KLHDC4 (NM_017566) Human Tagged ORF Clone

CAT#: RG205776

KLHDC4 (tGFP-tagged) - Human kelch domain containing 4 (KLHDC4), transcript variant 1

Product Images

Circular map for RG205776

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLHDC4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205776 representing NM_017566
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAGGGCAAGAAGGAGAAGAAGGGCCGCGGCGCGGAGAAGACGGCCGCCAAGATGGAGAAGA
  AGGTGTCTAAGCGCTCGCGGAAGGAGGAGGAAGACCTGGAAGCGCTCATAGCCCATTTCCAGACACTCGA
  TGCCAAGAGGACTCAGACTGTGGAACTTCCGTGCCCCCCACCCTCACCAAGGTTAAATGCCTCCCTCTCG
  GTTCATCCTGAGAAAGATGAGTTAATCCTTTTTGGAGGTGAATATTTCAACGGCCAAAAAACTTTTTTGT
  ATAACGAGCTCTATGTCTACAATACCAGAAAGGACACCTGGACCAAAGTTGACATCCCCAGTCCACCTCC
  GAGGCGCTGTGCTCACCAGGCGGTGGTAGTGCCTCAAGGTGGCGGACAGCTGTGGGTCTTTGGAGGGGAG
  TTTGCCTCTCCCAACGGAGAGCAGTTCTACCACTACAAGGATCTCTGGGTCCTGCATTTGGCCACCAAGA
  CCTGGGAACAAGTCAAATCAACAGGCGGTCCTTCGGGTCGGAGTGGACATCGGATGGTGGCCTGGAAGAG
  ACAATTGATCCTGTTTGGTGGCTTCCATGAAAGTACACGGGATTACATCTACTACAACGACGTGTATGCC
  TTTAATCTGGACACCTTCACATGGAGCAAGCTGTCCCCGTCAGGGACGGGGCCCACACCCAGATCAGGCT
  GCCAGATGTCCGTCACTCCCCAGGGCGGCATCGTCGTCTATGGGGGCTACTCGAAACAGAGAGTTAAGAA
  AGACGTGGACAAGGGCACACGGCACTCAGACATGTTCCTGCTGAAGCCAGAGGACGGAAGAGAAGACAAG
  TGGGTTTGGACTCGGATGAACCCTTCGGGGGTCAAGCCCACCCCACGGTCTGGCTTTTCCGTGGCCATGG
  CCCCGAATCACCAGACACTGTTCTTCGGGGGTGTCTGTGACGAGGAAGAGGAGGAGAGCCTGTCGGGCGA
  GTTCTTCAACGATCTGTACTTCTACGACGCCACCAGGAACCGTTGGTTTGAGGGACAGCTGAAGGGACCC
  AAGTCTGAAAAGAAGAAACGCAGGCGGGGCAGAAAAGAGGAGCCCGAAGGTGGTAGCAGGCCGGCGTGTG
  GGGGAGCTGGCACCCAGGGGCCTGTGCAGCTGGTCAAGGAGGTGGTGGCCGAGGATGGCACCGTGGTCAC
  CATTAAGCAGGTGCTCACCGCGCCAGGCTCGGCGGGGCAGCCCCGGTCTGAGGACGAAGACAGCCTTGAG
  GAGGCCGGCAGCCCCGCACCTGGGCCGTGTCCACGCTCCAACGCCATGCTGGCTGTGAAGCATGGGGTGC
  TCTACGTCTATGGGGGCATGTTTGAGGCCGGCGACCGCCAGGTCACCCTCAGCGACCTGCACTGCCTGGA
  CCTGCACAGGATGGAGGCGTGGAAGGCCTTGGTGGAGATGGACCCAGAAACTCAGGAGTGGCTGGAGGAG
  ACGGACTCGGAAGAGGACAGTGAGGAGGTTGAGGGCGCCGAGGGTGGGGTCGACGACGAAGACAGCGGAG
  AGGAGAGCGGTGCGGAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205776 representing NM_017566
  Red=Cloning site Green=Tags(s)
  
  MGKKGKKEKKGRGAEKTAAKMEKKVSKRSRKEEEDLEALIAHFQTLDAKRTQTVELPCPPPSPRLNASLS
  VHPEKDELILFGGEYFNGQKTFLYNELYVYNTRKDTWTKVDIPSPPPRRCAHQAVVVPQGGGQLWVFGGE
  FASPNGEQFYHYKDLWVLHLATKTWEQVKSTGGPSGRSGHRMVAWKRQLILFGGFHESTRDYIYYNDVYA
  FNLDTFTWSKLSPSGTGPTPRSGCQMSVTPQGGIVVYGGYSKQRVKKDVDKGTRHSDMFLLKPEDGREDK
  WVWTRMNPSGVKPTPRSGFSVAMAPNHQTLFFGGVCDEEEEESLSGEFFNDLYFYDATRNRWFEGQLKGP
  KSEKKKRRRGRKEEPEGGSRPACGGAGTQGPVQLVKEVVAEDGTVVTIKQVLTAPGSAGQPRSEDEDSLE
  EAGSPAPGPCPRSNAMLAVKHGVLYVYGGMFEAGDRQVTLSDLHCLDLHRMEAWKALVEMDPETQEWLEE
  TDSEEDSEEVEGAEGGVDDEDSGEESGAED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017566
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017566.4
• RefSeq Size: 1888 bp
• RefSeq ORF: 1563 bp
• Locus ID: 54758
• UniProt ID: Q8TBB5
• Cytogenetics: 16q24.2
• Domains: Kelch