FCRL3 (NM_052939) Human Tagged ORF Clone

CAT#: RG214467

FCRL3 (tGFP-tagged) - Human Fc receptor-like 3 (FCRL3)

Product Images

Circular map for RG214467

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FCRL3
• Synonyms: CD307c; FCRH3; IFGP3; IRTA3; SPAP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214467 representing NM_052939
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCTGTGGCTGCTGCTGCTGATCCTGACTCCTGGAAGAGAACAATCAGGGGTGGCCCCAAAAGCTG
  TACTTCTCCTCAATCCTCCATGGTCCACAGCCTTCAAAGGAGAAAAAGTGGCTCTCATATGCAGCAGCAT
  ATCACATTCCCTAGCCCAGGGAGACACATATTGGTATCACGATGAGAAGTTGTTGAAAATAAAACATGAC
  AAGATCCAAATTACAGAGCCTGGAAATTACCAATGTAAGACCCGAGGATCCTCCCTCAGTGATGCCGTGC
  ATGTGGAATTTTCACCTGACTGGCTGATCCTGCAGGCTTTACATCCTGTCTTTGAAGGAGACAATGTCAT
  TCTGAGATGTCAGGGGAAAGACAACAAAAACACTCATCAAAAGGTTTACTACAAGGATGGAAAACAGCTT
  CCTAATAGTTATAATTTAGAGAAGATCACAGTGAATTCAGTCTCCAGGGATAATAGCAAATATCATTGTA
  CTGCTTATAGGAAGTTTTACATACTTGACATTGAAGTAACTTCAAAACCCCTAAATATCCAAGTTCAAGA
  GCTGTTTCTACATCCTGTGCTGAGAGCCAGCTCTTCCACGCCCATAGAGGGGAGTCCCATGACCCTGACC
  TGTGAGACCCAGCTCTCTCCACAGAGGCCAGATGTCCAGCTGCAATTCTCCCTCTTCAGAGATAGCCAGA
  CCCTCGGATTGGGCTGGAGCAGGTCCCCCAGACTCCAGATCCCTGCCATGTGGACTGAAGACTCAGGGTC
  TTACTGGTGTGAGGTGGAGACAGTGACTCACAGCATCAAAAAAAGGAGCCTGAGATCTCAGATACGTGTA
  CAGAGAGTCCCTGTGTCTAATGTGAATCTAGAGATCCGGCCCACCGGAGGGCAGCTGATTGAAGGAGAAA
  ATATGGTCCTTATTTGCTCAGTAGCCCAGGGTTCAGGGACTGTCACATTCTCCTGGCACAAAGAAGGAAG
  AGTAAGAAGCCTGGGTAGAAAGACCCAGCGTTCCCTGTTGGCAGAGCTGCATGTTCTCACCGTGAAGGAG
  AGTGATGCAGGGAGATACTACTGTGCAGCTGATAACGTTCACAGCCCCATCCTCAGCACGTGGATTCGAG
  TCACCGTGAGAATTCCGGTATCTCACCCTGTCCTCACCTTCAGGGCTCCCAGGGCCCACACTGTGGTGGG
  GGACCTGCTGGAGCTTCACTGTGAGTCCCTGAGAGGCTCTCCCCCGATCCTGTACCGATTTTATCATGAG
  GATGTCACCCTGGGGAACAGCTCAGCCCCCTCTGGAGGAGGAGCCTCCTTCAACCTCTCTCTGACTGCAG
  AACATTCTGGAAACTACTCCTGTGATGCAGACAATGGCCTGGGGGCCCAGCACAGTCATGGAGTGAGTCT
  CAGGGTCACAGTTCCGGTGTCTCGCCCCGTCCTCACCCTCAGGGCTCCCGGGGCCCAGGCTGTGGTGGGG
  GACCTGCTGGAGCTTCACTGTGAGTCCCTGAGAGGCTCCTTCCCGATCCTGTACTGGTTTTATCACGAGG
  ATGACACCTTGGGGAACATCTCGGCCCACTCTGGAGGAGGGGCATCCTTCAACCTCTCTCTGACTACAGA
  ACATTCTGGAAACTACTCATGTGAGGCTGACAATGGCCTGGGGGCCCAGCACAGTAAAGTGGTGACACTC
  AATGTTACAGGAACTTCCAGGAACAGAACAGGCCTTACCGCTGCGGGAATCACGGGGCTGGTGCTCAGCA
  TCCTCGTCCTTGCTGCTGCTGCTGCTCTGCTGCATTACGCCAGGGCCCGAAGGAAACCAGGAGGACTTTC
  TGCCACTGGAACATCTAGTCACAGTCCTAGTGAGTGTCAGGAGCCTTCCTCGTCCAGGCCTTCCAGGATA
  GACCCTCAAGAGCCCACTCACTCTAAACCACTAGCCCCAATGGAGCTGGAGCCAATGTACAGCAATGTAA
  ATCCTGGAGATAGCAACCCGATTTATTCCCAGATCTGGAGCATCCAGCATACAAAAGAAAACTCAGCTAA
  TTGTCCAATGATGCATCAAGAGCATGAGGAACTTACAGTCCTCTATTCAGAACTGAAGAAGACACACCCA
  GACGACTCTGCAGGGGAGGCTAGCAGCAGAGGCAGGGCCCATGAAGAAGATGATGAAGAAAACTATGAGA
  ATGTACCACGTGTATTACTGGCCTCAGACCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214467 representing NM_052939
  Red=Cloning site Green=Tags(s)
  
