CHRNA9 (NM_017581) Human Tagged ORF Clone

CAT#: RG211880

CHRNA9 (tGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 9 (CHRNA9)

Product Images

Circular map for RG211880

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHRNA9
• Synonyms: HSA243342; NACHRA9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211880 representing NM_017581
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTGGTCCCATTCCTGCATCTCCTTTTGCTGGATCTACTTTGCTGCTTCCAGACTGAGAGCTGCAG
  AGACGGCAGATGGAAAATATGCTCAGAAGTTGTTTAATGACCTTTTTGAAGATTATTCTAATGCTCTTCG
  TCCAGTGGAAGATACAGATAAAGTCCTGAATGTGACCCTGCAGATTACGCTCTCTCAGATTAAGGATATG
  GATGAAAGAAACCAAATTCTGACTGCTTATTTGTGGATCCGCCAAATCTGGCACGATGCCTATCTCACGT
  GGGACCGAGATCAGTACGATGGCCTAGACTCCATCAGGATCCCCAGTGACCTCGTGTGGAGGCCAGACAT
  CGTCTTATATAACAAGGCTGATGATGAATCTTCAGAGCCTGTGAACACCAATGTGGTCCTGCGGTATGAT
  GGGCTGATCACCTGGGATGCACCGGCCATCACCAAAAGCTCCTGTGTGGTGGATGTCACCTACTTCCCTT
  TTGACAACCAGCAGTGCAACCTGACTTTTGGTTCCTGGACCTACAATGGCAATCAGGTGGACATATTCAA
  CGCCTTGGACAGCGGAGATCTCTCTGACTTCATTGAAGATGTGGAATGGGAGGTCCATGGCATGCCCGCT
  GTGAAGAATGTGATCTCCTATGGCTGCTGCTCTGAGCCTTACCCGGATGTCACATTCACCCTCCTTCTGA
  AGAGGAGGTCCTCGTTCTATATCGTCAACCTCCTCATCCCATGCGTCCTCATATCTTTTCTGGCTCCTCT
  GAGTTTTTATCTCCCAGCAGCCTCCGGAGAAAAGGTCTCCCTGGGAGTGACCATCCTGTTGGCCATGACT
  GTATTTCAGCTAATGGTGGCAGAAATCATGCCGGCCTCAGAAAATGTGCCCCTGATAGGTAAATACTACA
  TAGCCACGATGGCCCTGATCACAGCCTCCACTGCGTTGACCATCATGGTGATGAATATCCACTTCTGTGG
  GGCCGAGGCCCGGCCGGTGCCACACTGGGCCAGGGTGGTCATCCTGAAATACATGTCCAGGGTCTTGTTT
  GTCTATGATGTGGGTGAAAGCTGCCTCAGCCCGCACCACAGTAGAGAGCGGGACCACCTCACGAAAGTTT
  ATAGCAAACTCCCAGAGTCTAACCTGAAAGCAGCCAGGAACAAAGACCTTTCCAGAAAGAAGGACATGAA
  CAAACGCTTAAAGAACGACCTGGGCTGCCAGGGTAAGAACCCTCAGGAGGCCGAGAGTTACTGTGCACAG
  TACAAAGTGCTGACGAGGAATATTGAGTACATCGCCAAGTGCCTCAAAGACCACAAGGCCACCAGTTCCA
  AGGGGAGTGAATGGAAGAAGGTGGCGAAAGTCATAGACCGATTCTTCATGTGGATTTTTTTCATTATGGT
  GTTTGTGATGACTATTTTGATCATAGCAAGAGCGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211880 representing NM_017581
  Red=Cloning site Green=Tags(s)
  
  MNWSHSCISFCWIYFAASRLRAAETADGKYAQKLFNDLFEDYSNALRPVEDTDKVLNVTLQITLSQIKDM
  DERNQILTAYLWIRQIWHDAYLTWDRDQYDGLDSIRIPSDLVWRPDIVLYNKADDESSEPVNTNVVLRYD
  GLITWDAPAITKSSCVVDVTYFPFDNQQCNLTFGSWTYNGNQVDIFNALDSGDLSDFIEDVEWEVHGMPA
  VKNVISYGCCSEPYPDVTFTLLLKRRSSFYIVNLLIPCVLISFLAPLSFYLPAASGEKVSLGVTILLAMT
  VFQLMVAEIMPASENVPLIGKYYIATMALITASTALTIMVMNIHFCGAEARPVPHWARVVILKYMSRVLF
  VYDVGESCLSPHHSRERDHLTKVYSKLPESNLKAARNKDLSRKKDMNKRLKNDLGCQGKNPQEAESYCAQ
  YKVLTRNIEYIAKCLKDHKATSSKGSEWKKVAKVIDRFFMWIFFIMVFVMTILIIARAD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017581
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017581.1
• RefSeq Size: 1892 bp
• RefSeq ORF: 1440 bp
• Locus ID: 55584
• UniProt ID: Q9UGM1
• Cytogenetics: 4p14
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: This gene is a member of the ligand-gated ionic channel family and nicotinic acetylcholine receptor gene superfamily. It encodes a plasma membrane protein that forms homo- or hetero-oligomeric divalent cation channels. This protein is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea. [provided by RefSeq, Feb 2012]