HOXB3 (NM_002146) Human Tagged ORF Clone

CAT#: RG211780

HOXB3 (tGFP-tagged) - Human homeobox B3 (HOXB3)

Product Images

Circular map for RG211780

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HOXB3
• Synonyms: Hox-2.7; HOX2; HOX2G
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211780 representing NM_002146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAAGCCACCTACTACGACAACGCCGCGGCTGCTCTCTTCGGAGGCTATTCCTCGTACCCTGGCA
  GCAATGGCTTCGGCTTCGATGTCCCCCCCCAACCCCCATTTCAGGCCGCCACGCACCTGGAGGGCGACTA
  CCAGCGCTCAGCTTGCTCGCTGCAGTCCCTGGGCAACGCTGCCCCACATGCCAAGAGCAAGGAGCTCAAC
  GGCAGCTGCATGAGGCCGGGTCTGGCCCCCGAGCCCCTGTCGGCCCCGCCTGGCTCACCCCCGCCCAGTG
  CCGCACCTACCAGTGCCACTAGCAACAGCAGTAATGGGGGCGGGCCCAGCAAAAGTGGTCCCCCAAAGTG
  CGGTCCCGGCACCAACTCCACCCTCACCAAACAGATATTCCCCTGGATGAAAGAGTCGAGGCAAACGTCC
  AAGCTGAAAAACAACTCCCCCGGCACAGCAGAGGGCTGTGGTGGCGGCGGCGGTGGCGGCGGCGGCGGAG
  GCAGTGGTGGCAGCGGGGGCGGTGGCGGCGGCGGCGGGGGAGGGGACAAGAGCCCCCCGGGGTCGGCGGC
  GTCCAAGCGGGCGCGGACGGCGTACACGAGCGCGCAGCTGGTGGAGCTGGAGAAGGAGTTCCATTTTAAC
  CGCTACCTGTGCCGGCCTCGCCGTGTAGAGATGGCCAACCTGCTGAACCTCAGCGAGCGGCAGATCAAGA
  TCTGGTTCCAGAACCGGCGCATGAAGTACAAGAAGGACCAGAAGGCCAAGGGATTGGCCTCGTCGTCGGG
  GGGCCCATCTCCAGCCGGCAGCCCCCCGCAGCCCATGCAGTCCACGGCCGGCTTCATGAACGCCTTACAC
  TCCATGACCCCCAGCTACGAGAGCCCGTCCCCACCCGCCTTCGGTAAAGCCCACCAGAATGCCTACGCGC
  TGCCCTCCAACTACCAGCCCCCTCTCAAAGGCTGCGGCGCCCCGCAGAAGTACCCTCCGACCCCGGCGCC
  CGAGTATGAGCCGCACGTCCTCCAAGCCAACGGGGGCGCCTACGGGACGCCCACCATGCAGGGCAGTCCG
  GTGTACGTGGGCGGGGGCGGCTACGCGGATCCGCTGCCGCCCCCTGCCGGCCCCTCCCTCTATGGCCTCA
  ACCACCTTTCCCATCACCCTTCCGGGAACCTGGACTACAACGGGGCGCCCCCTATGGCGCCCAGCCAGCA
  CCACGGACCCTGCGAACCCCACCCCACCTACACAGACCTCTCCTCTCACCACGCGCCTCCTCCTCAGGGT
  AGAATCCAAGAAGCGCCCAAATTAACACACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211780 representing NM_002146
  Red=Cloning site Green=Tags(s)
  
  MQKATYYDNAAAALFGGYSSYPGSNGFGFDVPPQPPFQAATHLEGDYQRSACSLQSLGNAAPHAKSKELN
  GSCMRPGLAPEPLSAPPGSPPPSAAPTSATSNSSNGGGPSKSGPPKCGPGTNSTLTKQIFPWMKESRQTS
  KLKNNSPGTAEGCGGGGGGGGGGGSGGSGGGGGGGGGGDKSPPGSAASKRARTAYTSAQLVELEKEFHFN
  RYLCRPRRVEMANLLNLSERQIKIWFQNRRMKYKKDQKAKGLASSSGGPSPAGSPPQPMQSTAGFMNALH
  SMTPSYESPSPPAFGKAHQNAYALPSNYQPPLKGCGAPQKYPPTPAPEYEPHVLQANGGAYGTPTMQGSP
  VYVGGGGYADPLPPPAGPSLYGLNHLSHHPSGNLDYNGAPPMAPSQHHGPCEPHPTYTDLSSHHAPPPQG
  RIQEAPKLTHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002146
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002146.4, NP_002137.4
• RefSeq Size: 3627 bp
• RefSeq ORF: 1296 bp
• Locus ID: 3213
• UniProt ID: P14651
• Cytogenetics: 17q21.32
• Gene Summary: This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Increased expression of this gene is associated with a distinct biologic subset of acute myeloid leukemia (AML). [provided by RefSeq, Jul 2008]