P2X6 (P2RX6) (NM_005446) Human Tagged ORF Clone

CAT#: RG206536

P2RX6 (tGFP-tagged) - Human purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), transcript variant 1

Product Images

Circular map for RG206536

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: P2X6
• Synonyms: P2RXL1; P2X6; P2XM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206536 representing NM_005446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCCGCAGCTAGCAGGAGCTGGCAGCATGGGCTCCCCAGGGGCTACGACAGGCTGGGGGCTTCTGG
  ATTATAAGACGGAGAAGTATGTGATGACCAGGAACTGGCGGGTGGGCGCCCTGCAGAGGCTGCTGCAGTT
  TGGGATCGTGGTCTATGTGGTAGGGTGGGCTCTCCTCGCCAAAAAAGGCTACCAGGAGCGGGACCTGGAA
  CCCCAGTTTTCCATCATCACCAAACTCAAAGGGGTTTCCGTCACTCAGATCAAGGAGCTTGGAAACCGGC
  TGTGGGATGTGGCCGACTTCGTGAAGCCACCTCAGGGAGAGAACGTGTTCTTCTTGGTGACCAACTTCCT
  TGTGACGCCAGCCCAAGTTCAGGGCAGATGCCCAGAGCACCCGTCCGTCCCACTGGCTAACTGCTGGGTC
  GACGAGGACTGCCCCGAAGGGGAGGGAGGCACACACAGCCACGGTGTAAAAACAGGCCAGTGTGTGGTGT
  TCAATGGGACCCACAGGACCTGTGAGATCTGGAGTTGGTGCCCAGTGGAGAGTGGCGTTGTGCCCTCGAG
  GCCCCTGCTGGCCCAGGCCCAGAACTTCACACTGTTCATCAAAAACACAGTCACCTTCAGCAAGTTCAAC
  TTCTCTAAGTCCAATGCCTTGGAGACCTGGGACCCCACCTATTTTAAGCACTGCCGCTATGAACCACAAT
  TCAGCCCCTACTGTCCCGTGTTCCGCATTGGGGACCTCGTGGCCAAGGCTGGAGGGACCTTCGAGGACCT
  GGCGTTGCTGGGTGGCTCTGTAGGCATCAGAGTTCACTGGGATTGTGACCTGGACACCGGGGACTCTGGC
  TGCTGGCCTCACTACTCCTTCCAGCTGCAGGAGAAGAGCTACAACTTCAGGACAGCCACTCACTGGTGGG
  AGCAACCGGGTGTGGAGGCCCGCACCCTGCTCAAGCTCTATGGAATCCGCTTCGACATCCTCGTCACCGG
  GCAGGCAGGGAAGTTCGGGCTCATCCCCACGGCCGTCACACTGGGCACCGGGGCAGCTTGGCTGGGCGTG
  GTCACCTTTTTCTGTGACCTGCTACTGCTGTATGTGGATAGAGAAGCCCATTTCTACTGGAGGACAAAGT
  ATGAGGAGGCCAAGGCCCCGAAAGCAACCGCCAACTCTGTGTGGAGGGAGCTGGCCCTTGCATCCCAAGC
  CCGACTGGCCGAGTGCCTCAGACGGAGCTCAGCACCTGCACCCACGGCCACTGCTGCTGGGAGTCAGACA
  CAGACACCAGGATGGCCCTGTCCAAGTTCTGACACCCACTTGCCAACCCATTCCGGGAGCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206536 representing NM_005446
  Red=Cloning site Green=Tags(s)
  
  MCPQLAGAGSMGSPGATTGWGLLDYKTEKYVMTRNWRVGALQRLLQFGIVVYVVGWALLAKKGYQERDLE
  PQFSIITKLKGVSVTQIKELGNRLWDVADFVKPPQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWV
  DEDCPEGEGGTHSHGVKTGQCVVFNGTHRTCEIWSWCPVESGVVPSRPLLAQAQNFTLFIKNTVTFSKFN
  FSKSNALETWDPTYFKHCRYEPQFSPYCPVFRIGDLVAKAGGTFEDLALLGGSVGIRVHWDCDLDTGDSG
  CWPHYSFQLQEKSYNFRTATHWWEQPGVEARTLLKLYGIRFDILVTGQAGKFGLIPTAVTLGTGAAWLGV
  VTFFCDLLLLYVDREAHFYWRTKYEEAKAPKATANSVWRELALASQARLAECLRRSSAPAPTATAAGSQT
  QTPGWPCPSSDTHLPTHSGSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005446
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005446.3, NP_005437.2
• RefSeq Size: 2754 bp
• RefSeq ORF: 1326 bp
• Locus ID: 9127
• UniProt ID: O15547
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 22, has been identified. [provided by RefSeq, Apr 2009]