HDAC4 (NM_006037) Human Tagged ORF Clone

CAT#: RG211495

HDAC4 (tGFP-tagged) - Human histone deacetylase 4 (HDAC4)

Product Images

Circular map for RG211495

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HDAC4
• Synonyms: AHO3; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDACA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211495 representing NM_006037
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCCAAAGCCATCCAGATGGACTTTCTGGCCGAGACCAGCCAGTGGAGCTGCTGAATCCTGCCC
  GCGTGAACCACATGCCCAGCACGGTGGATGTGGCCACGGCGCTGCCTCTGCAAGTGGCCCCCTCGGCAGT
  GCCCATGGACCTGCGCCTGGACCACCAGTTCTCACTGCCTGTGGCAGAGCCGGCCCTGCGGGAGCAGCAG
  CTGCAGCAGGAGCTCCTGGCGCTCAAGCAGAAGCAGCAGATCCAGAGGCAGATCCTCATCGCTGAGTTCC
  AGAGGCAGCACGAGCAGCTCTCCCGGCAGCACGAGGCGCAGCTCCACGAGCACATCAAGCAACAACAGGA
  GATGCTGGCCATGAAGCACCAGCAGGAGCTGCTGGAACACCAGCGGAAGCTGGAGAGGCACCGCCAGGAG
  CAGGAGCTGGAGAAGCAGCACCGGGAGCAGAAGCTGCAGCAGCTCAAGAACAAGGAGAAGGGCAAAGAGA
  GTGCCGTGGCCAGCACAGAAGTGAAGATGAAGTTACAAGAATTTGTCCTCAATAAAAAGAAGGCGCTGGC
  CCACCGGAATCTGAACCACTGCATTTCCAGCGACCCTCGCTACTGGTACGGGAAAACGCAGCACAGTTCC
  CTTGACCAGAGTTCTCCACCCCAGAGCGGAGTGTCGACCTCCTATAACCACCCGGTCCTGGGAATGTACG
  ACGCCAAAGATGACTTCCCTCTTAGGAAAACAGCTTCTGAACCGAATCTGAAATTACGGTCCAGGCTAAA
  GCAGAAAGTGGCCGAAAGACGGAGCAGCCCCCTGTTACGCAGGAAAGACGGGCCAGTGGTCACTGCTCTA
  AAAAAGCGTCCGTTGGATGTCACAGACTCCGCGTGCAGCAGCGCCCCAGGCTCCGGACCCAGCTCACCCA
  ACAACAGCTCCGGGAGCGTCAGCGCGGAGAACGGTATCGCGCCCGCCGTCCCCAGCATCCCGGCGGAGAC
  GAGTTTGGCGCACAGACTTGTGGCACGAGAAGGCTCGGCCGCTCCACTTCCCCTCTACACATCGCCATCC
  TTGCCCAACATCACGCTGGGCCTGCCTGCCACCGGCCCCTCTGCGGGCACGGCGGGCCAGCAGGACGCCG
  AGAGACTCACCCTTCCCGCCCTCCAGCAGAGGCTCTCCCTTTTCCCCGGCACCCACCTCACTCCCTACCT
  GAGCACCTCGCCCTTGGAGCGGGACGGAGGGGCAGCGCACAGCCCTCTTCTGCAGCACATGGTCTTACTG
  GAGCAGCCGCCGGCACAAGCACCCCTCGTCACAGGCCTGGGAGCACTGCCCCTCCACGCACAGTCCTTGG
  TTGGTGCAGACCGGGTGTCCCCCTCCATCCACAAGCTGCGGCAGCACCGCCCACTGGGGCGGACCCAGTC
  GGCCCCGCTGCCCCAGAACGCCCAGGCTCTGCAGCACCTGGTCATCCAGCAGCAGCATCAGCAGTTTCTG
  GAGAAACACAAGCAGCAGTTCCAGCAGCAGCAACTGCAGATGAACAAGATCATCCCCAAGCCAAGCGAGC
  CAGCCCGGCAGCCGGAGAGCCACCCGGAGGAGACGGAGGAGGAGCTCCGTGAGCACCAGGCTCTGCTGGA
  CGAGCCCTACCTGGACCGGCTGCCGGGGCAGAAGGAGGCGCACGCACAGGCCGGCGTGCAGGTGAAGCAG
  GAGCCCATTGAGAGCGATGAGGAAGAGGCAGAGCCCCCACGGGAGGTGGAGCCGGGCCAGCGCCAGCCCA
  GTGAGCAGGAGCTGCTCTTCAGACAGCAAGCCCTCCTGCTGGAGCAGCAGCGGATCCACCAGCTGAGGAA
  CTACCAGGCGTCCATGGAGGCCGCCGGCATCCCCGTGTCCTTCGGCGGCCACAGGCCTCTGTCCCGGGCG
  CAGTCCTCACCCGCGTCTGCCACCTTCCCCGTGTCTGTGCAGGAGCCCCCCACCAAGCCGAGGTTCACGA
  CAGGCCTCGTGTATGACACGCTGATGCTGAAGCACCAGTGCACCTGCGGGAGTAGCAGCAGCCACCCCGA
  GCACGCCGGGAGGATCCAGAGCATCTGGTCCCGCCTGCAGGAGACGGGCCTCCGGGGCAAATGCGAGTGC
  ATCCGCGGACGCAAGGCCACCCTGGAGGAGCTACAGACGGTGCACTCGGAAGCCCACACCCTCCTGTATG
