DHX8 (NM_004941) Human Tagged ORF Clone

CAT#: RG208403

DHX8 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8)

Product Images

Circular map for RG208403

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DHX8
• Synonyms: DDX8; Dhr2; HRH1; PRP22; PRPF22
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208403 representing NM_004941
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGGCTGTAGCCATGGCGGGAGCCTTAATCGGGTCGGAGCCAGGCCCCGCGGAAGAACTTGCCA
  AACTCGAGTACCTGTCTTTGGTGTCAAAGGTTTGCACTGAGCTGGACAATCACTTGGGGATCAACGACAA
  GGACCTTGCTGAATTTGTGATCAGTCTTGCTGAGAAAAATACCACCTTTGATACTTTTAAGGCTTCTCTC
  GTCAAAAATGGTGCAGAATTTACGGATTCTCTTATTAGTAACTTGCTGCGTCTCATACAAACCATGCGGC
  CTCCAGCGAAGCCTTCCACTAGCAAAGATCCAGTTGTTAAACCTAAAACAGAAAAAGAAAAGCTGAAGGA
  ACTCTTCCCAGTCCTTTGCCAACCGGACAACCCTTCTGTTCGGACCATGTTGGATGAAGATGATGTGAAA
  GTTGCTGTGGATGTCCTGAAAGAACTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAGGAGAAGCAAAGAG
  ATGCTGAACACCGGGACAGGACAAAGAAGAAGAAGCGGAGTCGAAGCCGAGATCGAAACCGAGATCGAGA
  CAGAGATAGGGAACGAAACCGAGATAGAGACCACAAGCGGAGACACCGATCCCGCTCTCGATCACGTTCC
  AGGACCCGGGAGAGGAATAAAGTGAAGTCTAGATATCGGTCCAGGAGCAGGAGTCAGAGTCCCCCCAAAG
  ACCGGAAGGACCGGGACAAATATGGAGAGCGGAATCTGGATAGATGGCGGGATAAGCATGTGGACCGCCC
  TCCTCCAGAAGAGCCCACCATTGGTGACATTTATAATGGCAAAGTTACCAGCATCATGCAGTTTGGTTGC
  TTTGTGCAGCTGGAAGGACTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAGCTCCGGCGGGAGG
  GTCGTGTGGCCAATGTAGCTGATGTCGTGAGCAAAGGCCAGAGGGTCAAAGTCAAAGTGCTGTCCTTCAC
  TGGGACCAAGACCAGCCTGAGCATGAAGGATGTGGATCAAGAGACTGGAGAAGATCTAAACCCAAATAGA
  CGGCGAAATCTTGTCGGGGAGACCAATGAGGAGACCTCAATGCGGAATCCTGATAGACCCACTCACTTGT
  CCCTTGTCAGTGCTCCTGAAGTAGAGGACGACTCACTGGAACGCAAGCGCCTCACCCAAATCTCTGACCC
  AGAGAAGTGGGAGATCAAACAGATGATTGCTGCCAATGTCCTTTCCAAAGAAGAATTTCCAGACTTTGAT
  GAAGAGACTGGCATTCTCCCTAAGGTGGATGATGAAGAAGATGAGGACCTTGAGATTGAATTGGTTGAGG
  AAGAGCCTCCATTCCTGAGAGGGCACACTAAGCAAAGCATGGACATGAGCCCCATTAAAATTGTCAAGAA
  CCCAGACGGCTCCCTCTCCCAAGCAGCAATGATGCAGAGTGCCTTGGCCAAAGAAAGGCGGGAACTCAAA
  CAGGCCCAGCGGGAAGCTGAGATGGATTCTATTCCCATGGGACTCAACAAACACTGGGTTGACCCTCTGC
  CTGATGCGGAAGGCAGACAGATTGCTGCCAACATGAGGGGTATTGGGATGATGCCCAATGATATTCCTGA
  GTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCCTCTTACGGAAAAAAGACCCAGATGTCAATCCTTGAG
  CAGAGGGAGAGCCTGCCCATCTACAAACTGAAGGAGCAATTGGTCCAGGCCGTCCATGACAATCAGATCC
  TGATTGTCATTGGTGAGACAGGATCTGGAAAGACAACACAGATCACCCAGTACCTGGCGGAGGCAGGCTA
  CACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCGGTGGCCAAAAGAGTG
  TCAGAGGAGTTTGGTTGTTGCTTAGGCCAAGAGGTGGGCTACACCATTCGATTTGAGGACTGCACTAGCC
  CTGAAACAGTCATCAAGTACATGACAGATGGGATGTTGCTTAGAGAGTGCTTGATTGACCCTGACCTCAC
  TCAGTACGCGATCATCATGTTGGACGAGGCACATGAGAGGACAATTCACACTGATGTGCTCTTTGGATTG
  TTGAAAAAGACAGTTCAGAAACGGCAGGACATGAAGCTGATTGTCACCTCAGCCACCTTGGATGCAGTGA
  AGTTTTCTCAATACTTCTATGAAGCTCCCATTTTCACCATCCCAGGTCGAACATATCCAGTGGAAATACT
  GTACACAAAGGAACCTGAGACAGATTATCTGGATGCCAGCCTGATTACTGTTATGCAGATTCATTTAACA
  GAACCACCAGGTGATATCCTGGTCTTCCTGACTGGTCAGGAAGAAATTGATACTGCTTGTGAGATCCTGT
