PDXDC1 (NM_015027) Human Tagged ORF Clone

CAT#: RG209322

PDXDC1 (tGFP-tagged) - Human pyridoxal-dependent decarboxylase domain containing 1 (PDXDC1)

Product Images

Circular map for RG209322

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDXDC1
• Synonyms: LP8165
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209322 representing NM_015027
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGTCCCTGGAGAAGATAGCAGACCCCACGTTAGCTGAAATGGGAAAAAACTTGAAGGAGGCAG
  TGAAGATGCTGGAGGACAGTCAGAGAAGAACAGAAGAGGAAAATGGAAAGAAGCTCATATCCGGAGATAT
  TCCAGGCCCACTCCAGGGCAGTGGGCAAGATATGGTGAGCATCCTCCAGTTAGTTCAGAATCTCATGCAT
  GGAGATGAAGATGAGGAGCCCCAGAGCCCCAGAATCCAAAATATTGGAGAACAAGGTCATATGGCTTTGT
  TGGGACATAGTCTGGGAGCTTATATTTCAACTCTGGACAAAGAGAAGCTGAGAAAACTTACAACTAGGAT
  ACTTTCAGATACCACCTTATGGCTATGCAGAATTTTCAGATATGAAAATGGGTGTGCTTATTTCCACGAA
  GAGGAAAGAGAAGGACTTGCAAAGATATGTAGGCTTGCCATTCATTCTCGATATGAAGACTTCGTAGTGG
  ATGGCTTCAATGTGTTATATAACAAGAAGCCTGTCATATATCTTAGTGCTGCTGCTAGACCTGGCCTGGG
  CCAATACCTTTGTAATCAGCTCGGCTTGCCCTTCCCCTGCTTGTGCCGTGTACCCTGTAACACTGTGTTT
  GGATCCCAGCATCAGATGGATGTTGCCTTCCTGGAGAAACTGATTAAAGATGATATAGAGCGAGGAAGAC
  TGCCCCTGTTGCTTGTCGCAAATGCAGGAACGGCAGCAGTAGGACACACAGACAAGATTGGGAGATTGAA
  AGAACTCTGTGAGCAGTATGGCATATGGCTTCATGTGGAGGGTGTGAATCTGGCAACATTGGCTCTGGGT
  TATGTCTCCTCATCAGTGCTGGCTGCAGCCAAATGTGATAGCATGACGATGACTCCTGGCCCGTGGCTGG
  GTTTGCCAGCTGTTCCTGCGGTGACACTGTATAAACACGATGACCCTGCCTTGACTTTAGTTGCTGGTCT
  TACATCAAATAAGCCCACAGACAAACTCCGTGCCCTGCCTCTGTGGTTATCTTTACAATACTTGGGACTT
  GATGGGTTTGTGGAGAGGATCAAGCATGCCTGTCAACTGAGTCAACGGTTGCAGGAAAGTTTGAAGGAAG
  TGAATTACATCAAAATCTTGGTGGAAGATGAGCTCAGCTCCCCAGTGGTGGTGTTCAGATTTTTCCAGGA
  ATTACCAGGCTCAGATCCGGTGTTTAAAGCCGTCCCAGTGCCCAACATGACACCTTCAGGAGTCGGCCGG
  GAGAGGCACTCGTGTGACGCGCTGAATCGCTGGCTGGGAGAACAGCTGAAGCAGCTGGTGCCTGCAAGCG
  GCCTCACAGTCATGGATCTGGAAGCTGAGGGCACGTGTTTGCGGTTCAGCCCTTTGATGACCGCAGCAGT
  TTTAGGAACTCGGGGAGAGGATGTGGATCAGCTCGTAGCCTGCATAGAAAGCAAACTGCCAGTGCTGTGC
  TGTACGCTCCAGTTGCGTGAAGAGTTCAAGCAGGAAGTGGAAGCAACAGCAGGTCTCCTATATGTTGATG
  ACCCTAACTGGTCTGGAATAGGGGTTGTCAGGTATGAACATGCTAATGATGATAAGAGCAGTTTGAAATC
  AGATCCCGAAGGGGAAAACATCCATGCTGGACTCCTGAAGAAGTTAAATGAACTGGAATCTGACCTAACC
  TTTAAAATAGGCCCTGAGTATAAGAGCATGAAGAGCTGCCTTTATGTCGGCATGGCGAGCGACAACGTCG
  ATGCTGCTGAGCTCGTGGAGACCATTGCGGCCACAGCCCGGGAGATAGAGGAGAACTCGAGGCTTCTGGA
  AAACATGACAGAAGTGGTTCGGAAAGGCATTCAGGAAGCTCAAGTGGAGCTGCAGAAGGCAAGTGAAGAA
  CGGCTTCTGGAAGAGGGGGTGTTGCGGCAGATCCCTGTAGTGGGCTCCGTGCTGAATTGGTTTTCTCCGG
  TCCAGGCTTTACAGAAGGGAAGAACTTTTAACTTGACAGCAGGCTCTCTGGAGTCCACAGAACCCATATA
  TGTCTACAAAGCACAAGGTGCAGGAGTCACGCTGCCTCCAACGCCCTCGGGCAGTCGCACCAAGCAGAGG
  CTTCCAGGCCAGAAGCCTTTTAAAAGGTCCCTGCGAGGTTCAGATGCTTTGAGTGAGACCAGCTCAGTCA
  GTCACATTGAAGACTTAGAAAAGGTGGAGCGCCTATCCAGTGGGCCGGAGCAGATCACCCTCGAGGCCAG
  CAGCACTGAGGGACACCCAGGGGCTCCCAGCCCTCAGCACACCGACCAGACCGAGGCCTTCCAGAAAGGG
  GTCCCACACCCAGAAGATGACCACTCACAGGTAGAAGGACCGGAGAGCTTAAGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209322 representing NM_015027
  Red=Cloning site Green=Tags(s)
  
