DNA polymerase mu (POLM) (NM_013284) Human Tagged ORF Clone

CAT#: RG211447

POLM (tGFP-tagged) - Human polymerase (DNA directed), mu (POLM)

Product Images

Circular map for RG211447

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DNA polymerase mu
• Synonyms: Pol Mu; Tdt-N
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211447 representing NM_013284
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCCCAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCT
  CGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAGCCTCGCATGGGTCGCAGCCGCCGGGCCTTCCT
  CACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTGTG
  ATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTT
  GCACCCCCCCAGCTCTGCTGGACATAAGCTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGT
  GGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCATGGATGCCTGCC
  TATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAGATACTGG
  CCGAGGCAGCAGGCTTTGAAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCGGTGCTCAA
  GGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCCCCACTTTGGAGAACACTCCTCT
  AGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGCTCAGAGAGGT
  ACCAGACCATGAAGCTCTTCACCCAGATCTTCGGGGTCGGTGTGAAGACTGCTGACCGGTGGTACCGGGA
  AGGACTGCGAACCTTAGATGACCTCCGAGAGCAGCCCCAGAAACTAACCCAACAGCAGAAAGCGGGGCTC
  CAGCACCACCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCAGCAGGTGGTGGAGG
  AAGCTGTGGGGCAGGCCCTGCCTGGGGCCACCGTCACGCTGACCGGCGGCTTCCGCAGGGGGAAGTTGCA
  GGGCCATGACGTGGACTTCCTCATCACCCACCCCAAGGAGGGTCAGGAGGCGGGGCTGCTGCCTAGAGTG
  ATGTGCCGCCTGCAGGACCAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCTGTGAGTCCCCTA
  CCCGCCTGGCCCAACAGAGCCACATGGACGCTTTTGAGAGAAGTTTCTGCATTTTCCGCCTACCACAACC
  TCCAGGGGCTGCTGTGGGGGGATCCACGAGGCCCTGCCCATCCTGGAAGGCCGTGAGAGTGGACTTGGTA
  GTTGCACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGCTCCAAGCTTTTCCAGCGGGAGC
  TGCGCCGCTTCAGCCGGAAGGAGAAGGGCCTGTGGCTGAACAGCCATGGGCTGTTTGACCCGGAGCAGAA
  GACATTTTTCCAAGCGGCTTCAGAGGAAGACATCTTCAGACACCTGGGCCTTGAGTACCTTCCTCCAGAG
  CAGAGAAACGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211447 representing NM_013284
  Red=Cloning site Green=Tags(s)
  
  MLPKRRRARVGSPSGDAASSTPPSTRFPGVAIYLVEPRMGRSRRAFLTGLARSKGFRVLDACSSEATHVV
  MEETSAEEAVSWQERRMAAAPPGCTPPALLDISWLTESLGAGQPVPVECRHRLEVAGPRKGPLSPAWMPA
  YACQRPTPLTHHNTGLSEALEILAEAAGFEGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLPHFGEHSS
  RVVQELLEHGVCEEVERVRRSERYQTMKLFTQIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQQKAGL
  QHHQDLSTPVLRSDVDALQQVVEEAVGQALPGATVTLTGGFRRGKLQGHDVDFLITHPKEGQEAGLLPRV
  MCRLQDQGLILYHQHQHSCCESPTRLAQQSHMDAFERSFCIFRLPQPPGAAVGGSTRPCPSWKAVRVDLV
  VAPVSQFPFALLGWTGSKLFQRELRRFSRKEKGLWLNSHGLFDPEQKTFFQAASEEDIFRHLGLEYLPPE
  QRNA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013284
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013284.4
• RefSeq Size: 2589 bp
• RefSeq ORF: 1485 bp
• Locus ID: 27434
• UniProt ID: Q9NP87
• Cytogenetics: 7p13
• Domains: POLXc
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• Gene Summary: Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.[UniProtKB/Swiss-Prot Function]