TRF41 (PAPD7) (NM_006999) Human Tagged ORF Clone

CAT#: RG210285

PAPD7 (tGFP-tagged) - Human PAP associated domain containing 7 (PAPD7), transcript variant 1

Product Images

Circular map for RG210285

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRF41
• Synonyms: LAK-1; LAK1; PAPD7; POLK; POLS; TRF4; TRF4-1; TRF41; TUTASE5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210285 representing NM_006999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCTTGTCCTGAAGAAGCAGCTATGAGAAGAGAGGTGGTGAAACGGATCGAAACTGTGGTGAAAG
  ACCTTTGGCCGACGGCTGATGTACAGATATTTGGCAGCTTTAGTACAGGTCTTTATCTTCCAACTAGCGA
  CATAGACCTGGTGGTCTTCGGGAAATGGGAGCGTCCTCCTTTACAGCTGCTGGAGCAAGCCCTGCGGAAG
  CACAACGTGGCTGAGCCGTGTTCCATCAAAGTCCTTGACAAGGCTACGGTACCAATAATAAAGCTCACAG
  ATCAGGAGACTGAAGTGAAAGTTGACATCAGCTTTAACATGGAGACGGGCGTCCGGGCAGCGGAGTTCAT
  CAAGAATTACATGAAGAAATATTCATTGCTGCCTTACTTGATTTTAGTATTGAAACAGTTCCTTCTGCAG
  AGGGACCTGAATGAAGTTTTTACAGGTGGAATTAGCTCATACAGCCTAATTTTAATGGCCATTAGCTTTC
  TACAGTTGCATCCAAGAATTGATGCCCGGAGAGCTGATGAAAACCTTGGAATGCTTCTTGTAGAATTTTT
  TGAACTCTATGGGAGAAATTTTAATTACTTGAAAACCGGTATTAGAATCAAAGAAGGAGGTGCCTATATC
  GCCAAAGAGGAGATCATGAAAGCCATGACCAGCGGGTACAGACCGTCGATGCTGTGCATTGAGGACCCCC
  TGCTGCCAGGGAATGACGTTGGCCGGAGCTCCTATGGCGCCATGCAGGTGAAGCAGGTCTTCGATTATGC
  CTACATAGTGCTCAGCCATGCCGTGTCACCGCTGGCCAGGTCCTATCCAAACAGAGACGCCGAAAGTACT
  TTAGGAAGAATCATCAAAGTAACTCAGGAGGTGATTGACTACCGGAGGTGGATCAAAGAGAAGTGGGGCA
  GCAAAGCCCACCCGTCGCCAGGCATGGACAGCAGGATCAAGATCAAAGAGCGAATAGCCACATGCAATGG
  GGAGCAGACGCAGAACCGAGAGCCCGAGTCTCCCTATGGCCAGCGCTTGACTTTGTCGCTGTCCAGCCCC
  CAGCTCCTGTCTTCAGGCTCCTCGGCCTCTTCTGTGTCTTCACTTTCTGGGAGTGACGTTGATTCAGACA
  CACCGCCCTGCACAACGCCCAGTGTTTACCAGTTCAGTCTGCAAGCGCCAGCTCCTCTCATGGCCGGCTT
  ACCCACCGCCTTGCCAATGCCCAGTGGCAAACCTCAGCCCACCACTTCCAGAACACTGATCATGACAACC
  AACAATCAGACCAGGTTTACTATACCTCCACCGACCCTAGGGGTTGCTCCTGTTCCTTGCAGACAAGCTG
  GTGTAGAAGGAACTGCGTCTTTGAAAGCCGTCCACCACATGTCTTCCCCGGCCATTCCCTCAGCGTCCCC
  CAACCCGCTCTCGAGCCCTCATCTGTATCATAAGCAGCACAACGGCATGAAACTGTCCATGAAGGGCTCT
  CACGGCCACACCCAAGGCGGCGGCTACAGCTCTGTGGGTAGCGGAGGTGTGCGGCCCCCTGTGGGCAACA
  GGGGACACCACCAGTATAACCGCACCGGCTGGAGGAGGAAAAAACACACACACACACGGGACAGTCTGCC
  CGTGAGCCTCAGCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210285 representing NM_006999
  Red=Cloning site Green=Tags(s)
  
  MSPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPPLQLLEQALRK
  HNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETGVRAAEFIKNYMKKYSLLPYLILVLKQFLLQ
  RDLNEVFTGGISSYSLILMAISFLQLHPRIDARRADENLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYI
  AKEEIMKAMTSGYRPSMLCIEDPLLPGNDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRDAEST
  LGRIIKVTQEVIDYRRWIKEKWGSKAHPSPGMDSRIKIKERIATCNGEQTQNREPESPYGQRLTLSLSSP
  QLLSSGSSASSVSSLSGSDVDSDTPPCTTPSVYQFSLQAPAPLMAGLPTALPMPSGKPQPTTSRTLIMTT
  NNQTRFTIPPPTLGVAPVPCRQAGVEGTASLKAVHHMSSPAIPSASPNPLSSPHLYHKQHNGMKLSMKGS
  HGHTQGGGYSSVGSGGVRPPVGNRGHHQYNRTGWRRKKHTHTRDSLPVSLSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006999
• ORF Size: 1626 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006999.2
• RefSeq Size: 3861 bp
• RefSeq ORF: 2379 bp
• Locus ID: 11044
• UniProt ID: Q5XG87
• Cytogenetics: 5p15.31
• Domains: PAP_assoc, NTP_transf_2
• Protein Pathways: RNA degradation
• Gene Summary: The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jan 2010]