PCDH7 (NM_032456) Human Tagged ORF Clone

CAT#: RG212012

PCDH7 (tGFP-tagged) - Human protocadherin 7 (PCDH7), transcript variant b

Product Images

Circular map for RG212012

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCDH7
• Synonyms: BH-Pcdh; BHPCDH; PPP1R120
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212012 representing NM_032456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGATGCGGACCGCGGGATGGGCGCGCGGCTGGTGCTTGGGCTGCTGCCTCCTCCTGCCGCTCT
  CGCTCAGCCTGGCGGCCGCCAAGCAGCTCCTCCGGTACCGGCTGGCCGAGGAGGGCCCCGCCGACGTCCG
  CATCGGCAACGTGGCTTCAGACCTGGGCATCGTGACCGGATCGGGTGAGGTGACTTTCAGCCTGGAGTCC
  GGTTCCGAGTACCTGAAGATCGACAACCTCACTGGCGAGCTGAGCACGAGCGAGCGGCGCATCGACCGCG
  AGAAGCTGCCCCAGTGTCAGATGATCTTCGACGAGAACGAGTGCTTCCTGGACTTCGAGGTGTCGGTGAT
  CGGGCCCTCGCAGAGCTGGGTGGACCTGTTTGAGGGTCAGGTCATCGTGCTTGACATCAACGACAACACG
  CCCACCTTCCCGTCGCCCGTGCTCACGCTCACGGTGGAGGAGAATCGGCCGGTGGGCACACTTTACCTGC
  TGCCCACAGCCACCGACCGCGACTTCGGCCGCAACGGCATCGAGCGCTACGAGCTGCTCCAGGAGCCCGG
  AGGCGGCGGCAGCGGCGGCGAGAGCCGGCGCGCCGGGGCGGCCGACAGCGCCCCCTACCCCGGGGGCGGC
  GGGAACGGCGCGAGCGGCGGCGGCTCGGGAGGCTCCAAGCGGCGGCTGGACGCATCAGAGGGCGGCGGCG
  GCACCAACCCCGGCGGCCGCAGCAGCGTGTTCGAGCTGCAGGTGGCGGACACCCCGGACGGCGAGAAGCA
  GCCGCAGCTGATCGTGAAGGGGGCGCTGGACCGCGAGCAGCGCGACTCCTACGAGCTGACCCTGCGAGTG
  CGCGACGGCGGCGACCCGCCTCGCTCCTCGCAGGCCATCCTACGGGTCCTCATCACCGACGTGAACGACA
  ACAGCCCCCGCTTCGAGAAGAGCGTGTACGAGGCCGACTTGGCTGAGAACAGCGCCCCGGGGACCCCCAT
  CCTGCAACTGCGCGCAGCCGACTTGGACGTGGGGGTCAACGGGCAGATCGAATACGTGTTCGGGGCGGCC
  ACCGAGTCGGTGAGGCGGCTGCTGCGCCTTGACGAGACGTCCGGCTGGCTCAGCGTCCTGCACCGGATCG
  ACCGCGAGGAGGTGAACCAGCTGCGCTTCACGGTCATGGCCCGCGACCGCGGGCAGCCCCCCAAGACCGA
  CAAGGCCACCGTGGTCCTTAACATCAAAGACGAGAACGACAACGTGCCGTCCATTGAAATCCGCAAGATT
  GGGCGCATCCCCCTCAAGGACGGGGTGGCCAACGTGGCCGAGGACGTTCTGGTCGACACCCCCATCGCTC
  TGGTGCAGGTGTCCGACCGAGACCAAGGCGAGAACGGGGTGGTCACCTGCACCGTGGTGGGCGACGTGCC
  CTTCCAGCTCAAGCCAGCCAGCGACACCGAGGGCGACCAGAACAAGAAAAAGTACTTCTTGCACACCTCG
  ACCCCTCTGGACTATGAGGCCACCCGGGAGTTCAACGTGGTCATCGTGGCGGTGGACTCAGGCAGCCCCA
  GCCTCTCGAGCAACAACTCCCTGATTGTCAAGGTGGGAGACACCAACGACAACCCGCCCATGTTCGGCCA
  GTCGGTGGTGGAGGTTTACTTCCCTGAGAACAACATCCCGGGCGAGAGGGTGGCCACGGTGCTGGCGACA
  GACGCAGACAGCGGTAAGAACGCCGAGATCGCCTACTCGCTGGACTCCTCTGTGATGGGGATCTTTGCCA
  TCGATCCCGATTCTGGGGACATCCTGGTCAATACCGTGCTGGACCGCGAGCAGACTGACAGGTATGAGTT
  TAAAGTTAACGCCAAAGACAAAGGCATCCCCGTGCTGCAGGGCAGCACTACGGTGATTGTGCAGGTGGCT
  GATAAAAATGACAATGACCCTAAGTTTATGCAGGACGTCTTCACCTTTTATGTGAAAGAAAACTTGCAGC
  CCAACAGCCCTGTGGGGATGGTCACCGTGATGGATGCTGACAAGGGGCGGAATGCAGAGATGAGCCTGTA
  CATAGAGGAGAACAATAACATTTTTTCTATTGAAAATGACACGGGGACCATTTACTCCACAATGTCTTTT
  GACCGGGAACATCAGACCACATACACTTTCAGAGTCAAGGCTGTGGATGGGGGAGATCCTCCCAGATCTG
  CCACAGCTACAGTCTCGCTTTTTGTGATGGATGAAAATGACAATGCTCCCACAGTTACCCTTCCCAAAAA
  CATTTCCTACACTTTACTGCCACCTTCGAGTAATGTCAGGACAGTAGTAGCTACAGTGTTGGCAACAGAC
  AGTGATGATGGCATCAATGCAGACCTGAACTACAGCATTGTGGGAGGAAATCCCTTCAAGCTGTTTGAAA
  TTGATCCCACTAGTGGTGTGGTTTCCTTAGTGGGAAAACTCACCCAAAAGCATTATGGCTTGCACAGGTT
  GGTGGTGCAAGTGAATGACAGTGGGCAGCCTTCCCAGTCCACCACGACTCTGGTGCACGTGTTTGTCAAT
  GAAAGTGTTTCTAATGCAACTGCGATTGACTCCCAGATAGCTAGAAGTTTGCACATCCCACTCACCCAGG
  ATATAGCTGGTGACCCAAGCTATGAAATTAGCAAACAGAGACTCAGTATTGTCATTGGCGTGGTTGCTGG
  CATTATGACGGTGATTCTAATCATCTTAATTGTAGTGATGGCAAGGTACTGCAGGTCCAAAAATAAAAAT
  GGCTATGAAGCCGGCAAAAAAGATCACGAAGACTTTTTTACACCCCAACAGCATGACAAATCTAAAAAGC
  CTAAAAAGGACAAGAAAAACAAAAAATCTAAGCAGCCTCTCTACAGCAGCATTGTCACTGTGGAGGCTTC
  TAAGCCAAATGGACAGAGGTATGATAGTGTCAATGAGAAGCTGTCAGACAGCCCAAGCATGGGGCGATAC
  AGGTCCGTTAATGGTGGGCCCGGCAGTCCTGACCTGGCAAGGCATTACAAATCTAGTTCCCCATTGCCTA
  CTGTTCAGCTTCATCCCCAGTCACCAACTGCAGGAAAAAAACACCAGGCCGTACAAGATCTACCACCAGC
  CAACACATTTGTGGGAGCAGGAGACAACATTTCAATTGGATCAGATCACTGCTCTGAGTACAGCTGTCAA
  ACCAATAACAAGTACAGCAAACAGGTAAGATGTATCCCAAATATATTTAAATATCCCAGGGAGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212012 representing NM_032456
  Red=Cloning site Green=Tags(s)
  