  MLLWLLLLILTPGREQSGVAPKAVLLLNPPWSTAFKGEKVALICSSISHSLAQGDTYWYHDEKLLKIKHD
  KIQITEPGNYQCKTRGSSLSDAVHVEFSPDWLILQALHPVFEGDNVILRCQGKDNKNTHQKVYYKDGKQL
  PNSYNLEKITVNSVSRDNSKYHCTAYRKFYILDIEVTSKPLNIQVQELFLHPVLRASSSTPIEGSPMTLT
  CETQLSPQRPDVQLQFSLFRDSQTLGLGWSRSPRLQIPAMWTEDSGSYWCEVETVTHSIKKRSLRSQIRV
  QRVPVSNVNLEIRPTGGQLIEGENMVLICSVAQGSGTVTFSWHKEGRVRSLGRKTQRSLLAELHVLTVKE
  SDAGRYYCAADNVHSPILSTWIRVTVRIPVSHPVLTFRAPRAHTVVGDLLELHCESLRGSPPILYRFYHE
  DVTLGNSSAPSGGGASFNLSLTAEHSGNYSCDADNGLGAQHSHGVSLRVTVPVSRPVLTLRAPGAQAVVG
  DLLELHCESLRGSFPILYWFYHEDDTLGNISAHSGGGASFNLSLTTEHSGNYSCEADNGLGAQHSKVVTL
  NVTGTSRNRTGLTAAGITGLVLSILVLAAAAALLHYARARRKPGGLSATGTSSHSPSECQEPSSSRPSRI
  DPQEPTHSKPLAPMELEPMYSNVNPGDSNPIYSQIWSIQHTKENSANCPMMHQEHEELTVLYSELKKTHP
  DDSAGEASSRGRAHEEDDEENYENVPRVLLASDH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052939
• ORF Size: 2202 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052939.4
• RefSeq Size: 3019 bp
• RefSeq ORF: 2205 bp
• Locus ID: 115352
• UniProt ID: Q96P31
• Cytogenetics: 1q23.1
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains immunoreceptor-tyrosine activation motifs and immunoreceptor-tyrosine inhibitory motifs in its cytoplasmic domain and may play a role in regulation of the immune system. Mutations in this gene have been associated with rheumatoid arthritis, autoimmune thyroid disease, and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]