ARPP21 (NM_016300) Human Tagged ORF Clone

CAT#: RG208661

ARPP21 (tGFP-tagged) - Human cAMP-regulated phosphoprotein, 21kDa (ARPP21), transcript variant 1

Product Images

Circular map for RG208661

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARPP21
• Synonyms: ARPP-21; R3HDM3; RCS; TARPP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208661 representing NM_016300
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGCAAGGAGACCTGAATCAGGCAATAGCAGAGGAAGGAGGGACTGAGCAGGAGACGGCCACTC
  CAGAGAACGGCATTGTTAAATCAGAAAGTCTGGATGAAGAGGAGAAACTGGAACTGCAGAGGCGGCTGGA
  GGCTCAGAATCAAGAAAGAAGAAAATCCAAGTCAGGAGCAGGAAAAGGTAAACTGACTCGCAGTCTTGCT
  GTCTGTGAGGAATCTTCTGCCAGACCAGGAGGTGAAAGTCTTCAGGATCAGGAATCAATTCATTTACAGC
  TTTCCAGTTTTTCCAGCCTGCAAGAGGAGGATAAATCTAGGAAAGATGACTCTGAAAGAGAAAAAGAAAA
  GGATAAAAACAAAGATAAAACCTCTGAAAAACCCAAGATCAGAATGTTATCAAAAGATTGCAGCCAAGAA
  TACACGGATTCTACAGGCATAGACTTACACGAGTTTCTGATTAACACATTAAAGAATAATTCCAGGGACA
  GGATGATACTTTTGAAAATGGAGCAGGAAATTATTGATTTCATTGCTGACAACAATAATCATTATAAAAA
  GTTCCCTCAGATGTCATCGTATCAGAGGATGCTTGTCCATCGAGTGGCAGCTTATTTTGGATTGGATCAC
  AATGTGGATCAAACAGGAAAATCTGTTATCATCAACAAGACCAGCAGCACCAGAATACCAGAGCAAAGGT
  TTTGTGAACATTTAAAAGATGAAAAAGGTGAAGAATCCCAGAAGCGGTTTATCTTGAAGCGAGATAACTC
  TAGTATTGATAAAGAAGACAATCAGCAAAACAGAATGCATCCATTTAGAGATGACAGACGAAGTAAATCA
  ATTGAAGAGAGAGAAGAGGAATATCAGAGAGTGAGGGAGAGAATATTTGCACACGATTCAGTTTGCTCCC
  AGGAAAGCCTTTTTGTGGAAAACAGTAGGCTCTTGGAAGACAGTAACATATGCAATGAGACCTATAAGAA
  AAGACAGCTCTTTCGGGGCAACAGAGATGGCTCAGGGAGAACATCTGGGAGTCGACAGAGCAGCTCAGAA
  AATGAACTCAAGTGGTCTGACCACCAAAGGGCCTGGAGCAGCACAGACTCCGACAGTTCCAACCGCAATC
  TAAAGCCCGCCATGACCAAGACGGCGAGTTTTGGGGGCATCACGGTGCTGACCAGGGGTGACAGCACTTC
  CAGTACTAGGAGTACCGGGAAGCTGTCCAAAGCAGGTTCCGAGTCTTCCAGCAGTGCAGGCTCCTCAGGA
  TCGCTGTCCCGCACCCATCCACCTCTCCAGAGCACACCCCTAGTCTCAGGTGTGGCAGCTGGCTCTCCAG
  GCTGTGTGCCTTATCCAGAGAATGGAATAGGGGGCCAGGTTGCTCCCAGCAGCACCAGCTACATCCTCCT
  TCCACTTGAAGCTGCAACAGGCATCCCGCCTGGAAGCATCCTTCTTAATCCACACACAGGCCAGCCCTTT
  GTGAATCCCGATGGAACTCCTGCAATATACAACCCACCCACCAGTCAGCAGCCCCTGCGAAGCGCCATGG
  TGGGGCAGTCCCAACAGCAGCCACCACAGCAGCAGCCCTCCCCGCAGCCCCAACAGCAGGTCCAGCCACC
  GCAGCCACAGATGGCAGGCCCTCTGGTCACTCAGAGAGATGATGTGGCAACACAGTTTGGCCAGATGACC
  CTGAGCCGGCAGTCCTCGGGGGAGACTCCTGAACCCCCATCAGGTCCTGTCTACCCATCCTCCCTTATGC
  CACAGCCGGCCCAGCAGCCCAGCTATGTAATCGCCTCTACAGGCCAGCAGCTTCCTACAGGAGGATTCTC
  AGGCTCTGGCCCTCCCATCTCCCAGCAGGTCCTCCAGCCCCCTCCCTCACCACAGGGATTTGTGCAACAG
  CCTCCGCCTGCACAGATGCCTGTATATTATTACCCATCTGGTCAGTACCCTACCTCAACCACGCAACAGT
  ACCGGCCCATGGCCCCGGTTCAGTACAACGCTCAGAGGAGTCAACAGATGCCACAGGCAGCACAGCAAGC
  AGGTTACCAGCCAGTCTTGTCTGGTCAACAGGGATTCCAAGGCCTAATAGGAGTGCAGCAGCCACCTCAG
  AGTCAGAACGTGATAAATAACCAACAAGGAACTCCGGTGCAAAGCGTGATGGTTTCCTACCCAACAATGT
  CTTCTTATCAGGTGCCAATGACCCAGGGTTCTCAAGGACTGCCCCAGCAGTCATACCAACAGCCAATCAT
  GCTACCTAACCAGGCAGGTCAAGGGTCACTCCCAGCCACTGGAATGCCTGTTTACTGTAATGTCACACCG
  CCCACCCCTCAGAACAACCTTAGGCTGATTGGCCCACACTGCCCCTCCAGCACTGTCCCAGTGATGTCAG
  CTAGCTGCAGAACAAACTGTGCAAGTATGAGCAATGCTGGTTGGCAGGTCAAATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208661 representing NM_016300
  Red=Cloning site Green=Tags(s)
  
