PAPOLA (NM_032632) Human Tagged ORF Clone

CAT#: RG205956

PAPOLA (tGFP-tagged) - Human poly(A) polymerase alpha (PAPOLA)

Product Images

Circular map for RG205956

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PAPOLA
• Synonyms: PAP; PAP-alpha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205956 representing NM_032632
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTTCCAGTTACAACACAGGGATCACAACAAACACAACCGCCACAGAAGCACTATGGCATTACTT
  CTCCTATCAGCTTAGCAGCCCCCAAGGAGACTGACTGCGTACTTACACAGAAACTAATTGAGACATTGAA
  ACCCTTTGGGGTTTTTGAAGAGGAAGAGGAACTGCAGCGCAGGATTTTAATTTTGGGAAAACTAAATAAC
  CTGGTAAAAGAGTGGATACGAGAAATCAGTGAAAGCAAGAATCTTCCACAATCTGTAATTGAAAATGTTG
  GAGGAAAAATTTTTACATTTGGATCTTACAGATTAGGAGTGCATACAAAAGGTGCTGATATTGATGCGTT
  GTGTGTTGCACCAAGACATGTTGATCGAAGTGACTTTTTCACCTCATTCTATGATAAGTTGAAATTACAG
  GAAGAAGTAAAAGATTTAAGAGCTGTTGAAGAGGCATTCGTACCAGTTATTAAACTCTGTTTTGATGGGA
  TAGAGATTGATATTTTGTTTGCAAGATTAGCACTGCAGACAATTCCTGAAGATTTGGATCTACGAGATGA
  CAGTCTGCTAAAAAATTTAGATATAAGATGTATAAGAAGTCTTAACGGTTGCAGGGTAACCGATGAAATT
  TTACATCTAGTACCAAACATTGACAACTTCAGGTTAACTCTGAGAGCTATCAAACTATGGGCCAAACGCC
  ACAACATCTATTCCAATATATTAGGTTTCCTCGGTGGTGTTTCCTGGGCTATGCTAGTAGCAAGAACTTG
  CCAGCTTTATCCAAATGCAATAGCATCAACTCTTGTACATAAATTTTTCTTGGTATTTTCTAAATGGGAA
  TGGCCAAATCCAGTGCTATTGAAACAGCCTGAAGAATGCAATCTTAATTTGCCTGTATGGGACCCAAGGG
  TAAACCCCAGTGATAGGTACCATCTTATGCCTATAATTACACCAGCATACCCACAACAGAACTCCACGTA
  CAATGTGTCCGTTTCAACACGGATGGTCATGGTTGAGGAGTTTAAACAAGGTCTTGCTATCACAGATGAA
  ATTTTGCTGAGTAAGGCAGAGTGGTCCAAACTTTTTGAAGCTCCAAACTTCTTTCAAAAGTACAAGCATT
  ATATTGTACTTCTAGCAAGTGCACCAACAGAAAAACAACGCCTGGAATGGGTGGGCTTGGTGGAATCAAA
  AATCCGAATCCTGGTTGGAAGCTTGGAGAAGAATGAATTTATTACACTGGCTCATGTGAATCCCCAGTCA
  TTTCCAGCACCCAAAGAAAATCCCGACAAGGAAGAATTTCGCACGATGTGGGTGATTGGGTTAGTGTTTA
  AAAAAACAGAAAACTCTGAAAACCTCAGTGTTGATCTCACCTATGATATTCAGTCTTTCACAGATACAGT
  TTATAGGCAAGCAATAAACAGCAAGATGTTTGAGGTGGATATGAAAATTGCTGCAATGCATGTAAAAAGA
  AAGCAACTCCATCAACTACTACCTAATCATGTGCTTCAGAAAAAGAAAAAGCATTCAACAGAAGGTGTCA
  AATTGACAGCTCTCAATGACAGCAGCCTCGACTTGTCTATGGACAGTGATAACAGCATGTCTGTGCCTTC
  ACCTACTAGTGCTACGAAGACCAGTCCATTGAACAGTTCTGGCAGCTCTCAGGGCAGAAACAGTCCTGCT
  CCAGCTGTAACAGCAGCATCTGTGACCAACATACAGGCTACTGAAGTTTCTGTGCCACAAGTAAATTCCA
  GTGAAAGCTCAGGGGGTACATCGAGTGAAAGCATTCCTCAAACTGCCACACAACCAGCCATTTCTCCACC
  ACCAAAGCCTACGGTCTCCAGAGTTGTTTCTTCAACACGTCTGGTAAACCCACCACCTAGATCTTCAGGA
  AATGCAGCAACTTCAGGAAATGCAGCAACAAAAATACCTACTCCTATAGTAGGAGTCAAGAGGACATCCT
  CACCTCATAAAGAAGAGAGTCCCAAGAAAACCAAAACAGAAGAGGATGAAACAAGTGAAGATGCTAACTG
  TCTTGCTTTGAGTGGACATGATAAAACAGAAGCAAAGGAACAACTTGATACAGAGACAAGTACAACTCAA
  TCAGAAACTATTCAGACAGCGGCTTCTCTGTTGGCCTCTCAGAAAACATCCAGTACAGACCTTTCTGATA
  TCCCTGCTCTCCCTGCAAATCCTATTCCTGTTATCAAGAATTCAATAAAACTGAGATTGAATCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205956 representing NM_032632
  Red=Cloning site Green=Tags(s)
  
  MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNN
  LVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQ
  EEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEI
  LHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWE
  WPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDE
  ILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQS
  FPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKR
  KQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPA
  PAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSG
  NAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQ
  SETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032632
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032632.4
• RefSeq Size: 4539 bp
• RefSeq ORF: 2238 bp
• Locus ID: 10914
• UniProt ID: P51003
• Cytogenetics: 14q32.2
• Domains: NTP_transf_2, PAP_central
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3'-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]