  MDASLEKIADPTLAEMGKNLKEAVKMLEDSQRRTEEENGKKLISGDIPGPLQGSGQDMVSILQLVQNLMH
  GDEDEEPQSPRIQNIGEQGHMALLGHSLGAYISTLDKEKLRKLTTRILSDTTLWLCRIFRYENGCAYFHE
  EEREGLAKICRLAIHSRYEDFVVDGFNVLYNKKPVIYLSAAARPGLGQYLCNQLGLPFPCLCRVPCNTVF
  GSQHQMDVAFLEKLIKDDIERGRLPLLLVANAGTAAVGHTDKIGRLKELCEQYGIWLHVEGVNLATLALG
  YVSSSVLAAAKCDSMTMTPGPWLGLPAVPAVTLYKHDDPALTLVAGLTSNKPTDKLRALPLWLSLQYLGL
  DGFVERIKHACQLSQRLQESLKEVNYIKILVEDELSSPVVVFRFFQELPGSDPVFKAVPVPNMTPSGVGR
  ERHSCDALNRWLGEQLKQLVPASGLTVMDLEAEGTCLRFSPLMTAAVLGTRGEDVDQLVACIESKLPVLC
  CTLQLREEFKQEVEATAGLLYVDDPNWSGIGVVRYEHANDDKSSLKSDPEGENIHAGLLKKLNELESDLT
  FKIGPEYKSMKSCLYVGMASDNVDAAELVETIAATAREIEENSRLLENMTEVVRKGIQEAQVELQKASEE
  RLLEEGVLRQIPVVGSVLNWFSPVQALQKGRTFNLTAGSLESTEPIYVYKAQGAGVTLPPTPSGSRTKQR
  LPGQKPFKRSLRGSDALSETSSVSHIEDLEKVERLSSGPEQITLEASSTEGHPGAPSPQHTDQTEAFQKG
  VPHPEDDHSQVEGPESLR
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015027
• ORF Size: 2364 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015027.1, NP_055842.1
• RefSeq Size: 4512 bp
• RefSeq ORF: 2367 bp
• Locus ID: 23042
• UniProt ID: Q6P996
• Cytogenetics: 16p13.11