SF3B3 (NM_012426) Human Tagged ORF Clone

CAT#: RG209509

SF3B3 (tGFP-tagged) - Human splicing factor 3b, subunit 3, 130kDa (SF3B3)

Product Images

Circular map for RG209509

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SF3B3
• Synonyms: RSE1; SAP130; SF3b130; STAF130
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209509 representing NM_012426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTCATGGAAACTTTTCTG
  GAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGGAGCTGCTTCGCCCAGACCCCAACAC
  TGGCAAAGTACATACCCTACTCACTGTGGAAGTATTCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTG
  ACAGGTGGCACCAAAGACTACATTGTAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGC
  CATCTAAGAATATGTTTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCC
  TGGCCAGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGAAATTG
  GTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGGAAGCCCACAAAGCAA
  ACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAAATCCAATGTTTGCTTGTCTGGAAAT
  GGATTATGAGGAAGCAGACAATGATCCAACAGGGGAAGCAGCAGCTAATACCCAGCAGACACTTACTTTC
  TATGAGCTAGACCTTGGTTTAAATCATGTGGTCCGAAAATACAGTGAACCTTTGGAGGAACACGGCAACT
  TCCTTATTACAGTTCCAGGAGGGTCAGATGGTCCAAGTGGAGTACTGATCTGCTCTGAAAACTATATTAC
  TTACAAGAACTTTGGTGACCAGCCAGATATCCGCTGTCCAATTCCCAGGAGGCGGAATGACCTGGATGAC
  CCTGAAAGAGGAATGATTTTTGTCTGCTCTGCAACCCATAAAACCAAATCGATGTTCTTCTCTTTGGCTC
  AAACTGAGCAGGGAGATATCTTTAAGATCACTTTGGAGACAGATGAAGATATGGTTACTGAGATCCGGCT
  CAAATATTTTGATACTGTACCCGTTGCTGCTGCCATGTGTGTGCTTAAAACAGGGTTCCTTTTTGTAGCA
  TCAGAATTTGGAAACCATTACTTATATCAAATTGCACATCTTGGAGATGATGATGAAGAACCTGAGTTTT
  CATCAGCCATGCCTCTGGAAGAAGGAGACACATTCTTTTTTCAGCCAAGACCACTTAAAAACCTTGTGCT
  GGTTGATGAGTTGGACAGCCTCTCTCCCATTCTGTTTTGCCAGATAGCTGATCTGGCCAATGAAGATACT
  CCACAGTTGTATGTGGCCTGTGGTAGGGGACCCCGATCATCTCTGAGAGTCCTAAGACATGGACTTGAGG
  TGTCAGAAATGGCTGTTTCTGAGCTACCTGGTAACCCCAACGCTGTCTGGACAGTGCGTCGACACATTGA
  AGATGAGTTTGATGCCTACATTATTGTGTCTTTCGTGAATGCCACCCTAGTGTTGTCCATTGGAGAAACT
  GTAGAAGAAGTGACTGACTCTGGGTTCCTGGGGACCACCCCGACCTTGTCCTGCTCCTTATTAGGAGATG
  ATGCCTTGGTGCAGGTCTATCCAGATGGCATTCGGCACATACGAGCAGACAAGAGAGTCAATGAGTGGAA
  GACCCCTGGAAAGAAAACAATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATTGCCCTGACAGGA
  GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGCTGAATGAGTACACAGAACGGAAGGAGATGT
  CAGCAGATGTGGTGTGCATGAGTCTGGCCAATGTACCCCCTGGAGAGCAGCGGTCTCGCTTCCTGGCTGT
  GGGGCTTGTGGACAACACTGTCAGAATCATCTCCCTGGATCCCTCAGACTGTTTGCAACCTCTAAGCATG
  CAGGCTCTCCCAGCCCAGCCTGAGTCCTTGTGTATCGTGGAAATGGGTGGGACTGAGAAGCAGGATGAGC
  TGGGTGAGAGGGGCTCGATTGGCTTCCTATACCTGAATATTGGGCTACAGAACGGTGTGCTGCTGAGGAC
  TGTCTTGGACCCTGTCACTGGGGATTTGTCTGATACTCGCACTCGGTACCTGGGGTCCCGTCCTGTGAAG
  CTCTTCCGAGTCCGAATGCAAGGCCAGGAGGCAGTATTGGCCATGTCAAGCCGCTCATGGTTGAGCTATT
  CTTACCAATCTCGCTTCCATCTCACCCCACTGTCTTACGAGACACTGGAATTTGCATCGGGTTTTGCCTC
  GGAACAGTGTCCCGAGGGCATTGTGGCCATCTCCACCAACACCCTACGGATTTTGGCATTAGAGAAGCTC
