TLE6 (NM_024760) Human Tagged ORF Clone

CAT#: RG205815

TLE6 (tGFP-tagged) - Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 2

Product Images

Circular map for RG205815

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TLE6
• Synonyms: GRG6; PREMBL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205815 representing NM_024760
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCAGGTCCTCCGACTGGCTCCGGCGGCCTTTGGGGGAGGACAATCAGCCGGAGACCCAGCTGT
  TCTGGGACAAGGAGCCTTGGTTTTGGCACGACACTCTGACCGAGCAACTCTGGCGGATTTTTGCCGGCGT
  CCACGATGAGAAGGCAAAGCCCAGAGACAGACAGCAGGCACCAGGCCTGGGGCAGGAAAGCAAGGCACCA
  GGATCCTGTGACCCAGGAACAGACCCATGTCCTGAAGATGCCTCCACCCCCAGGCCACCTGAGGCCTCCT
  CCAGTCCCCCTGAGGGTTCCCAAGACAGGAACACAAGTTGGGGTGTGGTCCAGGAGCCTCCTGGAAGAGC
  CTCTCGGTTTCTACAGTCCATATCCTGGGACCCTGAGGACTTTGAAGATGCATGGAAGAGGCCAGATGCC
  TTGCCCGGGCAGTCAAAGAGACTCGCCGTCCCGTGCAAACTGGAAAAGATGCGGATCTTGGCACACGGGG
  AGCTCGTGCTCGCCACGGCCATCAGCAGCTTCACGCGGCACGTGTTCACCTGTGGCAGAAGAGGCATCAA
  GGTGTGGAGCCTGACTGGACAGGTGGCTGAGGACAGGTTCCCTGAGAGCCACCTGCCTATACAGACCCCT
  GGGGCCTTCCTGCGCACCTGCCTGCTGTCCTCAAACAGCAGGAGCCTGCTCACCGGTGGCTACAACCTGG
  CCAGCGTGAGCGTGTGGGACCTGGCGGCGCCCTCCCTGCATGTGAAGGAGCAGTTGCCCTGTGCAGGTCT
  CAACTGCCAGGCCCTGGATGCCAACCTGGATGCCAACCTGGCCTTCGCCAGCTTCACCAGTGGTGTGGTC
  AGGATCTGGGACCTGCGGGATCAGAGTGTGGTCAGGGACCTCAAGGGTTATCCTGATGGAGTCAAGAGTA
  TCGTGGTCAAGGGCTACAACATCTGGACTGGGGGTCCGGATGCCTGTCTGCGGTGCTGGGACCAGAGGAC
  CATCATGAAACCTCTGGAGTACCAATTCAAGTCTCAGATAATGAGCCTGTCCCACAGCCCCCAGGAGGAC
  TGGGTGCTGCTGGGCATGGCCAATGGCCAGCAGTGGCTGCAAAGCACCAGCGGGAGCCAGCGGCACATGG
  TGGGGCAAAAAGACAGCGTCATCCTGAGCGTCAAGTTCTCCCCCTTTGGCCAGTGGTGGGCAAGCGTTGG
  AATGGACGACTTCCTTGGCGTCTACAGCATGCCGGCGGGGACAAAAGTGTTCGAGGTGCCTGAGATGTCT
  CCAGTCACGTGCTGTGACGTCTCTTCCAACAACCGCCTCGTTGTCACAGGCTCCGGGGAGCACGCCTCCG
  TGTACCAGATCACCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205815 representing NM_024760
  Red=Cloning site Green=Tags(s)
  
  MATRSSDWLRRPLGEDNQPETQLFWDKEPWFWHDTLTEQLWRIFAGVHDEKAKPRDRQQAPGLGQESKAP
  GSCDPGTDPCPEDASTPRPPEASSSPPEGSQDRNTSWGVVQEPPGRASRFLQSISWDPEDFEDAWKRPDA
  LPGQSKRLAVPCKLEKMRILAHGELVLATAISSFTRHVFTCGRRGIKVWSLTGQVAEDRFPESHLPIQTP
  GAFLRTCLLSSNSRSLLTGGYNLASVSVWDLAAPSLHVKEQLPCAGLNCQALDANLDANLAFASFTSGVV
  RIWDLRDQSVVRDLKGYPDGVKSIVVKGYNIWTGGPDACLRCWDQRTIMKPLEYQFKSQIMSLSHSPQED
  WVLLGMANGQQWLQSTSGSQRHMVGQKDSVILSVKFSPFGQWWASVGMDDFLGVYSMPAGTKVFEVPEMS
  PVTCCDVSSNNRLVVTGSGEHASVYQITY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024760
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024760.3
• RefSeq Size: 1977 bp
• RefSeq ORF: 1350 bp
• Locus ID: 79816
• UniProt ID: Q9H808
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the Groucho/ transducin-like Enhancer of split family of transcriptional co-repressors. The encoded protein is a component of the mammalian subcortical maternal complex, which is required for preimplantation development. In mouse, knock out of this gene results in cleavage-stage embryonic arrest resulting from defective cytoplasmic F-actin meshwork formation and asymmetric cell division. In human, an allelic variant in this gene is associated with preimplantation embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]