LGICZ1 (ZACN) (NM_180990) Human Tagged ORF Clone

CAT#: RG212824

ZACN (tGFP-tagged) - Human zinc activated ligand-gated ion channel (ZACN)

Product Images

Circular map for RG212824

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LGICZ1
• Synonyms: L2; LGICZ; LGICZ1; ZAC; ZAC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212824 representing NM_180990
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTATGGTCCCTGCTCCATCTCACCTTCCTGGGGTTCAGCATTACCTTGCTGTTGGTCCACGGGC
  AGGGCTTCCAAGGGACAGCAGCCATCTGGCCATCCCTCTTCAACGTCAACTTGTCCAAGAAGGTTCAGGA
  AAGCATCCAGATCCCGAACAATGGGAGTGCGCCCCTGCTCGTGGATGTGCGGGTGTTTGTCTCCAACGTG
  TTTAATGTGGACATCCTGCGATACACAATGTCCTCCATGCTGCTGCTTAGGCTGTCCTGGCTGGACACTC
  GCCTGGCCTGGAACACTAGTGCACACCCGCGGCACGCCATCACGCTGCCCTGGGAGTCTCTCTGGACACC
  AAGGCTCACCATCCTGGAGGCGCTCTGGGTGGACTGGAGGGACCAGAGCCCCCAGGCTCGAGTAGACCAG
  GACGGCCACGTGAAGCTCAACCTGGCCCTCACCACGGAGACCAACTGCAACTTTGAGCTCCTCCACTTCC
  CCCGGGACCACAGCAACTGCAGCCTCAGCTTCTACGCTCTCAGCAACACGGCGATGGAGTTAGAGTTCCA
  GGCCCACGTGGTGAACGAGATTGTGAGTGTCAAGAGGGAATACGTAGTTTATGATCTGAAGACCCAAGTC
  CCACCCCAGCAGCTGGTGCCCTGCTTCCAGGTGACGCTGAGGCTGAAGAACACGGCGCTCAAGTCCATCA
  TCGCTCTCTTGGTGCCTGCAGAGGCACTGCTGTTGGCTGACGTGTGCGGGGGGTTGCTGCCCCTCCGGGC
  CATTGAGCGCATAGGCTACAAGGTGACATTGCTGCTGAGTTACCTCGTCCTCCACTCCTCCCTGGTGCAG
  GCCCTGCCCAGCTCCTCCTCCTGCAACCCACTGCTCATTTACTACTTCACCATCCTGCTGCTGCTGCTCT
  TCCTCAGCACCATAGAGACTGTGCTGCTGGCTGGGCTGCTGGCCCGGGGCAACCTTGGGGCCAAGAGCGG
  CCCCAGCCCAGCCCCGAGAGGGGAACAGCGAGAGCACGGCAACCCAGGGCCTCATCCTGCTGAAGAGCCC
  TCCAGAGGAGTAAAGGGGTCACAGAGAAGCTGGCCTGAGACTGCTGACCGCATCTTCTTCCTCGTGTATG
  TGGTTGGGGTGCTGTGCACCCAATTCGTCTTTGCAGGAATCTGGATGTGGGCAGCGTGCAAGTCTGACGC
  AGCCCCTGGAGAGGCTGCACCCCATGGCAGGCGGCCTAGACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212824 representing NM_180990
  Red=Cloning site Green=Tags(s)
  
  MALWSLLHLTFLGFSITLLLVHGQGFQGTAAIWPSLFNVNLSKKVQESIQIPNNGSAPLLVDVRVFVSNV
  FNVDILRYTMSSMLLLRLSWLDTRLAWNTSAHPRHAITLPWESLWTPRLTILEALWVDWRDQSPQARVDQ
  DGHVKLNLALTTETNCNFELLHFPRDHSNCSLSFYALSNTAMELEFQAHVVNEIVSVKREYVVYDLKTQV
  PPQQLVPCFQVTLRLKNTALKSIIALLVPAEALLLADVCGGLLPLRAIERIGYKVTLLLSYLVLHSSLVQ
  ALPSSSSCNPLLIYYFTILLLLLFLSTIETVLLAGLLARGNLGAKSGPSPAPRGEQREHGNPGPHPAEEP
  SRGVKGSQRSWPETADRIFFLVYVVGVLCTQFVFAGIWMWAACKSDAAPGEAAPHGRRPRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_180990
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_180990.4
• RefSeq Size: 1289 bp
• RefSeq ORF: 1239 bp
• Locus ID: 353174
• UniProt ID: Q401N2
• Cytogenetics: 17q25.1
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: LGICZ1 is a zinc-activated ligand-gated ion channel that defines a new subgroup of the cysteine-loop superfamily of ligand-gated ion channels (Davies et al., 2003 [PubMed 12381728]).[supplied by OMIM, Mar 2008]