TCP11 (NM_018679) Human Tagged ORF Clone

CAT#: RG207499

TCP11 (tGFP-tagged) - Human t-complex 11 homolog (mouse) (TCP11), transcript variant 2

Product Images

Circular map for RG207499

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TCP11
• Synonyms: D6S230E; FPPR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207499 representing NM_018679
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCAAAAGGCATATTGGGGTCATTTCCAACAGCTATGAACCTGAGTCTGGAAGGCAAGGTCAAGG
  AGACAGTGCACAATGCCTTTTGGGACCATCTTAAAGAGCAACTATCAGCAACTCCCCCTGACTTCAGCTG
  TGCTCTTGAACTTCTGAAAGAAATTAAAGAGATCTTGCTATCACTGCTATTACCACGCCAGAACCGCCTG
  AGAATTGAGATTGAAGAAGCTCTGGACATGGACTTGCTCAAGCAGGAGGCAGAACATGGGGCCCTGAAAG
  TCCTCTATCTCTCTAAGTACGTTCTCAACATGATGGCTTTGCTGTGTGCACCAGTTCGAGATGAAGCAGT
  GCAGAAACTAGAAAACATTACGGATCCTGTTTGGCTACTGAGAGGGATCTTCCAGGTTCTGGGCCGGATG
  AAAATGGACATGGTGAACTACACTATCCAGAGCCTTCAACCCCACCTGCAGGAACATTCCATTCAGTATG
  AACGGGCTAAATTCCAGGAACTCCTCAATAAGCAGCCTAGTCTCCTTAATCACACCACCAAATGGCTGAC
  CCAAGCAGCAGGAGACCTCACCATGTCACCTCCGACTTGCCCAGACACTTCTGACTCCTCCAGTGTGGCT
  GGCCCCTCTCCCAATGAGGCAGCCAACAACCCAGAGCCCCTCAGCCCCACAATGGTGCTGTGTCAGGGCT
  TCTTGAACCTCCTTCTCTGGGACCTTGAAAATGAAGAGTTCCCTGAGACCCTGCTGATGGACAGAACCCG
  GCTGCAGGAGCTGAAGTCCCAGTTGCACCAGTTAACCGTCATGGCCTCAGTCTTGCTGGTGGCCAGTAGT
  TTCTCCGGCAGTGTTTTGTTTGGCTCACCCCAATTTGTAGATAAACTGAAACGCATAACCAAATCCTTGT
  TGGAAGACTTTCACTCCAGGCCTGAGGAAGCTATACTGACTGTGAGTGAACAGGTATCTCAGGAAATCCA
  TCAAAGCCTCAAGAATATGGGCCTTGTTGCTCTAAGCAGTGATAATACAGCATCTCTAATGGGACAGCTC
  CAGAACATTGCCAAGAAGGAGAACTGTGTCTGCAGTGTTATTGATCAGCGGATCCATTTGTTTCTCAAAT
  GCTGTTTGGTTCTTGGTGTGCAGCGGTCTCTATTAGACCTTCCTGGAGGCCTTACTCTCATTGAAGCAGA
  ACTGGCAGAACTGGGCCAAAAGTTTGTCAACTTGACACATCACAATCAGCAGGTGTTTGGTCCCTACTAC
  ACTGAGATCCTAAAAACCCTCATTTCCCCAGCCCAGGCACTGGAAACAAAAGTGGAGTCTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207499 representing NM_018679
  Red=Cloning site Green=Tags(s)
  
  MAPKGILGSFPTAMNLSLEGKVKETVHNAFWDHLKEQLSATPPDFSCALELLKEIKEILLSLLLPRQNRL
  RIEIEEALDMDLLKQEAEHGALKVLYLSKYVLNMMALLCAPVRDEAVQKLENITDPVWLLRGIFQVLGRM
  KMDMVNYTIQSLQPHLQEHSIQYERAKFQELLNKQPSLLNHTTKWLTQAAGDLTMSPPTCPDTSDSSSVA
  GPSPNEAANNPEPLSPTMVLCQGFLNLLLWDLENEEFPETLLMDRTRLQELKSQLHQLTVMASVLLVASS
  FSGSVLFGSPQFVDKLKRITKSLLEDFHSRPEEAILTVSEQVSQEIHQSLKNMGLVALSSDNTASLMGQL
  QNIAKKENCVCSVIDQRIHLFLKCCLVLGVQRSLLDLPGGLTLIEAELAELGQKFVNLTHHNQQVFGPYY
  TEILKTLISPAQALETKVESV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018679
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018679.5, NP_061149.1
• RefSeq Size: 2035 bp
• RefSeq ORF: 1326 bp
• Locus ID: 6954
• UniProt ID: Q8WWU5
• Cytogenetics: 6p21.31
• Gene Summary: Plays a role in the process of sperm capacitation and acrosome reactions. Probable receptor for the putative fertilization-promoting peptide (FPP) at the sperm membrane that may modulate the activity of the adenylyl cyclase cAMP pathway.[UniProtKB/Swiss-Prot Function]