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Treacher Collins syndrome protein (TCOF1) (NM_000356) Human Tagged ORF Clone
CAT#: RG213593
- TrueORF®
TCOF1 (tGFP-tagged) - Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 2
ORF Plasmid: DDK
"NM_000356" in other vectors (2)
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Other products for "Treacher Collins syndrome protein"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | Treacher Collins syndrome protein |
| Synonyms | MFD1; TCS; TCS1; treacle |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213593 representing NM_000356
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCCAGGAAGCGGCGGGAGCTACTTCCCCTGATCTACCACCATCTGCTGCGGGCTGGCTATG TGCGTGCGGCGCGGGAAGTGAAGGAGCAGAGCGGCCAGAAGTGTTTCCTGGCTCAGCCCGTAACCCTTCT GGACATCTATACACACTGGCAACAAACCTCAGAGCTTGGTCGGAAGCGGAAGGCAGAGGAAGATGCGGCA CTGCAAGCTAAGAAAACCCGTGTGTCAGACCCCATCAGCACCTCGGAGAGCTCGGAAGAGGAGGAAGAAG CAGAAGCCGAAACCGCCAAAGCCACCCCAAGACTAGCATCTACCAACTCCTCAGTCCTGGGGGCGGACTT GCCATCAAGCATGAAAGAAAAAGCCAAGGCAGAGACAGAGAAAGCTGGCAAGACTGGGAATTCCATGCCA CACCCTGCCACTGGGAAGACGGTGGCCAACCTTCTTTCTGGGAAGTCTCCCAGGAAGTCAGCAGAGCCCT CAGCAAATACTACGTTGGTCTCAGAAACTGAGGAGGAGGGCAGCGTCCCGGCCTTTGGAGCTGCTGCCAA GCCTGGGATGGTGTCAGCGGGCCAGGCCGACAGCTCCAGCGAGGACACCTCCAGCTCCAGTGATGAGACA GACGTGGAGGTAAAGGCCTCTGAAAAAATTCTCCAGGTCAGAGCTGCCTCAGCCCCTGCCAAGGGGACCC CTGGGAAAGGGGCTACCCCAGCACCCCCTGGGAAGGCAGGGGCTGTAGCCTCCCAGACCAAGGCAGGGAA GCCAGAGGAGGACTCAGAGAGCAGCAGCGAGGAGTCATCTGACAGTGAGGAGGAGACGCCAGCTGCCAAG GCCCTGCTTCAGGCGAAGGCCTCAGGAAAAACCTCTCAGGTCGGAGCTGCCTCAGCCCCTGCCAAGGAGT CCCCCAGGAAAGGAGCTGCCCCAGCGCCCCCTGGGAAGACAGGGCCTGCAGTTGCCAAGGCCCAGGCGGG GAAGCGGGAGGAGGACTCGCAGAGCAGCAGCGAGGAATCGGACAGTGAGGAGGAGGCGCCTGCTCAGGCG AAGCCTTCAGGGAAGGCCCCCCAGGTCAGAGCCGCCTCGGCCCCTGCCAAGGAGTCCCCCAGGAAAGGGG CTGCCCCAGCACCTCCTAGGAAAACAGGGCCTGCAGCCGCCCAGGTCCAGGTGGGGAAGCAGGAGGAGGA CTCAAGAAGCAGCAGCGAGGAGTCAGACAGTGACAGAGAGGCACTGGCAGCCATGAATGCAGCTCAGGTG AAGCCCTTGGGGAAAAGCCCCCAGGTGAAACCTGCCTCTACCATGGGCATGGGGCCCTTGGGGAAAGGCG CCGGCCCAGTGCCACCCGGGAAGGTGGGGCCTGCAACCCCCTCAGCCCAGGTGGGGAAGTGGGAGGAGGA CTCAGAGAGCAGTAGTGAGGAGTCATCAGACAGCAGTGATGGAGAGGTGCCCACAGCTGTGGCCCCGGCT CAGGAAAAGTCCTTGGGGAACATCCTCCAGGCCAAACCCACCTCCAGTCCTGCCAAGGGGCCCCCTCAGA AGGCAGGGCCTGTAGCCGTCCAGGTCAAGGCTGAAAAGCCCATGGACAACTCGGAGAGCAGCGAGGAGTC ATCGGACAGTGCGGACAGTGAGGAGGCACCAGCAGCCATGACTGCAGCTCAGGCAAAACCAGCTCTGAAA ATTCCTCAGACCAAGGCCTGCCCAAAGAAAACCAATACCACTGCATCTGCCAAGGTCGCCCCTGTGCGAG TGGGCACCCAAGCCCCCCGGAAAGCAGGAACTGCGACTTCTCCAGCAGGCTCATCCCCAGCTGTGGCTGG GGGCACCCAGAGACCAGCAGAGGATTCTTCAAGCAGTGAGGAATCAGATAGTGAGGAAGAGAAGACAGGT CTTGCAGTAACCGTGGGACAGGCAAAGTCTGTGGGGAAAGGCCTCCAGGTGAAAGCAGCCTCAGTGCCTG TCAAGGGGTCCTTGGGGCAAGGGACTGCTCCAGTACTCCCTGGGAAGACGGGGCCTACAGTCACCCAGGT GAAAGCTGAAAAGCAGGAAGACTCTGAGAGCAGTGAGGAGGAATCAGACAGTGAGGAAGCAGCTGCATCT CCAGCACAGGTGAAAACCTCAGTAAAGAAAACCCAGGCCAAAGCCAACCCAGCTGCCGCCAGAGCACCTT CAGCAAAAGGGACAATTTCAGCCCCTGGAAAAGTTGTCACTGCAGCTGCTCAAGCCAAGCAGAGGTCTCC ATCCAAGGTGAAGCCACCAGTGAGAAACCCCCAGAACAGTACCGTCTTGGCGAGGGGCCCAGCATCTGTG CCATCTGTGGGGAAGGCCGTGGCTACAGCAGCTCAGGCCCAGACAGGGCCAGAGGAGGACTCAGGGAGCA GTGAGGAGGAGTCAGACAGTGAGGAGGAGGCGGAGACGCTGGCTCAGGTGAAGCCTTCAGGGAAGACCCA CCAGATCAGAGCTGCCTTGGCTCCTGCCAAGGAGTCCCCCAGGAAAGGGGCTGCCCCAACACCTCCTGGG AAGACAGGGCCTTCGGCTGCCCAGGCAGGGAAGCAGGATGACTCAGGGAGCAGCAGCGAGGAATCAGACA GTGATGGGGAGGCACCGGCAGCTGTGACCTCTGCCCAGGTGATTAAACCCCCTCTGATTTTTGTCGACCC