PCTAIRE3 (CDK18) (NM_212502) Human Tagged ORF Clone

CAT#: RG213327

CDK18 (tGFP-tagged) - Human cyclin-dependent kinase 18 (CDK18), transcript variant 2

Product Images

Circular map for RG213327

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCTAIRE3
• Synonyms: PCTAIRE; PCTAIRE3; PCTK3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213327 representing NM_212502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAGATGAAGAACTTTAAGCGCCGTTTCTCCCTGTCAGTGCCCCGCACTGAGACCATTGAAGAAT
  CCTTGGCTGAATTCACGGAGCAATTCAACCAGCTCCACAACCGGCGGAATGAGAACTTGCAGCTCGGTCC
  TCTTGGCAGAGACCCCCCGCAGGAGTGCAGCACCTTCTCCCCAACAGACAGCGGGGAGGAGCCGGGGCAG
  CTCTCCCCTGGCGTGCAGTTCCAGCGGCGGCAGAACCAGCGCCGCTTCTCCATGGAGGACGTCAGCAAGA
  GGCTCTCTCTGCCCATGGATATCCGCCTGCCCCAGGAATTCCTACAGAAGCTACAGATGGAGAGCCCAGA
  TCTGCCCAAGCCGCTCAGCCGCATGTCCCGCCGGGCCTCCCTGTCAGACATTGGCTTTGGGAAACTGGAA
  ACATACGTGAAACTGGACAAACTGGGAGAGGGCACCTATGCCACAGTCTTCAAAGGGCGCAGCAAACTGA
  TGGAGAACCTTGTGGCCCTGAAAGAGATCCGGCTGGAGCACGAGGAGGGAGCGCCCTGCACTGCCATCCG
  AGAGGTGTCTCTGCTGAAGAACCTGAAGCACGCCAATATTGTGACCCTGCATGACCTCATCCACACAGAT
  CGGTCCCTCACCCTGGTGTTTGAGTACCTGGACAGTGACCTGAAGCAGTATCTGGACCACTGTGGGAACC
  TCATGAGCATGCACAACGTCAAGATTTTCATGTTCCAGCTGCTCCGGGGCCTCGCCTACTGTCACCACCG
  CAAGATCCTGCACCGGGACCTGAAGCCCCAGAACCTGCTCATCAACGAGAGGGGGGAGCTGAAGCTGGCC
  GACTTTGGACTGGCCAGGGCCAAGTCAGTGCCCACAAAGACTTACTCCAATGAGGTGGTGACCCTGTGGT
  ACAGGCCCCCCGATGTGCTGCTGGGATCCACAGAGTACTCCACCCCCATTGATATGTGGGGCGTGGGCTG
  CATCCACTACGAGATGGCCACAGGGAGGCCCCTCTTCCCGGGCTCCACAGTCAAGGAGGAGCTGCACCTC
  ATCTTTCGCCTCCTCGGGACCCCCACAGAAGAGACGTGGCCCGGCGTGACCGCCTTCTCTGAGTTCCGCA
  CCTACAGCTTCCCCTGCTACCTCCCGCAGCCGCTCATCAACCACGCGCCCAGGTTGGATACGGATGGCAT
  CCACCTCCTGAGCAGCCTGCTCCTGTATGAATCCAAGAGTCGCATGTCAGCAGAGGCTGCCCTGAGTCAC
  TCCTACTTCCGGTCTCTGGGAGAGCGTGTGCACCAGCTTGAAGACACTGCCTCCATCTTCTCCCTGAAGG
  AGATCCAGCTCCAGAAGGACCCAGGCTACCGAGGCTTGGCCTTCCAGCAGCCAGGACGAGGGAAGAACAG
  GCGGCAGAGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213327 representing NM_212502
  Red=Cloning site Green=Tags(s)
  
  MNKMKNFKRRFSLSVPRTETIEESLAEFTEQFNQLHNRRNENLQLGPLGRDPPQECSTFSPTDSGEEPGQ
  LSPGVQFQRRQNQRRFSMEDVSKRLSLPMDIRLPQEFLQKLQMESPDLPKPLSRMSRRASLSDIGFGKLE
  TYVKLDKLGEGTYATVFKGRSKLMENLVALKEIRLEHEEGAPCTAIREVSLLKNLKHANIVTLHDLIHTD
  RSLTLVFEYLDSDLKQYLDHCGNLMSMHNVKIFMFQLLRGLAYCHHRKILHRDLKPQNLLINERGELKLA
  DFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGSTEYSTPIDMWGVGCIHYEMATGRPLFPGSTVKEELHL
  IFRLLGTPTEETWPGVTAFSEFRTYSFPCYLPQPLINHAPRLDTDGIHLLSSLLLYESKSRMSAEAALSH
  SYFRSLGERVHQLEDTASIFSLKEIQLQKDPGYRGLAFQQPGRGKNRRQSIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212502
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212502.3
• RefSeq Size: 3075 bp
• RefSeq ORF: 1425 bp
• Locus ID: 5129
• UniProt ID: Q07002
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: May play a role in signal transduction cascades in terminally differentiated cells.[UniProtKB/Swiss-Prot Function]