EXOSC10 (NM_001001998) Human Tagged ORF Clone

CAT#: RG210055

EXOSC10 (tGFP-tagged) - Human exosome component 10 (EXOSC10), transcript variant 1

Product Images

Circular map for RG210055

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EXOSC10
• Synonyms: p2; p3; p4; PM-Scl; PM/Scl-100; PMSCL; PMSCL2; RRP6; Rrp6p
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210055 representing NM_001001998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCACCCAGTACCCGGGAGCCCAGGGTCCTGTCGGCGACCAGCGCAACCAAATCCGACGGAGAGA
  TGGTGCTGCCAGGCTTCCCGGACGCCGACAGCTTTGTGAAGTTTGCTCTTGGGTCCGTGGTGGCAGTCAC
  CAAGGCATCTGGGGGCCTACCACAGTTTGGCGATGAGTATGATTTTTACCGAAGTTTTCCTGGCTTCCAA
  GCATTTTGCGAAACACAGGGAGACAGGTTGCTTCAGTGCATGAGCAGAGTAATGCAGTACCATGGGTGTC
  GCAGCAACATTAAGGATCGAAGTAAAGTGACTGAGCTGGAAGACAAGTTTGATTTACTAGTTGATGCCAA
  TGATGTAATTCTGGAGAGAGTGGGTATTTTACTGGATGAAGCCTCAGGTGTAAACAAGAATCAACAGCCT
  GTCCTCCCTGCCGGCTTGCAGGTCCCCAAAACGGTAGTGTCCAGCTGGAACCGTAAGGCAGCAGAATATG
  GCAAAAAAGCAAAATCTGAAACTTTCCGGCTGCTTCATGCAAAAAATATCATCCGACCTCAGCTCAAGTT
  TCGAGAGAAGATTGACAATTCCAACACACCATTTCTTCCTAAAATCTTCATCAAACCCAATGCTCAGAAA
  CCTCTCCCTCAAGCTCTCTCTAAGGAAAGGCGGGAACGCCCACAGGATCGTCCTGAGGACTTGGACGTCC
  CCCCTGCACTGGCTGATTTCATCCATCAGCAGAGAACCCAGCAGGTTGAGCAAGACATGTTTGCACATCC
  TTATCAATATGAACTAAATCACTTTACCCCAGCAGATGCAGTGCTTCAAAAGCCACAACCCCAGTTATAC
  AGACCTATAGAAGAGACACCATGCCATTTCATATCCTCCCTGGATGAACTCGTGGAACTCAACGAAAAGC
  TCTTGAATTGTCAGGAATTTGCAGTTGACTTGGAGCACCACTCTTACAGGAGCTTCCTGGGACTGACCTG
  CCTGATGCAAATTTCTACTCGGACGGAAGACTTCATCATTGACACCCTCGAGCTTCGAAGTGACATGTAC
  ATTCTCAATGAGAGCCTCACAGACCCAGCCATCGTTAAGGTCTTTCATGGTGCTGATTCAGACATAGAAT
  GGCTACAGAAAGACTTTGGGTTGTATGTAGTAAACATGTTTGATACTCATCAGGCAGCACGCCTTCTTAA
  CCTGGGCAGGCACTCACTCGATCATCTCCTGAAACTCTACTGCAACGTGGACTCAAACAAGCAATATCAG
  CTGGCTGATTGGAGAATACGCCCTCTGCCCGAGGAGATGCTCAGCTACGCCCGGGATGACACCCATTACC
  TGCTATATATCTATGACAAAATGAGGCTGGAGATGTGGGAGCGCGGCAACGGGCAGCCCGTGCAGCTGCA
  GGTGGTGTGGCAACGGAGCAGGGACATCTGCCTCAAGAAATTCATCAAACCTATCTTCACGGATGAGTCC
  TACCTTGAACTCTATAGGAAGCAGAAGAAGCACCTTAACACACAGCAGTTGACAGCCTTTCAGCTGCTGT
  TTGCCTGGAGGGATAAAACAGCTCGCAGGGAAGATGAAAGTTACGGATATGTACTGCCAAACCACATGAT
  GCTGAAAATAGCTGAAGAACTGCCTAAGGAACCTCAGGGCATCATAGCTTGCTGCAACCCAGTACCGCCC
  CTTGTGCGGCAGCAGATCAACGAAATGCACCTTTTAATCCAGCAGGCCCGAGAGATGCCCCTGCTCAAGT
  CTGAAGTTGCAGCCGGAGTGAAGAAGAGCGGACCGCTGCCCAGTGCTGAGAGATTGGAGAATGTTCTCTT
  TGGACCTCACGACTGCTCCCATGCCCCTCCGGATGGCTATCCAATCATCCCAACCAGTGGATCTGTGCCA
  GTTCAGAAGCAGGCGAGCCTCTTCCCTGATGAAAAAGAAGATAACTTGCTGGGTACCACATGCCTGATTG
  CCACAGCTGTCATCACGTTATTTAATGAACCTAGTGCTGAAGACAGTAAAAAGGGTCCATTGACAGTTGC
  ACAGAAAAAAGCCCAGAACATCATGGAGTCCTTTGAAAATCCATTTAGGATGTTTCTGCCCTCACTGGGA
  CACCGTGCTCCCGTCTCTCAGGCAGCGAAGTTCGATCCATCAACCAAAATCTATGAAATCAGCAACCGTT
  GGAAGCTGGCCCAGGTACAAGTACAAAAAGACTCTAAAGAAGCTGTCAAGAAGAAGGCAGCTGAGCAAAC
  AGCTGCCCGGGAACAGGCAAAGGAGGCGTGCAAAGCTGCAGCAGAACAGGCCATCTCCGTCCGACAGCAG
  GTCGTGCTAGAAAATGCTGCAAAGAAGAGAGAGCGAGCAACAAGCGACCCAAGGACCACAGAACAGAAAC
  AAGAGAAGAAACGACTCAAAATTTCCAAGAAGCCAAAGGACCCAGAGCCACCAGAAAAAGAGTTTACGCC
  TTACGACTACAGCCAGTCAGACTTCAAGGCTTTTGCTGGAAACAGCAAATCCAAAGTTTCTTCTCAGTTT
  GATCCAAATAAACAGACCCCGTCTGGCAAGAAATGCATTGCAGCCAAAAAAATTAAACAGTCGGTGGGAA
  ACAAAAGCATGTCCTTTCCAACTGGAAAGTCAGACAGAGGCTTCAGGTACAACTGGCCACAGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210055 representing NM_001001998
  Red=Cloning site Green=Tags(s)
  