  GGTGCTGTCTTCAATCAAGTAGCCTTCCCACTGCAGTACACACCCAGGAAATTTGTCATCCACCCTGAGA
  GTAACAACCTTATTATCATTGAAACGGACCACAATGCCTACACTGAGGCCACGAAAGCTCAGAGAAAGCA
  GCAGATGGCAGAGGAAATGGTGGAAGCAGCAGGGGAGGATGAGCGGGAGCTGGCCGCAGAGATGGCAGCA
  GCATTCCTCAATGAAAACCTCCCTGAATCCATCTTTGGAGCTCCCAAGGCTGGCAATGGGCAGTGGGCCT
  CTGTGATCCGAGTGATGAATCCCATTCAAGGGAACACACTGGACCTTGTCCAGCTGGAACAGAATGAGGC
  AGCTTTTAGTGTGGCTGTGTGCAGGTTTTCCAACACTGGTGAAGACTGGTATGTGCTGGTGGGTGTGGCC
  AAGGACCTGATACTAAACCCCCGATCTGTGGCAGGGGGCTTCGTCTATACTTACAAGCTTGTGAACAATG
  GGGAAAAACTGGAGTTTTTGCACAAGACTCCTGTGGAAGAGGTCCCTGCTGCTATTGCCCCATTCCAGGG
  GAGGGTGTTGATTGGTGTGGGGAAGCTGTTGCGTGTCTATGACCTGGGAAAGAAGAAGTTACTCCGAAAA
  TGTGAGAATAAGCATATTGCCAATTATATCTCTGGGATCCAGACTATCGGACATAGGGTAATTGTATCTG
  ATGTCCAAGAAAGTTTCATCTGGGTTCGCTACAAGCGTAATGAAAACCAGCTTATCATCTTTGCTGATGA
  TACCTACCCCCGATGGGTCACTACAGCCAGCCTCCTGGACTATGACACTGTGGCTGGGGCAGACAAGTTT
  GGCAACATATGTGTGGTGAGGCTCCCACCTAACACCAATGATGAAGTAGATGAGGATCCTACAGGAAACA
  AAGCCCTGTGGGACCGTGGCTTGCTCAATGGGGCCTCCCAGAAGGCAGAGGTGATCATGAACTACCATGT
  CGGGGAGACGGTGCTGTCCTTGCAGAAGACCACGCTGATCCCTGGAGGCTCAGAATCACTTGTCTATACC
  ACCTTGTCTGGAGGAATTGGCATCCTTGTGCCATTCACGTCCCATGAGGACCATGACTTCTTCCAGCATG
  TGGAAATGCACCTGCGGTCTGAACATCCCCCTCTCTGTGGGCGGGACCACCTCAGCTTTCGCTCCTACTA
  CTTCCCTGTGAAGAATGTGATTGATGGAGACCTCTGTGAGCAGTTCAATTCCATGGAACCCAACAAACAA
  AAGAACGTCTCTGAAGAACTGGACCGAACCCCACCCGAAGTGTCCAAGAAACTCGAGGATATCCGGACCC
  GCTACGCCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209509 representing NM_012426
  Red=Cloning site Green=Tags(s)
  
  MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRL
  TGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKL
  VYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTF
  YELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDD
  PERGMIFVCSATHKTKSMFFSLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVA
  SEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDT
  PQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGET
  VEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTG
  GELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSM
  QALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVK
  LFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKL
  GAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAA
  AFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVA
  KDLILNPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRK
  CENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKF
  GNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYT
  TLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQ
  KNVSEELDRTPPEVSKKLEDIRTRYAF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012426
• ORF Size: 3651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012426.3, NP_036558.3
• RefSeq Size: 4357 bp
• RefSeq ORF: 3654 bp
• Locus ID: 23450
• UniProt ID: Q15393
• Cytogenetics: 16q22.1
• Domains: CPSF_A
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]