SP110 (NM_004510) Human Tagged ORF Clone

CAT#: RG205648

SP110 (tGFP-tagged) - Human SP110 nuclear body protein (SP110), transcript variant b

Product Images

Circular map for RG205648

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SP110
• Synonyms: IFI41; IFI75; IPR1; VODI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205648 representing NM_004510
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACCATGACAAGAGCCATGGAAGAGGCTCTTTTTCAGCACTTCATGCACCAGAAGCTGGGGATCG
  CCTATGCCATACACAAGCCATTTCCCTTCTTTGAAGGCCTCCTAGACAACTCCATCATCACTAAGAGAAT
  GTACATGGAATCTCTGGAAGCCTGTAGAAATTTGATCCCTGTATCCAGAGTGGTGCACAACATTCTCACC
  CAACTGGAGAGGACTTTTAACCTGTCTCTTCTGGTGACATTGTTCAGTCAAATTAACCTGCGTGAATATC
  CCAATCTGGTGACGATTTACAGAAGCTTCAAACGTGTTGGTGCTTCCTATGAACGGCAGAGCAGAGACAC
  ACCAATCCTACTTGAAGCCCCAACTGGCCTAGCAGAAGGAAGCTCCCTCCATACCCCACTGGCGCTGCCC
  CCACCACAACCCCCTCAACCAAGCTGTTCACCCTGTGCGCCAAGAGTCAGTGAGCCTGGAACATCCTCCC
  AGCAAAGCGATGAGATCCTGAGTGAGTCGCCCAGCCCATCTGACCCTGTCCTGCCTCTCCCTGCACTCAT
  CCAGGAAGGAAGAAGCACTTCAGTGACCAATGACAAGTTAACATCCAAAATGAATGCGGAAGAAGACTCA
  GAAGAGATGCCCAGCCTCCTCACTAGCACTGTGCAAGTGGCCAGTGACAACCTGATCCCCCAAATAAGAG
  ATAAAGAAGACCCTCAAGAGATGCCCCACTCTCCCTTGGGCTCTATGCCAGAGATAAGAGATAATTCTCC
  AGAACCAAATGACCCAGAAGAGCCCCAGGAGGTGTCCAGCACACCTTCAGACAAGAAAGGAAAGAAAAGA
  AAAAGATGTATCTGGTCAACTCCAAAAAGGAGACATAAGAAAAAAAGCCTCCCAAGAGCCTTATCTAGAC
  ACGGAATCCAAAAGAAGCTCAAAAGGGTGGATCAGGTTCCTCAAAAGAAAGATGACTCAACTTGTAACTC
  CACGGTAGAGACAAGGGCCCAAAAGGCGAGAACTGAATGTGCCCGAAAGTCGAGATCAGAGGAGATCATT
  GATGGCACTTCAGAAATGAATGAAGGAAAGAGGTCCCAGAAGACGCCTAGTACACCACGAAGGGTCACAC
  AAGGGGCAGCCTCACCTGGGCATGGCATCCAAGAGAAGCTCCAAGTGGTGGATAAGGTGACTCAAAGGAA
  AGACGACTCAACCTGGAACTCAGAGGTCATGATGAGGGTCCAAAAGGCAAGAACTAAATGTGCCCGAAAG
  TCCAGATCGAAAGAAAAGAAAAAGGAGAAAGATATCTGTTCAAGCTCAAAAAGGAGATTTCAGAAAAATA
  TTCACCGAAGAGGAAAACCCAAAAGTGACACTGTGGATTTTCACTGTTCTAAGCTCCCCGTGACCTGTGG
  TGAGGCGAAAGGGATTTTATATAAGAAGAAAATGAAACACGGATCCTCAGTGAAGTGCATTCGGAATGAG
  GATGGAACTTGGTTAACACCAAATGAATTTGAAGTCGAAGGAAAAGGAAGGAACGCAAAGAACTGGAAAC
  GGAATATACGTTGTGAAGGAATGACCCTAGGAGAGCTGCTGAAGAGTGGACTTTTGCTCTGTCCTCCAAG
  AATAAATCTCAAGAGAGAGTTAAATAGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205648 representing NM_004510
  Red=Cloning site Green=Tags(s)
  
  MFTMTRAMEEALFQHFMHQKLGIAYAIHKPFPFFEGLLDNSIITKRMYMESLEACRNLIPVSRVVHNILT
  QLERTFNLSLLVTLFSQINLREYPNLVTIYRSFKRVGASYERQSRDTPILLEAPTGLAEGSSLHTPLALP
  PPQPPQPSCSPCAPRVSEPGTSSQQSDEILSESPSPSDPVLPLPALIQEGRSTSVTNDKLTSKMNAEEDS
  EEMPSLLTSTVQVASDNLIPQIRDKEDPQEMPHSPLGSMPEIRDNSPEPNDPEEPQEVSSTPSDKKGKKR
  KRCIWSTPKRRHKKKSLPRALSRHGIQKKLKRVDQVPQKKDDSTCNSTVETRAQKARTECARKSRSEEII
  DGTSEMNEGKRSQKTPSTPRRVTQGAASPGHGIQEKLQVVDKVTQRKDDSTWNSEVMMRVQKARTKCARK
  SRSKEKKKEKDICSSSKRRFQKNIHRRGKPKSDTVDFHCSKLPVTCGEAKGILYKKKMKHGSSVKCIRNE
  DGTWLTPNEFEVEGKGRNAKNWKRNIRCEGMTLGELLKSGLLLCPPRINLKRELNSK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004510
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004510.2, NP_004501.2
• RefSeq Size: 1962 bp
• RefSeq ORF: 1650 bp
• Locus ID: 3431
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Jul 2008]