  MAPPSTREPRVLSATSATKSDGEMVLPGFPDADSFVKFALGSVVAVTKASGGLPQFGDEYDFYRSFPGFQ
  AFCETQGDRLLQCMSRVMQYHGCRSNIKDRSKVTELEDKFDLLVDANDVILERVGILLDEASGVNKNQQP
  VLPAGLQVPKTVVSSWNRKAAEYGKKAKSETFRLLHAKNIIRPQLKFREKIDNSNTPFLPKIFIKPNAQK
  PLPQALSKERRERPQDRPEDLDVPPALADFIHQQRTQQVEQDMFAHPYQYELNHFTPADAVLQKPQPQLY
  RPIEETPCHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDFIIDTLELRSDMY
  ILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARLLNLGRHSLDHLLKLYCNVDSNKQYQ
  LADWRIRPLPEEMLSYARDDTHYLLYIYDKMRLEMWERGNGQPVQLQVVWQRSRDICLKKFIKPIFTDES
  YLELYRKQKKHLNTQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPP
  LVRQQINEMHLLIQQAREMPLLKSEVAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIPTSGSVP
  VQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQNIMESFENPFRMFLPSLG
  HRAPVSQAAKFDPSTKIYEISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAISVRQQ
  VVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQSDFKAFAGNSKSKVSSQF
  DPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYNWPQR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001998
• ORF Size: 2655 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001998.1, NP_001001998.1
• RefSeq Size: 2834 bp
• RefSeq ORF: 2658 bp
• Locus ID: 5394
• UniProt ID: Q01780
• Cytogenetics: 1p36.22
• Protein Pathways: RNA degradation
• Gene Summary: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.[UniProtKB/Swiss-Prot Function]