  ATGAAAGAATGAAATCCCTGGGACCTGATGTTCCAGAGTTAATTATCCTCCCAGTGTACTCTGCTCTTCC
  CAGTGAGATGCAGACCCGAATCTTTGACCCAGCTCCACCAGGCAGCAGAAAGGTTGTGATTGCCACCAAT
  ATCGCAGAGACATCGCTGACTATTGATGGTATCTACTATGTGGTGGACCCAGGATTCGTGAAACAGAAAG
  TTTACAATTCCAAGACAGGGATTGACCAGCTCGTGGTGACGCCTATTTCTCAGGCTCAGGCAAAGCAACG
  AGCTGGCAGAGCTGGGAGAACAGGCCCAGGGAAGTGTTACAGGTTGTACACAGAACGTGCCTACCGAGAT
  GAAATGCTGACCACCAACGTGCCGGAAATCCAGAGAACCAACTTAGCAAGCACAGTGCTGTCACTCAAGG
  CCATGGGTATCAATGATCTGCTGTCCTTTGATTTCATGGATGCCCCACCTATGGAAACTTTGATCACAGC
  CATGGAGCAGCTGTACACACTGGGGGCCCTGGATGACGAGGGCCTGCTCACTCGCTTGGGCCGCCGGATG
  GCAGAGTTCCCTCTGGAGCCAATGCTATGCAAAATGCTCATCATGTCTGTGCATCTGGGCTGCAGTGAGG
  AAATGCTGACCATTGTATCCATGCTGTCTGTGCAGAACGTCTTCTATAGGCCCAAGGATAAACAAGCCCT
  TGCAGATCAGAAGAAGGCCAAATTCCACCAGACTGAAGGGGACCACCTCACCCTGCTAGCTGTGTACAAC
  TCCTGGAAGAACAACAAGTTCTCCAACCCATGGTGCTATGAGAACTTTATCCAGGCTCGTTCCCTGCGCC
  GGGCCCAGGACATTCGCGAGCAGATGTTAGGCATAATGGACAGACACAAGCTGGATGTTGTTTCCTGTGG
  CAAGTCCACAGTCCGAGTGCAGAAGGCCATCTGCAGTGGGTTCTTCCGTAATGCTGCCAAGAAAGACCCG
  CAGGAGGGTTACCGGACACTGATCGACCAGCAGGTGGTCTATATCCATCCTTCCAGTGCCCTCTTCAACA
  GACAGCCAGAATGGGTGGTGTACCATGAGCTGGTGCTCACCACCAAGGAATACATGCGTGAAGTTACCAC
  CATCGACCCTCGGTGGCTTGTGGAGTTTGCCCCAGCCTTCTTCAAGGTCTCAGACCCAACTAAGCTAAGC
  AAACAGAAGAAGCAACAGCGTCTTGAACCCTTGTACAACCGCTATGAGGAACCCAATGCCTGGAGAATAT
  CTCGAGCTTTCCGACGGCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208403 representing NM_004941
  Red=Cloning site Green=Tags(s)
  
  MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASL
  VKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVK
  VAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRS
  RTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGC
  FVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNR
  RRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTQISDPEKWEIKQMIAANVLSKEEFPDFD
  EETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELK
  QAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILE
  QRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRV
  SEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGL
  LKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLT
  EPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATN
  IAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRD
  EMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRM
  AEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYN
  SWKNNKFSNPWCYENFIQARSLRRAQDIREQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDP
  QEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLS
  KQKKQQRLEPLYNRYEEPNAWRISRAFRRR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004941
• ORF Size: 3660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004941.1, NP_004932.1
• RefSeq Size: 4201 bp
• RefSeq ORF: 3663 bp
• Locus ID: 1659
• UniProt ID: Q14562
• Cytogenetics: 17q21.31
• Domains: DEAD, helicase_C, S1, HA2
• Protein Pathways: Spliceosome
• Gene Summary: This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]