HOMER2 (NM_199331) Human Tagged ORF Clone

CAT#: RG214311

HOMER2 (tGFP-tagged) - Human homer homolog 2 (Drosophila) (HOMER2), transcript variant 3

Product Images

Circular map for RG214311

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HOMER2
• Synonyms: ACPD; CPD; HOMER-2; HOMER2A; HOMER2B; Vesl-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214311 representing NM_199331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAACAGCCCATCTTCACCACCCGAGCGCATGTCTTCCAGATTGACCCCAACACCAAGAAGAACT
  GGATGCCTGCGAGCAAGCAGGCGGTCACCGTTTCCTACTTCTATGATGTCACAAGGAACAGCTATCGGAT
  CATCAGTGTGGACGGAGCCAAGGTGATCATAAACAGCACAATCACACCGAATATGACCTTCACCAAAACG
  TCACAGAAGTTTGGGCAGTGGGCCGACAGCAGAGCCAACACAGTGTTTGGTTTGGGGTTTTCCTCTGAGC
  AGCAGCTGACAAAGTTTGCAGAGAAATTCCAGGAGGTGAAAGAAGCTGCCAAGATAGCCAAAGACAAGAC
  GCAGGAGAAAATCGAGACCTCAAGTAATCATTCCCAAGCATCCAGTGTCAACGGGACGGACGATGAAAAG
  GCCTCTCACGCCGGTCCAGCCAACACACACCTGAAGTCTGAGAATGACAAGCTGAAGATTGCCTTGACGC
  AGAGCGCAGCCAACGTGAAGAATGAGATCAACAGAGAGAAGGAGAAGAACACGCAGCTGAAGAGGAGGAT
  CGAGGAGCTGGAGGCAGAGCTCCGAGAAAAGGAGACAGAGCTGAAAGATCTCCGAAAACAAAGTGAAATC
  ATACCTCAGCTCATGTCAGAGTGCGAATATGTCTCTGAGAAGCTAGAGGCGGCAGAGAGAGACAATCAAA
  ACCTGGAAGACAAAGTGCGTTCCTTAAAGACAGACATTGAGGAGAGCAAATACCGACAGCGCCACCTGAA
  GGTGGAGTTGAAGAGCTTCCTGGAGGTGCTGGACGGGAAGATTGACGACCTGCATGACTTCCGCCGAGGG
  CTCTCCAAGCTGGGCACCGATAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214311 representing NM_199331
  Red=Cloning site Green=Tags(s)
  
  MGEQPIFTTRAHVFQIDPNTKKNWMPASKQAVTVSYFYDVTRNSYRIISVDGAKVIINSTITPNMTFTKT
  SQKFGQWADSRANTVFGLGFSSEQQLTKFAEKFQEVKEAAKIAKDKTQEKIETSSNHSQASSVNGTDDEK
  ASHAGPANTHLKSENDKLKIALTQSAANVKNEINREKEKNTQLKRRIEELEAELREKETELKDLRKQSEI
  IPQLMSECEYVSEKLEAAERDNQNLEDKVRSLKTDIEESKYRQRHLKVELKSFLEVLDGKIDDLHDFRRG
  LSKLGTDN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199331
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199331.2, NP_955363.2
• RefSeq Size: 1807 bp
• RefSeq ORF: 866 bp
• Locus ID: 9455
• Cytogenetics: 15q25.2
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. The encoded protein is a postsynaptic density scaffolding protein. Alternative splicing results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 14. [provided by RefSeq, Jun 2011]