PAX4 (NM_006193) Human Tagged ORF Clone

CAT#: RG210370

PAX4 (tGFP-tagged) - Human paired box 4 (PAX4)

Product Images

Circular map for RG210370

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PAX4
• Synonyms: KPD; MODY9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210370 representing NM_006193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCAGCTTGGGGGGCTCTTTGTGAATGGCCGGCCCCTGCCTCTGGATACCCGGCAGCAGATTGTGC
  GGCTAGCAGTCAGTGGAATGCGGCCCTGTGACATCTCACGGATCCTTAAGGTATCTAATGGCTGTGTGAG
  CAAGATCCTAGGGCGTTACTACCGCACAGGTGTCTTGGAGCCAAAGGGCATTGGGGGAAGCAAGCCACGG
  CTGGCTACACCCCCTGTGGTGGCTCGAATTGCCCAGCTGAAGGGTGAGTGTCCAGCCCTCTTTGCCTGGG
  AAATCCAACGCCAGCTTTGTGCTGAAGGGCTTTGCACCCAGGACAAGACTCCCAGTGTCTCCTCCATCAA
  CCGAGTCCTGCGGGCATTACAGGAGGACCAGGGACTACCGTGCACACGGCTCAGGTCACCAGCTGTTTTG
  GCTCCAGCTGTCCTCACTCCCCATAGTGGCTCTGAGACTCCCCGGGGTACCCACCCAGGGACCGGCCACC
  GGAATCGGACTATCTTCTCCCCAAGCCAGGCAGAGGCACTGGAGAAAGAGTTCCAGCGTGGGCAGTATCC
  TGATTCAGTGGCCCGTGGAAAGCTGGCTACTGCCACCTCTCTGCCTGAGGACACGGTGAGGGTCTGGTTT
  TCCAACAGAAGAGCCAAATGGCGTCGGCAAGAGAAGCTCAAGTGGGAAATGCAGCTGCCAGGTGCTTCCC
  AGGGGCTGACTGTACCAAGGGTTGCCCCAGGAATCATCTCTGCACAGCAGTCCCCTGGCAGTGTGCCCAC
  AGCAGCCCTGCCTGCCCTGGAACCACTGGGTCCCTCCTGCTATCAGCTGTGCTGGGCAACAGCACCAGAA
  AGGTGTCTGAGTGACACCCCACCTAAAGCCTGTCTCAAGCCCTGCTGGGGCCACTTGCCCCCACAGCCGA
  ATTCCCTGGACTCAGGACTGCTTTGCCTTCCTTGCCCTTCCTCCCACTGTCCCCTGGCCAGTCTTAGTGG
  CTCTCAGGCCCTGCTCTGGCCTGGCTGCCCACTACTGTATGGCTTGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210370 representing NM_006193
  Red=Cloning site Green=Tags(s)
  
  MNQLGGLFVNGRPLPLDTRQQIVRLAVSGMRPCDISRILKVSNGCVSKILGRYYRTGVLEPKGIGGSKPR
  LATPPVVARIAQLKGECPALFAWEIQRQLCAEGLCTQDKTPSVSSINRVLRALQEDQGLPCTRLRSPAVL
  APAVLTPHSGSETPRGTHPGTGHRNRTIFSPSQAEALEKEFQRGQYPDSVARGKLATATSLPEDTVRVWF
  SNRRAKWRRQEKLKWEMQLPGASQGLTVPRVAPGIISAQQSPGSVPTAALPALEPLGPSCYQLCWATAPE
  RCLSDTPPKACLKPCWGHLPPQPNSLDSGLLCLPCPSSHCPLASLSGSQALLWPGCPLLYGLE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006193
• ORF Size: 1029 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006193.1, NP_006184.1
• RefSeq Size: 2020 bp
• RefSeq ORF: 1032 bp
• Locus ID: 5078
• UniProt ID: O43316
• Cytogenetics: 7q32.1
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells. [provided by RefSeq, Jul 2008]