SLC44A1 (NM_080546) Human Tagged ORF Clone

CAT#: RG208237

SLC44A1 (tGFP-tagged) - Human solute carrier family 44, member 1 (SLC44A1)

Product Images

Circular map for RG208237

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC44A1
• Synonyms: CD92; CDW92; CHTL1; CONATOC; CTL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208237 representing NM_080546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCTGCAGCTCCGCCTCCTCCGCCGCGCAGAGCTCCAAACGAGAATGGAAGCCGCTGGAGGACC
  GTAGCTGCACAGACATACCATGGCTGCTGCTCTTCATCCTCTTCTGCATTGGGATGGGATTTATTTGTGG
  CTTTTCAATAGCAACAGGTGCAGCAGCAAGACTAGTGTCAGGATACGACAGCTATGGAAATATCTGTGGG
  CAGAAAAATACAAAGTTGGAAGCAATACCAAACAGTGGCATGGACCACACCCAGCGGAAGTATGTATTCT
  TTTTGGATCCATGCAACCTGGACTTGATAAACCGGAAGATTAAGTCTGTAGCACTGTGTGTAGCAGCGTG
  TCCAAGGCAAGAACTGAAAACTCTGAGTGATGTTCAGAAGTTTGCAGAGATAAATGGTTCAGCCCTATGT
  AGCTACAACCTAAAGCCTTCTGAATACACTACATCTCCAAAATCTTCTGTTCTCTGCCCCAAACTACCAG
  TTCCAGCGAGTGCACCTATTCCATTCTTCCATCGCTGTGCTCCTGTGAACATTTCCTGCTATGCCAAGTT
  TGCAGAGGCCCTGATCACCTTTGTCAGTGACAATAGTGTCTTACACAGGCTGATTAGTGGAGTAATGACC
  AGCAAAGAAATTATATTGGGACTTTGCTTGTTATCACTAGTTCTATCCATGATTTTGATGGTGATAATCA
  GGTATATATCAAGAGTACTTGTGTGGATCTTAACGATTCTGGTCATACTCGGTTCACTTGGAGGCACAGG
  TGTACTATGGTGGCTGTATGCAAAGCAAAGAAGGTCTCCCAAAGAAACTGTTACTCCTGAGCAGCTTCAG
  ATAGCTGAAGACAATCTTCGGGCCCTCCTCATTTATGCCATTTCAGCTACAGTGTTCACAGTGATCTTAT
  TCCTGATAATGTTGGTTATGCGCAAACGTGTTGCTCTTACCATCGCCTTGTTCCACGTAGCTGGCAAGGT
  CTTCATTCACTTGCCACTGCTAGTCTTCCAACCCTTCTGGACTTTCTTTGCTCTTGTCTTGTTTTGGGTG
  TACTGGATCATGACACTTCTTTTTCTTGGCACTACCGGCAGTCCTGTTCAGAATGAGCAAGGCTTTGTGG
  AGTTCAAAATTTCTGGGCCTCTGCAGTACATGTGGTGGTACCATGTGGTGGGCCTGATTTGGATCAGTGA
  ATTTATTCTAGCATGTCAGCAGATGACAGTGGCAGGAGCTGTGGTAACATACTATTTTACTAGGGATAAA
  AGGAATTTGCCATTTACACCTATTTTGGCATCAGTAAATCGCCTTATTCGTTACCACCTAGGTACGGTGG
  CAAAAGGATCTTTCATTATCACATTAGTCAAAATTCCGCGAATGATCCTTATGTATATTCACAGTCAGCT
  CAAAGGAAAGGAAAATGCTTGTGCACGATGTGTGCTGAAATCTTGCATTTGTTGCCTTTGGTGTCTTGAA
  AAGTGCCTAAATTATTTAAATCAGAATGCATACACAGCCACAGCTATCAACAGCACCAACTTCTGCACCT
  CAGCAAAGGATGCCTTTGTCATTCTGGTGGAGAATGCTTTGCGAGTGGCTACCATCAACACAGTAGGAGA
  TTTTATGTTATTCCTTGGCAAGGTGCTGATAGTCTGCAGCACAGGTTTAGCTGGGATTATGCTGCTCAAC
  TACCAGCAGGACTACACAGTATGGGTGCTGCCTCTGATCATCGTCTGCCTCTTTGCTTTCCTAGTCGCTC
  ATTGCTTCCTGTCTATTTATGAAATGGTAGTGGATGTATTATTCTTGTGTTTTGCCATTGATACAAAATA
  CAATGATGGGAGCCCTGGCAGAGAATTCTATATGGATAAAGTGCTGATGGAGTTTGTGGAAAACAGTAGG
  AAAGCAATGAAAGAAGCTGGTAAGGGAGGCGTCGCTGATTCCAGAGAGCTAAAGCCGATGGCTTCGGGAG
  CAAGTTCTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208237 representing NM_080546
  Red=Cloning site Green=Tags(s)
  
  MGCCSSASSAAQSSKREWKPLEDRSCTDIPWLLLFILFCIGMGFICGFSIATGAAARLVSGYDSYGNICG
  QKNTKLEAIPNSGMDHTQRKYVFFLDPCNLDLINRKIKSVALCVAACPRQELKTLSDVQKFAEINGSALC
  SYNLKPSEYTTSPKSSVLCPKLPVPASAPIPFFHRCAPVNISCYAKFAEALITFVSDNSVLHRLISGVMT
  SKEIILGLCLLSLVLSMILMVIIRYISRVLVWILTILVILGSLGGTGVLWWLYAKQRRSPKETVTPEQLQ
  IAEDNLRALLIYAISATVFTVILFLIMLVMRKRVALTIALFHVAGKVFIHLPLLVFQPFWTFFALVLFWV
  YWIMTLLFLGTTGSPVQNEQGFVEFKISGPLQYMWWYHVVGLIWISEFILACQQMTVAGAVVTYYFTRDK
  RNLPFTPILASVNRLIRYHLGTVAKGSFIITLVKIPRMILMYIHSQLKGKENACARCVLKSCICCLWCLE
  KCLNYLNQNAYTATAINSTNFCTSAKDAFVILVENALRVATINTVGDFMLFLGKVLIVCSTGLAGIMLLN
  YQQDYTVWVLPLIIVCLFAFLVAHCFLSIYEMVVDVLFLCFAIDTKYNDGSPGREFYMDKVLMEFVENSR
  KAMKEAGKGGVADSRELKPMASGASSA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080546
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080546.5
• RefSeq Size: 4540 bp
• RefSeq ORF: 1974 bp
• Locus ID: 23446
• UniProt ID: Q8WWI5
• Cytogenetics: 9q31.1-q31.2
• Domains: DUF580
• Protein Families: Transmembrane
• Gene Summary: Choline transporter. May be involved in membrane synthesis and myelin production.[UniProtKB/Swiss-Prot Function]