KCNN1 (NM_002248) Human Tagged ORF Clone

CAT#: RG210434

KCNN1 (tGFP-tagged) - Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1 (KCNN1)

Product Images

Circular map for RG210434

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNN1
• Synonyms: hSK1; KCa2.1; SK1; SKCA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210434 representing NM_002248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCCACAGCTACAATGGCAGCGTGGGGCGGCCGCTGGGCAGCGGGCCGGGCGCCCTGGGACGAG
  ACCCTCCGGACCCTGAGGCCGGCCACCCCCCACAACCCCCGCACAGCCCGGGCCTCCAGGTGGTAGTGGC
  CAAGAGTGAGCCAGCCCGGCCCTCACCCGGCAGCCCCCGGGGGCAGCCCCAGGACCAGGACGATGACGAG
  GATGATGAGGAAGATGAGGCCGGCAGGCAGAGAGCCTCGGGGAAACCCTCAAATGTGGGCCACCGCCTGG
  GCCACCGGCGGGCGCTCTTCGAGAAGCGGAAGCGCCTCAGCGACTATGCCCTCATTTTCGGCATGTTTGG
  CATCGTCGTCATGGTGACGGAGACCGAGCTGTCCTGGGGGGTGTACACCAAGGAGTCTCTGTACTCATTC
  GCACTCAAATGCCTCATCAGCCTCTCCACGGCCATCCTGCTGGGTCTCGTTGTCCTCTACCATGCCCGGG
  AGATCCAGCTGTTCATGGTGGACAACGGGGCTGATGACTGGCGCATCGCCATGACCTGCGAGCGCGTGTT
  CCTCATCTCGCTAGAGCTGGCAGTGTGCGCCATTCACCCGGTGCCCGGCCACTACCGCTTCACGTGGACG
  GCGCGGCTGGCCTTCACGTACGCGCCCTCGGTGGCCGAGGCCGACGTGGACGTGCTGCTGTCCATCCCCA
  TGTTCCTGCGCCTCTACCTGCTGGGCCGGGTGATGCTACTGCACAGCAAAATCTTCACGGACGCCTCGAG
  CCGCAGCATCGGGGCCCTCAACAAGATCACCTTCAACACGCGCTTCGTCATGAAGACACTCATGACCATC
  TGCCCCGGCACCGTGCTGCTGGTCTTCAGCATCTCCTCCTGGATCATCGCAGCCTGGACCGTGCGCGTCT
  GCGAGAGGTACCACGACAAGCAGGAAGTGACCAGCAACTTCCTGGGGGCCATGTGGCTGATTTCCATCAC
  CTTCCTCTCCATTGGCTACGGCGACATGGTGCCCCACACCTACTGCGGGAAGGGTGTGTGCCTGCTCACT
  GGCATCATGGGAGCTGGCTGTACCGCGCTCGTGGTGGCTGTGGTGGCTCGGAAGCTGGAGCTCACCAAGG
  CTGAGAAGCACGTGCACAACTTCATGATGGACACTCAGCTCACCAAGCGGGTAAAAAACGCCGCTGCTAA
  CGTTCTCAGGGAGACGTGGCTCATCTACAAACATACCAGGCTGGTGAAGAAGCCAGACCAAGCCCGGGTT
  CGGAAACACCAGCGTAAGTTCCTCCAAGCCATCCATCAGGCTCAGAAGCTCCGGAGTGTGAAGATCGAGC
  AAGGGAAGCTGAACGACCAGGCTAACACGCTTACCGACCTAGCCAAGACCCAGACCGTCATGTACGACCT
  TGTATCGGAGCTGCACGCTCAGCACGAGGAGCTGGAGGCCCGCCTGGCCACCCTGGAAAGCCGCTTGGAT
  GCGCTGGGTGCCTCTCTACAGGCCCTGCCTGGCCTCATCGCCCAAGCCATACGCCCACCCCCGCCTCCCC
  TGCCTCCCAGGCCCGGCCCCGGCCCCCAAGACCAGGCAGCCCGGAGCTCCCCCTGCCGGTGGACGCCCGT
  GGCCCCCTCGGACTGCGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210434 representing NM_002248
  Red=Cloning site Green=Tags(s)
  
  MNSHSYNGSVGRPLGSGPGALGRDPPDPEAGHPPQPPHSPGLQVVVAKSEPARPSPGSPRGQPQDQDDDE
  DDEEDEAGRQRASGKPSNVGHRLGHRRALFEKRKRLSDYALIFGMFGIVVMVTETELSWGVYTKESLYSF
  ALKCLISLSTAILLGLVVLYHAREIQLFMVDNGADDWRIAMTCERVFLISLELAVCAIHPVPGHYRFTWT
  ARLAFTYAPSVAEADVDVLLSIPMFLRLYLLGRVMLLHSKIFTDASSRSIGALNKITFNTRFVMKTLMTI
  CPGTVLLVFSISSWIIAAWTVRVCERYHDKQEVTSNFLGAMWLISITFLSIGYGDMVPHTYCGKGVCLLT
  GIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKHTRLVKKPDQARV
  RKHQRKFLQAIHQAQKLRSVKIEQGKLNDQANTLTDLAKTQTVMYDLVSELHAQHEELEARLATLESRLD
  ALGASLQALPGLIAQAIRPPPPPLPPRPGPGPQDQAARSSPCRWTPVAPSDCG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002248
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002248.4
• RefSeq Size: 2657 bp
• RefSeq ORF: 1632 bp
• Locus ID: 3780
• UniProt ID: Q92952
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. This gene is a member of the KCNN family of potassium channel genes. [provided by RefSeq, Jul 2008]