDFQLVSDFKSPIMADLNLSLPSIPEVASDDERIDQVEDDGD
  QVEDDGETAKSSTLDIGALSLGLVVPCPERGKGPSGEADRLVLGEGLCDFRLQAPQASVTAPSEQTTEFG
  IHKPHLGKSSSLDKQLPGPSGGEEEKPMGNGSPSPPPGTSLDNPVPSPSPSEIFPVTHSFPSSAHSDTHH
  TSTAESQKKATAEGSAGRVENFGKRKPLLQAWVSPSETHPVSAQPGAGTGSAKHRLHPVKPMNATATKVA
  NCSLGTATIISENLNNEVMMKKYSPSDPAFAYAQLTHDELIQLVLKQKETISKKEFQVRELEDYIDNLLV
  RVMEETPNILRIPTQVGKKAGKM
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002814
• ORF Size: 3849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002814.1, NP_001002814.1
• RefSeq Size: 7856 bp
• RefSeq ORF: 3852 bp
• Locus ID: 80223
• UniProt ID: Q6WKZ4
• Cytogenetics: 8p11.23
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome. [provided by RefSeq, Dec 2013]