C4orf17 (NM_032149) Human Tagged ORF Clone

CAT#: RG210369

C4orf17 (tGFP-tagged) - Human chromosome 4 open reading frame 17 (C4orf17)

Product Images

Circular map for RG210369

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C4orf17
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210369 representing NM_032149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCAACCCCCCGACATCTGCTCTTCAGATCGAGGGCAAAGGCAGCCATATTATGGCTAGAAATG
  TAAGCTGCTTTCTAGTCAGGCACACCCCTCATCCCAGAAGAGTCTGCCACATCAAAGGCTTGAATAACAT
  TCCAATCTGTACTGTGAATGATGATGAGAATGCATTTGGAACATTGTGGGAAGTTGGCCAGTCTAACTAC
  TTAGAGAAGAACAGGATACCATTTGCCAATTGCAGTTACCCCCCCAGCACTGCAGTCCAGAAGAGCCCTG
  TAAGAGGAATGTCGCCAGCCCCAAACGGTGCCAAAGTGCCTCCACGGCCTCATTCTGAGCCCAGTAGAAA
  AATTAAAGAGTGCTTCAAAACTTCCAGTGAGAATCCCTTAGTAATTAAAAAGGAAGAAATTAAGGCCAAA
  AGACCACCATCACCTCCAAAGGCATGCTCTACTCCTGGCTCCTGTTCTTCAGGGATGACAAGTACCAAGA
  ATGATGTGAAAGCAAACACCATTTGCATACCAAACTATCTGGATCAGGAAATAAAAATCCTGGCAAAGCT
  CTGTAGCATTTTGCATACTGATTCTCTGGCAGAAGTTTTACAGTGGCTGCTTCATGCAACTTCAAAAGAA
  AAAGAGTGGGTCTCAGCTTTGATTCATTCTGAGCTTGCCGAGATAAACCTGTTAACTCATCACAGAAGAA
  ACACCTCAATGGAACCAGCAGCAGAGACTGGGAAGCCACCCACAGTTAAATCACCACCCACAGTTAAATT
  GCCCCCAAATTTTACTGCAAAATCAAAAGTGCTGACCAGAGATACAGAAGGGGATCAACCAACCAGAGTG
  TCAAGTCAAGGATCTGAAGAAAACAAGGAAGTACCAAAAGAGGCTGAGCACAAGCCTCCACTACTTATAA
  GAAGAAATAATATGAAAATACCTGTTGCAGAATATTTCAGCAAACCAAATTCTCCTCCCAGGCCTAACAC
  TCAGGAGAGTGGATCAGCAAAACCAGTGTCAGCAAGGAGTATACAAGAATACAACCTCTGTCCCCAAAGA
  GCATGTTATCCTTCAACACACCGGAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210369 representing NM_032149
  Red=Cloning site Green=Tags(s)
  
  MNLNPPTSALQIEGKGSHIMARNVSCFLVRHTPHPRRVCHIKGLNNIPICTVNDDENAFGTLWEVGQSNY
  LEKNRIPFANCSYPPSTAVQKSPVRGMSPAPNGAKVPPRPHSEPSRKIKECFKTSSENPLVIKKEEIKAK
  RPPSPPKACSTPGSCSSGMTSTKNDVKANTICIPNYLDQEIKILAKLCSILHTDSLAEVLQWLLHATSKE
  KEWVSALIHSELAEINLLTHHRRNTSMEPAAETGKPPTVKSPPTVKLPPNFTAKSKVLTRDTEGDQPTRV
  SSQGSEENKEVPKEAEHKPPLLIRRNNMKIPVAEYFSKPNSPPRPNTQESGSAKPVSARSIQEYNLCPQR
  ACYPSTHRR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032149
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032149.1, NP_115525.1
• RefSeq Size: 1613 bp
• RefSeq ORF: 1080 bp
• Locus ID: 84103
• UniProt ID: Q53FE4
• Cytogenetics: 4q23