GBA3 (NM_020973) Human Tagged ORF Clone

CAT#: RG211035

GBA3 (tGFP-tagged) - Human glucosidase, beta, acid 3 (cytosolic) (GBA3), transcript variant 1

Product Images

Circular map for RG211035

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GBA3
• Synonyms: CBG; CBGL1; GLUC; KLRP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211035 representing NM_020973
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCCCTGCAGGATTTGGATGGGCGGCAGCCACTGCAGCTTATCAAGTAGAAGGAGGCTGGGATG
  CAGATGGAAAAGGCCCTTGTGTCTGGGACACATTTACTCATCAGGGAGGAGAGAGAGTTTTCAAGAACCA
  GACTGGCGATGTAGCTTGTGGCAGCTACACTCTGTGGGAGGAAGATTTGAAATGTATCAAACAGCTTGGA
  TTGACTCATTACCGCTTCTCTCTTTCCTGGTCACGTCTGTTACCTGATGGGACGACAGGTTTCATCAACC
  AGAAAGGAATTGATTATTACAACAAGATCATCGATGATTTGTTAAAAAATGGGGTTACTCCCATTGTGAC
  CCTCTACCACTTTGATTTGCCTCAGACTTTAGAAGACCAAGGAGGTTGGTTGTCAGAGGCAATCATTGAA
  TCCTTTGACAAATATGCTCAGTTTTGCTTCAGTACCTTTGGGGATCGTGTCAAGCAGTGGATCACCATAA
  ATGAAGCTAATGTTCTTTCTGTGATGTCATATGACTTAGGTATGTTTCCTCCGGGTATCCCTCACTTTGG
  GACTGGAGGTTATCAGGCAGCTCATAATTTGATTAAGGCTCATGCCAGATCCTGGCACAGCTATGATTCC
  TTATTTCGAAAAAAGCAGAAAGGTATGGTGTCTCTATCACTTTTTGCGGTCTGGTTGGAACCAGCAGATC
  CCAACTCAGTGTCTGACCAGGAAGCTGCTAAAAGAGCCATCACTTTCCATCTGGATTTATTTGCTAAACC
  CATATTCATCGATGGTGATTATCCTGAAGTTGTCAAGTCTCAGATTGCCTCCATGAGTCAAAAGCAAGGC
  TATCCATCATCGAGGCTTCCAGAATTCACTGAAGAAGAGAAGAAAATGATCAAAGGCACTGCTGATTTTT
  TTGCTGTGCAATATTATACAACTCGCTTAATCAAGTACCAGGAGAACAAGAAAGGAGAACTAGGTATTCT
  CCAGGATGCGGAAATTGAATTTTTTCCAGATCCATCTTGGAAAAATGTGGATTGGATCTACGTGGTACCA
  TGGGGAGTATGTAAACTACTGAAATATATTAAGGATACATATAATAACCCTGTAATTTACATCACTGAGA
  ATGGGTTTCCCCAGAGTGACCCAGCGCCTCTTGATGACACTCAACGCTGGGAGTATTTCAGACAAACATT
  TCAGGAACTGTTCAAAGCTATCCAACTTGATAAAGTCAATCTTCAAGTATATTGTGCATGGTCTCTTCTG
  GATAACTTTGAGTGGAACCAGGGATACAGCAGCCGGTTTGGTCTCTTCCACGTTGATTTTGAAGACCCAG
  CTAGACCCCGAGTCCCTTACACATCGGCCAAGGAATATGCCAAGATCATCCGAAACAATGGCCTTGAAGC
  ACATCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211035 representing NM_020973
  Red=Cloning site Green=Tags(s)
  
  MAFPAGFGWAAATAAYQVEGGWDADGKGPCVWDTFTHQGGERVFKNQTGDVACGSYTLWEEDLKCIKQLG
  LTHYRFSLSWSRLLPDGTTGFINQKGIDYYNKIIDDLLKNGVTPIVTLYHFDLPQTLEDQGGWLSEAIIE
  SFDKYAQFCFSTFGDRVKQWITINEANVLSVMSYDLGMFPPGIPHFGTGGYQAAHNLIKAHARSWHSYDS
  LFRKKQKGMVSLSLFAVWLEPADPNSVSDQEAAKRAITFHLDLFAKPIFIDGDYPEVVKSQIASMSQKQG
  YPSSRLPEFTEEEKKMIKGTADFFAVQYYTTRLIKYQENKKGELGILQDAEIEFFPDPSWKNVDWIYVVP
  WGVCKLLKYIKDTYNNPVIYITENGFPQSDPAPLDDTQRWEYFRQTFQELFKAIQLDKVNLQVYCAWSLL
  DNFEWNQGYSSRFGLFHVDFEDPARPRVPYTSAKEYAKIIRNNGLEAHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020973
• ORF Size: 1407 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020973.4
• RefSeq Size: 2125 bp
• RefSeq ORF: 1410 bp
• Locus ID: 57733
• UniProt ID: Q9H227
• Cytogenetics: 4p15.2
• Protein Pathways: Cyanoamino acid metabolism, Starch and sucrose metabolism
• Gene Summary: The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013]