ANKS1B (NM_181670) Human Tagged ORF Clone

CAT#: RG211877

ANKS1B (tGFP-tagged) - Human ankyrin repeat and sterile alpha motif domain containing 1B (ANKS1B), transcript variant 2

Product Images

Circular map for RG211877

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANKS1B
• Synonyms: AIDA; AIDA-1; ANKS2; cajalin-2; EB-1; EB1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211877 representing NM_181670
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGGCAATGCCACCTCTCAGCCCAGGACTACCGCTATTACCCCGTGGACGGCTACTCCCTGCTTA
  AACGCTTCCCTCTTCATCCTCTTACAGGACCCAGATGCCCTGTCCAAACAGTGGGACAATGGTTGGAAAG
  CATTGGGCTACCTCAGTACGAGAACCACCTGATGGCTAATGGATTTGACAATGTGCAGTTTATGGGAAGC
  AATGTTATGGAAGATCAGGATTTGTTGGAAATTGGAATCCTTAATTCTGGGCACAGACAAAGAATTCTAC
  AGGCAATCCAGCTCCTTCCAAAGATGAGACCCATTGGGCATGATGGCTACCATCCCACCTCTGTAGCTGA
  GTGGCTGGATTCCATTGAACTGGGCGACTACACCAAAGCCTTTCTAATTAATGGCTACACTTCGATGGAC
  CTGTTGAAAAAAATCTGGGAGGTTGAACTTATTAATGTTTTAAAAATCAATTTGATTGGCCACAGGAAAC
  GTATTTTGGCATCTCTGGGAGACAGGCTGCACGACGATCCCCCACAGAAGCCCCCTCGGTCCATCACCCT
  CAGGGAACCCAGTGGTAATCACACTCCTCCTCAGTTGTCTCCATCACTTAGCCAAAGCACTTACACCACT
  GGTGGCTCCCTAGACGTTCCTCACATTATCATGCAGGGCGATGCAAGGAGGAGAAGAAATGAAAACTACT
  TTGATGATATTCCCCGATCAAAACTGGAGAGGCAGATGGCTCAGTCGTCTGTCTGTGAAATATGGACGAA
  TCAGAACGCAGGATTTCCTTTCTCAGCGATCCATCAGGTTCATAATACAGGAGACTGGGGAGAACCTTCC
  ATTACCTTGCGACCTCCGAATGAAGCCACAGCCTCTACCCCGGTACAGTACTGGCAGCATCACCCAGAAA
  AGCTTATCTTCCAGTCGTGTGATTACAAAGCTTTTTATTTAGGTTCTATGCTGATAAAAGAGCTTAGGGG
  GACAGAATCAACCCAAGATGCTTGTGCAAAAATGCGGGCTAACTGTCAGAAGTCTACAGAGCAAATGAAG
  AAGGTCCCTACTATTATTCTTTCTGTCTCATATAAAGGAGTCAAATTTATTGATGCAACAAATAAGAACA
  TAATTGCTGAGCATGAAATTCGTAATATCTCCTGTGCTGCCCAGGACCCAGAAGACCTCTCAACATTTGC
  CTATATCACAAAAGATTTGAAGTCTAATCACCACTACTGTCATGTGTTTACTGCCTTTGATGTGAATTTA
  GCCTATGAAATCATCCTAACCCTGGGACAGGCATTCGAAGTCGCTTACCAGCTAGCACTACAAGCAAGAA
  AAGGGGGACACTCCTCCACACTTCCAGAAAGCTTTGAAAACAAACCCTCCAAACCCATCCCCAAGCCCCG
  CGTTAGCATTCGCAAGTCCGTGCAGATCGACCCATCTGAGCAAAAGACTCTGGCCAATCTACCGTGGATT
  GTGGAGCCGGGCCAAGAAGCCAAGAGGGGCATTAATACCAAGTATGAAACCACGATTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211877 representing NM_181670
  Red=Cloning site Green=Tags(s)
  
  MMWQCHLSAQDYRYYPVDGYSLLKRFPLHPLTGPRCPVQTVGQWLESIGLPQYENHLMANGFDNVQFMGS
  NVMEDQDLLEIGILNSGHRQRILQAIQLLPKMRPIGHDGYHPTSVAEWLDSIELGDYTKAFLINGYTSMD
  LLKKIWEVELINVLKINLIGHRKRILASLGDRLHDDPPQKPPRSITLREPSGNHTPPQLSPSLSQSTYTT
  GGSLDVPHIIMQGDARRRRNENYFDDIPRSKLERQMAQSSVCEIWTNQNAGFPFSAIHQVHNTGDWGEPS
  ITLRPPNEATASTPVQYWQHHPEKLIFQSCDYKAFYLGSMLIKELRGTESTQDACAKMRANCQKSTEQMK
  KVPTIILSVSYKGVKFIDATNKNIIAEHEIRNISCAAQDPEDLSTFAYITKDLKSNHHYCHVFTAFDVNL
  AYEIILTLGQAFEVAYQLALQARKGGHSSTLPESFENKPSKPIPKPRVSIRKSVQIDPSEQKTLANLPWI
  VEPGQEAKRGINTKYETTIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181670
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181670.4
• RefSeq Size: 3287 bp
• RefSeq ORF: 1533 bp
• Locus ID: 56899
• UniProt ID: Q7Z6G8
• Cytogenetics: 12q23.1
• Gene Summary: This gene encodes a multi-domain protein that is predominantly expressed in brain and testis. This protein interacts with amyloid beta protein precursor (AbetaPP) and may have a role in normal brain development, and in the pathogenesis of Alzheimer's disease. Expression of this gene has been shown to be elevated in patients with pre-B cell acute lymphocytic leukemia associated with t(1;19) translocation. Alternatively spliced transcript variants encoding different isoforms (some with different subcellular localization, PMID:15004329) have been described for this gene. [provided by RefSeq, Aug 2011]