TAATCGTAGTCCAGCTGGCCCAGCTGCTACACCCGCACAAGCCCAGGCTGCAAGCACCCCGAGGAAGGCC CGAGCCTCGGAGAGCACAGCCAGGAGCTCCTCCTCCGAGAGCGAGGATGAGGACGTGATCCCCGCTACAC AGTGCTTGACTCCTGGCATCAGAACCAATGTGGTGACCATGCCCACTGCCCACCCAAGAATAGCCCCCAA AGCCAGCATGGCTGGGGCCAGCAGCAGCAAGGAGTCCAGTCGGATATCAGATGGCAAGAAACAGGAGGGA CCAGCCACTCAGGTGTCAAAGAAGAACCCAGCTTCCCTCCCACTGACCCAGGCTGCCCTGAAGGTCCTCG CCCAGAAAGCCAGTGAGGCTCAGCCTCCTGTTGCCAGGACCCAGCCTTCAAGTGGGGTTGACAGTGCTGT GGGAACACTCCCTGCAACAAGTCCCCAGAGCACCTCCGTCCAGGCCAAAGGGACCAACAAGCTCAGAAAA CCTAAGCTTCCTGAGGTCCAGCAGGCCACCAAAGCCCCTGAGAGCTCAGATGACAGTGAGGACAGCAGCG ACAGTTCTTCAGGGAGTGAGGAAGATGGTGAAGGGCCCCAGGGGGCCAAGTCAGCCCACACGCTGGGTCC CACCCCCTCCAGGACAGAGACCCTGGTGGAGGAGACCGCAGCAGAGTCCAGCGAGGATGATGTGGTGGCG CCATCCCAGTCTCTCCTCTCAGGTTATATGACCCCTGGACTAACCCCAGCCAATTCCCAGGCCTCAAAAG CCACTCCCAAGCTAGACTCCAGCCCCTCAGTTTCCTCTACTCTGGCCGCCAAAGATGACCCAGATGGCAA GCAGGAGGCAAAGCCCCAACAGGCAGCAGGCATGTTGTCCCCTAAAACAGGTGGAAAAGAGGCTGCTTCA GGCACCACACCTCAGAAGTCCCGGAAGCCCAAGAAAGGGGCTGGGAACCCCCAAGCCTCAACCCTGGCGC TGCAAAGCAACATCACCCAGTGCCTCCTGGGCCAACCCTGGCCCCTGAATGAGGCCCAGGTGCAGGCCTC AGTGGTGAAGGTCCTGACTGAGCTGCTGGAACAGGAAAGAAAGAAGGTGGTGGACACCACCAAGGAGAGC AGCAGGAAGGGCTGGGAGAGCCGCAAGCGGAAGCTATCGGGAGACCAGCCAGCTGCCAGGACCCCCAGGA GCAAGAAGAAGAAGAAGCTGGGGGCCGGGGAAGGTGGGGAGGCCTCTGTTTCCCCAGAAAAGACCTCCAC GACTTCCAAGGGGAAAGCAAAGAGAGACAAAGCAAGTGGTGATGTCAAGGAGAAGAAAGGGAAGGGGTCT CTTGGCTCCCAAGGGGCCAAGGACGAGCCAGAAGAGGAGCTTCAGAAGGGGATGGGGACGGTTGAAGGTG GAGATCAAAGCAACCCAAAGAGCAAGAAGGAGAAGAAGAAATCCGACAAGAGAAAAAAAGACAAAGAAAA AAAAGAAAAGAAGAAGAAAGCAAAAAAGGCCTCAACCAAAGATTCTGAGTCACCGTCCCAGAAGAAAAAG AAGAAAAAGAAGAAGACAGCAGAGCAGACTGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213593 representing NM_000356
Red=Cloning site Green=Tags(s) MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAA LQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMP HPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDET DVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAK ALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQA KPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQV KPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPA QEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALK IPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTG LAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAAS PAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASV PSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPG KTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKA RASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEG PATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRK PKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVA PSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAAS GTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKES SRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGS LGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKK KKKKKTAEQTV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000356 |
| ORF Size | 4233 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000356.4 |
| RefSeq Size | 4882 bp |
| RefSeq ORF | 4236 bp |
| Locus ID | 6949 |
| UniProt ID | Q13428 |
| Cytogenetics | 5q32-q33.1 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Gene Summary | This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
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