IGSF11 (NM_152538) Human Tagged ORF Clone

CAT#: RG206735

IGSF11 (tGFP-tagged) - Human immunoglobulin superfamily, member 11 (IGSF11), transcript variant 1

Product Images

Circular map for RG206735

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IGSF11
• Synonyms: BT-IgSF; CT119; CXADRL1; Igsf13; VSIG3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206735 representing NM_152538
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGGTGGAACTTTTGCTCTGGTGGAACTGCTTTTCTAGAACTGGTGTTGCAGCATCCCTGGAAG
  TGTCAGAGAGCCCTGGGAGTATCCAGGTGGCCCGGGGTCAGACAGCAGTCCTGCCCTGCACTTTCACTAC
  CAGCGCTGCCCTCATTAACCTCAATGTCATTTGGATGGTCACTCCTCTCTCCAATGCCAACCAACCTGAA
  CAGGTCATCCTGTATCAGGGTGGACAGATGTTTGATGGTGCCCCCCGGTTCCACGGTAGGGTAGGATTTA
  CAGGCACCATGCCAGCTACCAATGTCTCTATCTTCATTAATAACACTCAGTTATCAGACACTGGCACCTA
  CCAGTGCCTGGTCAACAACCTTCCAGACATAGGGGGCAGGAACATTGGGGTCACCGGTCTCACAGTGTTA
  GTTCCCCCTTCTGCCCCACACTGCCAAATCCAAGGATCCCAGGATATTGGCAGCGATGTCATCCTGCTCT
  GTAGCTCAGAGGAAGGCATTCCTCGACCAACTTACCTTTGGGAGAAGTTAGACAATACCCTCAAACTACC
  TCCAACAGCTACTCAGGACCAGGTCCAGGGAACAGTCACCATCCGGAACATCAGTGCCCTGTCTTCAGGT
  TTGTACCAGTGCGTGGCTTCTAATGCTATTGGAACCAGCACCTGTCTTCTGGATCTCCAGGTTATTTCAC
  CCCAGCCCAGGAACATTGGACTAATAGCTGGAGCCATTGGCACTGGTGCAGTTATTATCATTTTTTGCAT
  TGCACTAATTTTAGGGGCATTCTTTTACTGGAGAAGCAAAAATAAAGAGGAGGAAGAAGAAGAAATTCCT
  AATGAAATAAGAGAGGATGATCTTCCACCCAAGTGTTCTTCTGCCAAAGCATTTCACACTGAGATTTCCT
  CCTCGGACAACAACACACTAACCTCTTCCAATGCCTACAACAGTCGATACTGGAGCAACAATCCAAAAGT
  TCATAGAAACACAGAGTCAGTCAGCCACTTCAGTGACTTGGGCCAATCTTTCTCTTTCCACTCAGGCAAT
  GCCAACATACCATCCATTTATGCTAATGGGACCCATCTGGTCCCGGGTCAACATAAGACTCTGGTAGTGA
  CAGCCAACAGAGGGTCATCACCACAGGTGATGTCCAGGAGCAATGGCTCAGTCAGTAGGAAGCCTCGGCC
  TCCACACACTCATTCCTACACCATCAGCCACGCAACACTGGAACGAATTGGTGCAGTACCTGTCATGGTA
  CCAGCCCAGAGTCGGGCCGGGTCCTTGGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206735 representing NM_152538
  Red=Cloning site Green=Tags(s)
  
  MSLVELLLWWNCFSRTGVAASLEVSESPGSIQVARGQTAVLPCTFTTSAALINLNVIWMVTPLSNANQPE
  QVILYQGGQMFDGAPRFHGRVGFTGTMPATNVSIFINNTQLSDTGTYQCLVNNLPDIGGRNIGVTGLTVL
  VPPSAPHCQIQGSQDIGSDVILLCSSEEGIPRPTYLWEKLDNTLKLPPTATQDQVQGTVTIRNISALSSG
  LYQCVASNAIGTSTCLLDLQVISPQPRNIGLIAGAIGTGAVIIIFCIALILGAFFYWRSKNKEEEEEEIP
  NEIREDDLPPKCSSAKAFHTEISSSDNNTLTSSNAYNSRYWSNNPKVHRNTESVSHFSDLGQSFSFHSGN
  ANIPSIYANGTHLVPGQHKTLVVTANRGSSPQVMSRSNGSVSRKPRPPHTHSYTISHATLERIGAVPVMV
  PAQSRAGSLV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152538
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152538.1
• RefSeq Size: 3577 bp
• RefSeq ORF: 1293 bp
• Locus ID: 152404
• UniProt ID: Q5DX21
• Cytogenetics: 3q13.32
• Domains: ig, IGv, IGc2, IG
• Protein Families: Transmembrane
• Gene Summary: IGSF11 is an immunoglobulin (Ig) superfamily member that is preferentially expressed in brain and testis. It shares significant homology with coxsackievirus and adenovirus receptor (CXADR; MIM 602621) and endothelial cell-selective adhesion molecule (ESAM).[supplied by OMIM, Apr 2005]