  GCACGAACCCCCTCAACCGGCAGAAACTGGACAGTAAGAAACTTCTAGGCTCGCTCGCCTCCGTGTTCGT
  CCGGCTCCCTTGCGGTGGTGTTGGGGTGGACAGTGACACCATATGGAACGAGGTGCACTCGGCGGGGGCA
  GCCCGCCTGGCTGTGGGCTGCGTGGTAGAGCTGGTCTTCAAGGTGGCCACAGGGGAGCTGAAGAATGGCT
  TTGCTGTGGTCCGCCCCCCTGGACACCATGCGGAGGAGAGCACGCCCATGGGCTTTTGCTACTTCAACTC
  CGTGGCCGTGGCAGCCAAGCTTCTGCAGCAGAGGTTGAGCGTGAGCAAGATCCTCATCGTGGACTGGGAC
  GTGCACCATGGAAACGGGACCCAGCAGGCTTTCTACAGCGACCCCAGCGTCCTGTACATGTCCCTCCACC
  GCTACGACGATGGGAACTTCTTCCCAGGCAGCGGGGCTCCTGATGAGGTGGGCACAGGGCCCGGCGTGGG
  TTTCAACGTCAACATGGCTTTCACCGGCGGCCTGGACCCCCCCATGGGAGACGCTGAGTACTTGGCGGCC
  TTCAGAACGGTGGTCATGCCGATCGCCAGCGAGTTTGCCCCGGATGTGGTGCTGGTGTCATCAGGCTTCG
  ATGCCGTGGAGGGCCACCCCACCCCTCTTGGGGGCTACAACCTCTCCGCCAGATGCTTCGGGTACCTGAC
  GAAGCAGCTGATGGGCCTGGCTGGCGGCCGGATTGTCCTGGCCCTCGAGGGAGGCCACGACCTGACCGCC
  ATTTGCGACGCCTCGGAAGCATGTGTTTCTGCCTTGCTGGGAAACGAGCTTGATCCTCTCCCAGAAAAGG
  TTTTACAGCAAAGACCCAATGCAAACGCTGTCCGTTCCATGGAGAAAGTCATGGAGATCCACAGCAAGTA
  CTGGCGCTGCCTGCAGCGCACAACCTCCACAGCGGGGCGTTCTCTGATCGAGGCTCAGACTTGCGAGAAC
  GAAGAAGCCGAGACGGTCACCGCCATGGCCTCGCTGTCCGTGGGCGTGAAGCCCGCCGAAAAGAGACCAG
  ATGAGGAGCCCATGGAAGAGGAGCCGCCCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211495 representing NM_006037
  Red=Cloning site Green=Tags(s)
  
  MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQ
  LQQELLALKQKQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQE
  QELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSS
  LDQSSPPQSGVSTSYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVVTAL
  KKRPLDVTDSACSSAPGSGPSSPNNSSGSVSAENGIAPAVPSIPAETSLAHRLVAREGSAAPLPLYTSPS
  LPNITLGLPATGPSAGTAGQQDAERLTLPALQQRLSLFPGTHLTPYLSTSPLERDGGAAHSPLLQHMVLL
  EQPPAQAPLVTGLGALPLHAQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFL
  EKHKQQFQQQQLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEAHAQAGVQVKQ
  EPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGGHRPLSRA
  QSSPASATFPVSVQEPPTKPRFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCEC
  IRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGA
  ARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWD
  VHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDPPMGDAEYLAA
  FRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQLMGLAGGRIVLALEGGHDLTA
  ICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCEN
  EEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006037
• ORF Size: 3252 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006037.4
• RefSeq Size: 8459 bp
• RefSeq ORF: 3255 bp
• Locus ID: 9759
• UniProt ID: P56524
• Cytogenetics: 2q37.3
• Domains: Hist_deacetyl
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]