  MLRMRTAGWARGWCLGCCLLLPLSLSLAAAKQLLRYRLAEEGPADVRIGNVASDLGIVTGSGEVTFSLES
  GSEYLKIDNLTGELSTSERRIDREKLPQCQMIFDENECFLDFEVSVIGPSQSWVDLFEGQVIVLDINDNT
  PTFPSPVLTLTVEENRPVGTLYLLPTATDRDFGRNGIERYELLQEPGGGGSGGESRRAGAADSAPYPGGG
  GNGASGGGSGGSKRRLDASEGGGGTNPGGRSSVFELQVADTPDGEKQPQLIVKGALDREQRDSYELTLRV
  RDGGDPPRSSQAILRVLITDVNDNSPRFEKSVYEADLAENSAPGTPILQLRAADLDVGVNGQIEYVFGAA
  TESVRRLLRLDETSGWLSVLHRIDREEVNQLRFTVMARDRGQPPKTDKATVVLNIKDENDNVPSIEIRKI
  GRIPLKDGVANVAEDVLVDTPIALVQVSDRDQGENGVVTCTVVGDVPFQLKPASDTEGDQNKKKYFLHTS
  TPLDYEATREFNVVIVAVDSGSPSLSSNNSLIVKVGDTNDNPPMFGQSVVEVYFPENNIPGERVATVLAT
  DADSGKNAEIAYSLDSSVMGIFAIDPDSGDILVNTVLDREQTDRYEFKVNAKDKGIPVLQGSTTVIVQVA
  DKNDNDPKFMQDVFTFYVKENLQPNSPVGMVTVMDADKGRNAEMSLYIEENNNIFSIENDTGTIYSTMSF
  DREHQTTYTFRVKAVDGGDPPRSATATVSLFVMDENDNAPTVTLPKNISYTLLPPSSNVRTVVATVLATD
  SDDGINADLNYSIVGGNPFKLFEIDPTSGVVSLVGKLTQKHYGLHRLVVQVNDSGQPSQSTTTLVHVFVN
  ESVSNATAIDSQIARSLHIPLTQDIAGDPSYEISKQRLSIVIGVVAGIMTVILIILIVVMARYCRSKNKN
  GYEAGKKDHEDFFTPQQHDKSKKPKKDKKNKKSKQPLYSSIVTVEASKPNGQRYDSVNEKLSDSPSMGRY
  RSVNGGPGSPDLARHYKSSSPLPTVQLHPQSPTAGKKHQAVQDLPPANTFVGAGDNISIGSDHCSEYSCQ
  TNNKYSKQVRCIPNIFKYPREG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032456
• ORF Size: 3216 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032456.3
• RefSeq Size: 4928 bp
• RefSeq ORF: 3219 bp
• Locus ID: 5099
• UniProt ID: O60245
• Cytogenetics: 4p15.1
• Domains: CA
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008]