  MSEQGDLNQAIAEEGGTEQETATPENGIVKSESLDEEEKLELQRRLEAQNQERRKSKSGAGKGKLTRSLA
  VCEESSARPGGESLQDQESIHLQLSSFSSLQEEDKSRKDDSEREKEKDKNKDKTSEKPKIRMLSKDCSQE
  YTDSTGIDLHEFLINTLKNNSRDRMILLKMEQEIIDFIADNNNHYKKFPQMSSYQRMLVHRVAAYFGLDH
  NVDQTGKSVIINKTSSTRIPEQRFCEHLKDEKGEESQKRFILKRDNSSIDKEDNQQNRMHPFRDDRRSKS
  IEEREEEYQRVRERIFAHDSVCSQESLFVENSRLLEDSNICNETYKKRQLFRGNRDGSGRTSGSRQSSSE
  NELKWSDHQRAWSSTDSDSSNRNLKPAMTKTASFGGITVLTRGDSTSSTRSTGKLSKAGSESSSSAGSSG
  SLSRTHPPLQSTPLVSGVAAGSPGCVPYPENGIGGQVAPSSTSYILLPLEAATGIPPGSILLNPHTGQPF
  VNPDGTPAIYNPPTSQQPLRSAMVGQSQQQPPQQQPSPQPQQQVQPPQPQMAGPLVTQRDDVATQFGQMT
  LSRQSSGETPEPPSGPVYPSSLMPQPAQQPSYVIASTGQQLPTGGFSGSGPPISQQVLQPPPSPQGFVQQ
  PPPAQMPVYYYPSGQYPTSTTQQYRPMAPVQYNAQRSQQMPQAAQQAGYQPVLSGQQGFQGLIGVQQPPQ
  SQNVINNQQGTPVQSVMVSYPTMSSYQVPMTQGSQGLPQQSYQQPIMLPNQAGQGSLPATGMPVYCNVTP
  PTPQNNLRLIGPHCPSSTVPVMSASCRTNCASMSNAGWQVKF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016300
• ORF Size: 2436 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016300.4, NP_057384.2
• RefSeq Size: 3241 bp
• RefSeq ORF: 2439 bp
• Locus ID: 10777
• UniProt ID: Q9UBL0
• Cytogenetics: 3p22.3
• Gene Summary: This gene encodes a cAMP-regulated phosphoprotein. The encoded protein is enriched in the caudate nucleus and cerebellar cortex. A similar protein in mouse may be involved in regulating the effects of dopamine in the basal ganglia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]