TMEM199 (NM_152464) Human Tagged ORF Clone

CAT#: RG207692

TMEM199 (tGFP-tagged) - Human transmembrane protein 199 (TMEM199)

Product Images

Circular map for RG207692

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMEM199
• Synonyms: C17orf32; CDG2P; VMA12; VPH2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207692 representing NM_152464
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTCTTTGCTTGCGGGCGAGCGATTGGTGCGTGCTTTGGGCCCCGGCGGGGAGCTGGAGCCAG
  AGCGGCTACCCCGAAAGCTGCGGGCCGAGCTTGAGGCCGCGCTGGGGAAGAAGCACAAGGGCGGTGATAG
  CTCCAGTGGCCCCCAACGCTTGGTTTCTTTCCGTCTCATCCGGGATCTGCACCAGCATCTGAGAGAAAGG
  GATTCCAAACTATACCTCCATGAGCTCCTAGAAGGCAGTGAAATCTATCTCCCAGAGGTTGTGAAGCCTC
  CACGGAACCCAGAACTAGTTGCCCGGCTGGAGAAGATTAAGATACAGCTGGCCAATGAGGAATATAAACG
  GATCACCCGCAACGTCACTTGTCAGGATACAAGACATGGTGGGACTCTCAGCGACCTGGGAAAGCAAGTG
  AGATCATTGAAGGCTCTGGTCATCACCATCTTCAATTTCATTGTCACGGTGGTTGCTGCCTTCGTCTGCA
  CTTACCTTGGAAGCCAATATATCTTCACAGAAATGGCCTCGCGGGTGCTAGCTGCATTGATCGTCGCCTC
  TGTGGTGGGTCTGGCCGAGCTGTATGTCATGGTGCGGGCAATGGAAGGCGAGCTGGGAGAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207692 representing NM_152464
  Red=Cloning site Green=Tags(s)
  
  MASSLLAGERLVRALGPGGELEPERLPRKLRAELEAALGKKHKGGDSSSGPQRLVSFRLIRDLHQHLRER
  DSKLYLHELLEGSEIYLPEVVKPPRNPELVARLEKIKIQLANEEYKRITRNVTCQDTRHGGTLSDLGKQV
  RSLKALVITIFNFIVTVVAAFVCTYLGSQYIFTEMASRVLAALIVASVVGLAELYVMVRAMEGELGEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152464
• ORF Size: 624 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152464.3
• RefSeq Size: 1466 bp
• RefSeq ORF: 627 bp
• Locus ID: 147007
• UniProt ID: Q8N511
• Cytogenetics: 17q11.2
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) in some human cells. The encoded protein shares some homology with the yeast protein Vma12. Defects in this gene are a cause of congenital disorder of glycosylation, type IIp. [provided by RefSeq, Mar 2016]