EXOSC5 (NM_020158) Human Tagged ORF Clone

CAT#: RG210665

EXOSC5 (tGFP-tagged) - Human exosome component 5 (EXOSC5)

Product Images

Circular map for RG210665

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EXOSC5
• Synonyms: hRrp46p; p12B; RRP41B; RRP46; Rrp46p
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210665 representing NM_020158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGAGATGCATACTGACGCCAAAATCCGTGCTGAAAATGGAACAGGGTCCAGCCCTCGGGGTC
  CTGGCTGCAGCCTCCGGCACTTTGCCTGCGAACAGAACCTGCTGTCGCGGCCAGATGGCTCTGCTTCCTT
  CCTGCAAGGTGACACCTCTGTCCTGGCGGGTGTGTACGGGCCGGCCGAGGTGAAGGTCAGCAAAGAGATT
  TTCAACAAGGCCACACTCGAAGTGATCCTGAGGCCGAAGATTGGGCTGCCTGGTGTTGCAGAGAAGAGCC
  GGGAGCGGCTGATCAGGAACACGTGCGAGGCGGTGGTGCTGGGCACGTTGCACCCCCGCACCTCCATCAC
  CGTGGTGCTGCAGGTTGTCAGCGATGCCGGCTCTCTCCTGGCCTGTTGTCTGAATGCCGCCTGCATGGCA
  TTGGTGGATGCAGGTGTGCCCATGCGGGCTCTCTTCTGTGGGGTCGCCTGCGCCCTGGACTCTGATGGGA
  CCCTCGTGCTGGATCCTACATCCAAGCAAGAAAAGGAGGCCCGGGCAGTCCTGACCTTTGCCCTGGACAG
  CGTGGAACGGAAGCTGCTGATGTCCAGCACCAAGGGGCTCTACTCAGACACTGAGCTCCAGCAGTGCCTG
  GCTGCGGCCCAGGCCGCTTCGCAACACGTCTTCCGTTTCTACCGGGAATCGCTGCAGAGGCGTTACTCCA
  AGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210665 representing NM_020158
  Red=Cloning site Green=Tags(s)
  
  MEEEMHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQGDTSVLAGVYGPAEVKVSKEI
  FNKATLEVILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMA
  LVDAGVPMRALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCL
  AAAQAASQHVFRFYRESLQRRYSKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020158
• ORF Size: 705 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020158.3, NP_064543.3
• RefSeq Size: 1006 bp
• RefSeq ORF: 708 bp
• Locus ID: 56915
• UniProt ID: Q9NQT4
• Cytogenetics: 19q13.2
• Protein Pathways: RNA degradation
• Gene Summary: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.[UniProtKB/Swiss-Prot Function]