UGT2B11 (NM_001073) Human Tagged ORF Clone

CAT#: RG210178

UGT2B11 (tGFP-tagged) - Human UDP glucuronosyltransferase 2 family, polypeptide B11 (UGT2B11)

Product Images

Circular map for RG210178

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UGT2B11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210178 representing NM_001073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTGAAATGGACTTCAGTTCTTCTGCTGATACATCTCAGTTGTTACTTTAGCTCTGGGAGTTGTG
  GAAAAGTGCTGGTGTGGGCCGCAGAATACAGCCATTGGATGAATATGAAGACAATCCTGAAAGAGCTTGT
  TCAGAGAGGTCATGAGGTGACTGTACTGGCATCTTCAGCTTCCATTCTTTTTGATCCCAATGATGCATCC
  ACTCTTAAATTTGAAGTTTATCCTACATCTTTAACTAAAACTGAATTTGAGAATATCATCATGCAACAGG
  TTAAGAGATGGTCAGACATTCGAAAAGATAGCTTTTGGTTATATTTTTCACAAGAACAAGAAATCCTGTG
  GGAATTATATGACATATTTAGAAACTTCTGTAAAGATGTAGTTTCAAATAAGAAAGTTATGAAAAAACTA
  CAAGAGTCAAGATTTGACATCGTTTTTGCAGATGCTGTTTTTCCCTGTGGTGAGCTGCTGGCTGCGCTAC
  TTAACATACGGTTTGTGTACAGTCTCCGCTTTACTCCTGGCTACACAATTGAAAGGCACAGTGGAGGACT
  GATTTTCCCTCCTTCCTACATACCTATTGTTATGTCAAAATTAAGTGATCAAATGACTTTCATGGAGAGG
  GTAAAAAATATGATCTATGTGCTTTATTTTGACTTTTGGTTCCAAATGTCTGATATGAAGAAGTGGGATC
  AGTTTTACAGTGAAGTTTTAGGAAGACCCACTACCTTATTTGAGACAATGGGAAAAGCTGACATATGGCT
  TATGCGAAACTCCTGGAGTTTTCAATTTCCTCATCCATTCTTACCAAACGTTGATTTTGTTGGAGGATTC
  CACTGCAAACCTGCCAAACCCCTACCTAAGGAAATGGAGGAGTTTGTACAGAGCTCTGGAGAAAATGGTG
  TTGTGGTGTTTTCTCTGGGGTCAGTGATAAGTAACATGACAGCAGAAAGGGCCAATGTAATTGCAACAGC
  CCTTGCCAAGATCCCACAAAAGGTTCTGTGGAGATTTGACGGGAATAAACCAGATGCCTTAGGTCTCAAT
  ACTCGGCTGTACAAGTGGATACCCCAGAATGACCTTCTAGGTCATCCAAAAACCAGAGCTTTTATAACTC
  ATGGTGGAGCCAATGGCATCTATGAGGCAATCTACCATGGGATCCCTATGGTGGGCATTCCATTGTTTTT
  TGATCAACCTGATAACATTGCTCACATGAAGGCCAAGGGAGCAGCTGTTAGATTGGACTTCAACACAATG
  TCGAGTACAGACCTGCTGAATGCACTGAAGACAGTAATTAATGATCCTTTATATAAAGAGAATATTATGA
  AATTATCAAGAATTCAACATGATCAACCAGTAAAGCCCCTGGATCGAGCAGTCTTCTGGATTGAATTTGT
  CATGCCCCACAAAGGAGCCAAACACCTTCGAGTTGCAGCCCATGACCTCACCTGGTTCCAGTACCACTCT
  TTGGATGTGATTGGGTTTCTGCTGGCCTGTGTGGCAACTGTGATATTTATCATCACAAAGTTTTGTCTGT
  TTTGTTTCTGGAAGTTTGCTAGAAAAGGGAAGAAGGGAAAAAGAGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210178 representing NM_001073
  Red=Cloning site Green=Tags(s)
  
  MTLKWTSVLLLIHLSCYFSSGSCGKVLVWAAEYSHWMNMKTILKELVQRGHEVTVLASSASILFDPNDAS
  TLKFEVYPTSLTKTEFENIIMQQVKRWSDIRKDSFWLYFSQEQEILWELYDIFRNFCKDVVSNKKVMKKL
  QESRFDIVFADAVFPCGELLAALLNIRFVYSLRFTPGYTIERHSGGLIFPPSYIPIVMSKLSDQMTFMER
  VKNMIYVLYFDFWFQMSDMKKWDQFYSEVLGRPTTLFETMGKADIWLMRNSWSFQFPHPFLPNVDFVGGF
  HCKPAKPLPKEMEEFVQSSGENGVVVFSLGSVISNMTAERANVIATALAKIPQKVLWRFDGNKPDALGLN
  TRLYKWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVGIPLFFDQPDNIAHMKAKGAAVRLDFNTM
  SSTDLLNALKTVINDPLYKENIMKLSRIQHDQPVKPLDRAVFWIEFVMPHKGAKHLRVAAHDLTWFQYHS
  LDVIGFLLACVATVIFIITKFCLFCFWKFARKGKKGKRD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001073
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001073.3
• RefSeq Size: 1722 bp
• RefSeq ORF: 1590 bp
• Locus ID: 10720
• UniProt ID: O75310
• Cytogenetics: 4q13.2
• Domains: UDPGT
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• Gene Summary: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds.[UniProtKB/Swiss-Prot Function]